To determine the fatty acid composition, fatty acid methyl esters (FAMEs) were produced by esterification of extracted fats with methanolic KOH solution by adding 20 % of BF3-methanol solution (Sigma-Aldrich) as described by Ssepuuya et al. [14] (link). Fatty acid composition was measured by a GC-MS (GC 7820A/5977E MSD, Agilent Technologies, USA) fitted with a SLB-IL60 column (30 m x 0.25 mm x 0.2 µm, Sigma-Aldrich, St. Louis, USA) with helium as carrier gas. The initial oven temperature was 40 °C for 4 minutes, which subsequently increased at 5 °C/min up to 280 °C and the run time was 55 minutes. Analytical standards (Sigma Aldrich) for each fatty acid were used for calibration. The MS setting was in SIM mode with electron impact ionization of 70 eV. Fatty acids were quantified by MassHunter software (Agilent Technologies, Santa Clara, USA) while methyl tricosanoate (Sigma Aldrich) was used as the internal standard.
Slb il60 column
Manufactured by Merck Group
Sourced in United States
The SLB-IL60 column is a liquid chromatography column designed for efficient separation and purification of various chemical compounds. It features a 60 mm internal diameter and is constructed with a stationary phase suitable for a range of applications. The column's core function is to facilitate the separation and isolation of target analytes from complex mixtures through liquid-based chromatographic techniques.
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2 protocols using slb il60 column
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Fatty Acid Composition Analysis by GC-MS
2
Fatty Acid Composition Analysis by GC-MS
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To determine the fatty acid composition, fatty acid methyl esters (FAMEs) were produced by esterification of extracted fats with methanolic KOH solution by adding 20 % of BF3-methanol solution (Sigma-Aldrich) as described by Ssepuuya et al. [14] (link). Fatty acid composition was measured by a GC-MS (GC 7820A/5977E MSD, Agilent Technologies, USA) fitted with a SLB-IL60 column (30 m x 0.25 mm x 0.2 µm, Sigma-Aldrich, St. Louis, USA) with helium as carrier gas. The initial oven temperature was 40 °C for 4 minutes, which subsequently increased at 5 °C/min up to 280 °C and the run time was 55 minutes. Analytical standards (Sigma Aldrich) for each fatty acid were used for calibration. The MS setting was in SIM mode with electron impact ionization of 70 eV. Fatty acids were quantified by MassHunter software (Agilent Technologies, Santa Clara, USA) while methyl tricosanoate (Sigma Aldrich) was used as the internal standard.
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