Anti akt1 phospho s473 antibody

L-Nitro-arginine-methyl-ester (L-NAME) was purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany; batch no. N5751-10), and sodium pentobarbital was purchased from Shanghai Chemical Reagent Co., Ltd. (Shanghai, China). Anti-HIF-1 alpha antibody, anti-PTEN antibody, anti-VEGFA antibody, anti-PI3K p85 antibody, anti-PI3K p85 (phospho Y607) antibody, and anti-AKT1 (phospho S473) antibody were purchased from Abcam (batch no. Ab1; ab32199; ab1316; ab86714; ab182651; ab81283; dilution 1 : 200; 1 : 100; 1 : 100; 1 : 100; 1 : 200; 1 : 200); TRIB3 polyclonal antibody, AKT (L321) polyclonal antibody, and AKT (phospho-T308) polyclonal antibody were purchased from Bioworld (batch no. BS60451; BS1502; BS4647; dilution 1 : 100; 1 : 100; 1 : 100).

Small molecule compound 7460-0250 and MK-2206 were bought from Taosu Bio LTD. (China). Anti-AKT1 (phospho S473) antibody and anti-AKT2 (phospho S474) antibody were bought from Abcam (USA). Anti-Bax antibody and anti-mTOR antibody were from CST (USA). Anti-AKT3 (phospho S472) antibody was from Abnova (China). Annexin V -FITC Apoptosis Kit was purchased from solarbio (China). 10% fetalbovine serum and crystal violet were from Beyotime (China). Cell counting kit-8 was from Beyotime (China).