Change from baseline over time in the following type 2 biomarkers was assessed and reported in the QUEST and SINUS‐52 studies as pre‐specified end‐points:22 (link), 23 (link) serum TARC (human TARC Quantikine ELISA kit; R&D Systems); plasma eotaxin‐3 (human eotaxin‐3 Quantikine ELISA kit; R&D Systems); serum total IgE (ImmunoCAP® fluorescence enzyme immunoassay method; Phadia AB); serum periostin (ELISA kit; Shino‐Test); and blood eosinophil count (measured as part of the standard 5‐part white blood count differential cell count on a haematology autoanalyzer). QUEST also assessed FeNO (NIOX instrument; Aerocrine AB) and eosinophil cationic protein (ECP); (ImmunoCAP® fluorescence enzyme immunoassay method; Phadia AB). CHRONOS, SOLO‐1 and SOLO‐2 also assessed the effect of treatment on serum TARC, serum total IgE as exploratory end‐points and blood eosinophils as pre‐specified laboratory testing.19 (link), 20 (link), 21 (link) The EoE PoC study assessed the effect of treatment on serum TARC, serum total IgE, serum periostin and blood eosinophils from samples collected for exploratory research.24 (link) Allergen‐specific IgE levels were also measured in the assessed studies. However, these data have not been included in the current analysis, as kinetics are dependent on allergen exposure (eg season, region).20 (link)
Human tarc quantikine elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human TARC Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed to measure human TARC (Thymus and Activation-Regulated Chemokine) levels in cell culture supernates, serum, and plasma.
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2 protocols using human tarc quantikine elisa kit
1
Biomarker Changes in Type 2 Asthma Studies
2
Analyzing EGF and TARC in hUCB-MSCs
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hUCB-MSCs (2×106) were suspended in 0.6 ml DMEM containing 10% FBS and 100 μg/ml primocin, transferred to 24-well plate in duplicates, and cultured for 24 h. Thereafter, the culture medium was collected and centrifuged at 500 g for 5 min. The concentration of EGF was measured using a Human EGF Quantikine ELISA Kit (R&D Systems) according to the manufacturer’s instructions. In addition, 1×105 HaCaT cells/well were co-cultured with 5×105 hUCB-MSCs/transwell (0.4 μm polyethylene terephthalate membrane; Falcon, USA) in 24-well plates and stimulated with TNF-α/IFN-γ (each at 10 ng/ml) for 24 h. The concentration of TARC was measured using a Human TARC Quantikine ELISA Kit (R&D Systems) according to the manufacturer’s instructions.
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