Polyclonal rabbit anti lox ab31238 antibody

Recombinant human activin A was obtained from R&D Systems and was composed of a Chinese hamster ovary cellderived recombinant human protein that was >95% pure based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with silver staining. It was supplied lyophilized from a 0.2 μM filtered solution of 4 mM HCL with 0.1% BSA as a carrier protein. The activin/TGF-β type I receptor inhibitor SB431542 was purchased from Sigma-Aldrich. Polyclonal goat anti-CTGF antibody (sc-34772) (diluted at 1:1000), and monoclonal mouse anti-α tubulin antibody (sc-23948) (diluted at 1:2000) were obtained from Santa Cruz Biotechnology. Polyclonal rabbit anti-LOX (ab31238) antibody (diluted at 1:1000) was obtained from Abcam. A horseradish peroxidaseconjugated goat anti-rabbit and goat anti-mouse IgGs were obtained from Bio-Rad Laboratories. Horseradish peroxidaseconjugated donkey anti-goat IgG was obtained from Santa Cruz Biotechnology.

Recombinant human TGFB1 was obtained from R&D Systems as a Chinese hamster ovary cell-derived protein of more than 97% purity based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Lyophilized TGFB1 was reconstituted in a solution of 4 mM HCl with 0.1% BSA as a carrier protein. The TGF-β type I receptor inhibitor SB431542 (S4317) was purchased from Sigma-Aldrich. Monoclonal mouse anti-α-tubulin antibody (sc-23948; diluted 1:2000) was obtained from Santa Cruz Biotechnology. The polyclonal rabbit anti-LOX (ab31238) antibody (diluted 1:1000) was obtained from Abcam. The horseradish peroxidase-conjugated goat anti-rabbit and goat anti-mouse immunoglobulin G antibodies were obtained from Bio-Rad Laboratories.