Male Sprague-Dawley rats (7-, 18-, 28-and 60-days of age) were purchased from Charles River Laboratories, Inc. (Fair eld, New Jersey). Rats were acclimated for one week under constant photoperiod (12h light: 12h dark), and received food and water ad libitum. At the time of sampling, rats were anesthetized with CO 2 and killed by cervical dislocation or decapitation. Epididymides were dissected from rats under aseptic conditions and either frozen in liquid nitrogen and stored at -86ºC or placed in Dulbecco Modi ed Eagles Medium (DMEM)/HAM F12 culture media containing penicillin (50U/mL) and streptomycin (50µg/mL) (Sigma-Aldrich, Oakville, ON). All the animal protocols used in this study were approved by the university animal care committee.
Dmem ham f12 culture media
Manufactured by Merck Group
DMEM/HAM F12 culture media is a cell culture medium designed to support the growth and maintenance of a variety of cell types. It is a combination of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F12 nutrient mixture, which provides a balanced formulation of amino acids, vitamins, salts, and other essential components required for cell growth and survival.
Automatically generated - may contain errors
2 protocols using dmem ham f12 culture media
1
Rat Epididymis Dissection and Preservation
2
Rat Epididymis Dissection and Preservation
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Male Sprague-Dawley rats (7-, 18-, 28-and 60-days of age) were purchased from Charles River Laboratories, Inc. (Fair eld, New Jersey). Rats were acclimated for one week under constant photoperiod (12h light: 12h dark), and received food and water ad libitum. At the time of sampling, rats were anesthetized with CO 2 and killed by cervical dislocation or decapitation. Epididymides were dissected from rats under aseptic conditions and either frozen in liquid nitrogen and stored at -86ºC or placed in Dulbecco Modi ed Eagles Medium (DMEM)/HAM F12 culture media containing penicillin (50U/mL) and streptomycin (50µg/mL) (Sigma-Aldrich, Oakville, ON). All the animal protocols used in this study were approved by the university animal care committee.
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