The following chemicals were used for the analysis of DON and its metabolites: 13C labelled DON standard (Sigma, Saint Louis, MO, USA; product number: 34128, 1.2 mL); DON (Sigma, Saint Louis, MO, USA; product number: D0156, 1 mg); β-glucuronidase (Type IX-A from E. coli; Sigma, Saint Louis, MO, USA; product number: G7396-2MU); DOM-1 (Sigma, Saint Louis, MO, USA; product number: 34135, 2 mL); and DONtest WBTM immunoaffinity columns (Vicam, Milford, MA, USA; product number: G1066). The equipment used was a Waters 2795 HPC Separation Module (Waters Corp., Milford, MA, USA) with a Quattro Micro Triple Quadrupole Mass Spectrometer (Micromass UK Ltd., Manchester, UK).
Dontest wbtm immunoaffinity columns
Manufactured by VICAM
Sourced in United States
The DONtest WBTM immunoaffinity columns are designed for the detection and quantification of deoxynivalenol (DON), a mycotoxin produced by certain Fusarium fungi. The columns utilize a specific antibody that binds to DON, allowing for its isolation and concentration from sample extracts. This product provides a reliable tool for analyzing the presence of DON in various matrices.
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2 protocols using dontest wbtm immunoaffinity columns
1
Analysis of DON and Metabolites
2
Quantitative Analysis of DON and Metabolites
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DON and its metabolites were analysed using 13 C labelled DON standard (Sigma, Saint Louis, MI, USA; product number: 34128, 1.2 mL), DON (Sigma, Saint Louis, MI, USA; product number: D0156, 1 mg), β-glucuronidase (Type IX-A from E. coli; Sigma, Saint Louis, MI, USA; product number: G7396 -2MU), DOM-1 (Sigma, Saint Louis, MI, USA; product number: 34135, 2 mL), and DON test WB TM immunoaffinity columns (Vicam, Milford, MA, USA; product number: G1066). All analyses were conducted on a Waters 2795 HPC Separation Module (Waters Corp., Milford, MA, USA) with a Quattro Micro Triple Quadrupole Mass Spectrometer (Micromass UK Ltd., Manchester, UK).
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