Antihuman pd l1 neutralizing antibody

Manufactured by BioLegend

The Antihuman PD-L1 neutralizing antibody is a monoclonal antibody that specifically binds to and neutralizes the programmed death-ligand 1 (PD-L1) protein. PD-L1 is a key regulator of the immune response and its inhibition can enhance T-cell activation and anti-tumor immunity.

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Lab products found in correlation

Isotype control antibody, by BioLegend (1 mentions) Anti cd3, by Thermo Fisher Scientific (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Selleck Chemicals (1 mentions) 1 methyl d tryptophan 1 mt, by Merck Group (1 mentions) Mouse igg2b control, by BioLegend (1 mentions) Ki 67 staining, by BioLegend (1 mentions)

Close spelling variants of this product

PD-L1 neutralizing antibody PD-L1 antibodies PD-L1

2 protocols using antihuman pd l1 neutralizing antibody

Autologous T Cell Activation and Proliferation

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Autologous T cells were labeled with 5 µM carboxyfluorescein succinimidyl ester (CFSE, Selleck Chemicals) for 25 min at 4°C and preactivated with plate-bound 2 µg/mL anti-CD3 (Thermo Fisher) at 37°C, 5% CO₂ for 16–18 hours. Three days after activation, the T cells were incubated with the macrophages for 2 days at a T cell:macrophage ratio of 5:1. The proliferation of T cells was measured by CFSE staining and flow cytometry. In some experiments, 5 mg/mL antihuman PD-L1 neutralizing antibody (Biolegend), 5 mg/mL isotype control antibody (Biolegend) or 4 mg/mL 1-MT (Selleck Chemicals) was added in the coculture.

Liu D., Xu X., Dai Y., Zhao X., Bao S., Ma W., Zha L., Liu S., Liu Y., Zheng J, & Shi M. (2021). Blockade of AIM2 inflammasome or α1-AR ameliorates IL-1β release and macrophage-mediated immunosuppression induced by CAR-T treatment. Journal for Immunotherapy of Cancer, 9(1), e001466.

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Modulating NK Cell Proliferation

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Autologous NK cells labeled with CTDR were cultured alone or co-cultured with macrophages with indicated treatments (2:1) in complete media (RPMI 1640 medium supplemented with 10% FBS) and stimulated with 100U/ml IL-2 and 50U/mL PeproTech) for 4 days. Afterward, the proliferation rate was evaluated by flow cytometry for Ki-67 staining (Cat# 350503, Biolegend) . In some experiments, 5 mg/ml anti-human PD-L1 neutralizing antibody (Cat# 329709, Biolegend), 5 mg/ml mouse IgG2b control (Cat# 400301, Biolegend) or 4 mg/ml 1-Methyl-D-tryptophan (1-MT, Cat# 452483, Sigma) was added in the co-culture.

Su S., Zhao J., Xing Y., Zhang X., Liu J., Ouyang Q., Chen J., Su F., Liu Q, & Song E. (2018). Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages. Cell, 175(2).

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