Gastric normal fibroblasts (GNFs) and GCAFs grown on glass coverslips were washed with cold phosphate-buffered saline (PBS), fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and then incubated with primary antibodies to pan-CK (1:100), E-cadherin (1:100), vimentin (1:150), PDGFR-β (1:100), and α-SMA (1:100) overnight at 4 °C. The cells were subsequently incubated with fluorescence-conjugated secondary antibodies for 1 h at 37 °C. After three PBS washes, cell nuclei were counterstained with DAPI for 5 min; the cells were examined with a Nikon Eclipse Ti-S Epifluorescence microscope equipped with a DS-L2 digital camera (Nikon).
Ds l2 digital camera
Manufactured by Nikon
The Nikon DS-L2 digital camera is a versatile laboratory equipment designed for scientific imaging applications. It features a high-resolution sensor, adjustable magnification, and advanced optics to capture detailed images with precision. The DS-L2 is suitable for a range of laboratory tasks, including microscopy, material analysis, and quality control.
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Lab products found in correlation
2 protocols using ds l2 digital camera
1
Immunofluorescence Analysis of Gastric Fibroblasts
2
Fungal Isolation and Morphological Characterization
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Eight isolates obtained from fungal substrates were prepared for microscopic morphological identification using the methods described by Zare et al. [14] (link) with reference to Gams et al. [40] . Fungal structures were observed in preparations mounted using MilliQ. Field and macroscopic images were obtained using a X-M1 mirrorless digital camera (Fujifilm, Tokyo, Japan) with a QZ-35M lens (TAMRON, Saitama, Japan), a COOLPIX 4500 compact digital camera (Nikon, Tokyo, Japan), and a SMZ-10A stereomicroscope (Nikon) with a DP12 digital camera (Olympus, Tokyo, Japan). The morphological characteristics of the isolates were observed and recorded using an OPTIPHOT2 microscope with a differential interference contrast device and a DS-L2 digital camera (Nikon). To characterize colony morphology, PDA and 2% malt extract agar (MEA; Becton, Dickinson, and Company) were used.
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