The Oroboros O2K Oxygraph High-Resolution Respirometer was used to determine the consumption of O 2 in the cell suspensions. Datlab 4 software was used for the analysis and acquisition of data generated by the respirometer. Cell suspensions were diluted to a cell density of 10 6 cells/mL and a routine respiration value (routine) was obtained, followed by the administration of the following respiratory chain inhibitors, in order: 0.2 mM triethiltyn bromide (TET, Sigma-Aldrich, UK), a complex V inhibitor used to assess respiration levels not linked to proton movement across the ATP synthase, referred to as LEAK respiration; 12 µM carbonylcyanidep-trifluoromethoxyphenylhydrazone (FCCP, Fluka, UK), a proton ionophore that reveals maximum (or uncoupled) respiration, referred to as ETS respiration; 2 µM antimycin A (AntA, Sigma-Aldrich), a complex III inhibitor that shows NMT or non-mitochondrial respiration. All experiments were performed in biological triplicate, as indicated.
Antimycin a anta
Manufactured by Merck Group
Sourced in United Kingdom, United States
Antimycin A (AntA) is a chemical compound produced by certain bacteria. It functions as an inhibitor of the mitochondrial electron transport chain, specifically targeting the cytochrome bc1 complex. This disrupts cellular respiration and ATP production in various organisms.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using antimycin a anta
1
High-Resolution Respirometry of Cellular O2 Consumption
2
Preparation and Storage of Bioactive Compounds
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Simvastatin lactone (Sigma-Aldrich, St-Louis, USA) was converted to the active acid form by hydrolysis [37] (link) for cell experiments. Stock solutions were obtained after dilution in DMSO (Sigma-Aldrich, USA) and stored at -20°C. Human insulin was purchased from Sigma-Aldrich, stored at 4°C and stock solutions were prepared in medium. Rapamycin was purchased from Sigma-Aldrich and diluted in DMSO. Mevalonolactone (MEVA) (Sigma-Aldrich, USA) was diluted in water and stocks were stored at -20°C. Farnesol (FOH) was purchased from Sigma-Aldrich (Sigma-Aldrich, USA) and stock solutions were prepared in DMSO. Geranylgeraniol (GGOH) was purchased from Sigma-Aldrich and stock solutions were prepared in ethanol. MitoTEMPO (MITOT.) was obtained from Sigma-Aldrich and stocks were obtained after dilution in water. Antimycin A (Ant.A) (Sigma-Aldrich, St-Louis, USA) stock solutions were obtained after preparation in ethanol.
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