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200 nm filter

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The 200-nm filter is a laboratory filter designed to remove particles and contaminants from liquids. It has a nominal pore size of 200 nanometers, which allows the passage of dissolved substances while effectively trapping smaller suspended particles. This filter can be used for a variety of filtration applications in research and industrial settings.

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200 nm polycarbonate filters (3 citations) 200 nm polycarbonate membrane filter (3 citations)

2 protocols using 200 nm filter

1

Liposome-Protein Interaction Visualization

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Liposomes for negative staining were generated at 1.5 mM as described in liposome cosedimentation assays with 10% PI(3)P and extruded through a 200-nm filter (Avanti Polar Lipids). Liposomes (0.5 mM) were incubated with protein for 30 min. Samples were applied to copper grids coated with continuous carbon, negatively stained with 2% uranyl acetate (JT Baker Chemical), and imaged using a Morgagni 268 transmission electron microscope (FEI) operating at 80 kV and equipped with a 1,000 × 1,000 CCD camera (Gatan).
Wang S., Zhao Z, & Rodal A.A. (2019). Higher-order assembly of Sorting Nexin 16 controls tubulation and distribution of neuronal endosomes. The Journal of Cell Biology, 218(8), 2600-2618.
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2

Exosome-Platelet Membrane Hybrid Formation

Cited 1 time
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The protein content of exosomes was determined with a BCA protein assay. Exosomes (XOs, 0.5 mL, 1010/mL) and platelet membrane fractions (PM, 0.5 mL) were mixed at different protein content ratios (PM:XOs=1:2, 3:4 and 1:1) in the presence of polyethylene glycol (5%).[52 (link)] To modify the platelet membranes onto the surface of exosomes, the mixture was extruded 12 times through a 200 nm filter (Avanti Polar Lipids, Inc). The number and size of the XOs and the P-XOs were analyzed using a NanoSight LM10 nanoparticle analyzer. The hybrid efficiency was determined with flow cytometry. In brief, P-M were labeled with DiO (1 μL, 100 ug/mL) and XOs were labeled with Dil (1 μL, 1010/mL) before the modification. The composition of the resulting P-XOs was analyzed with flow cytometry (vSSC mode, CytoFlex). The purity is calculated as P-XOs/(XOs+P-XOs) ×100%. The efficiency is as particle numbers of final solution/numbers of input XOs ×100%.
Hu S., Wang X., Li Z., Zhu D., Cores J., Wang Z., Li J., Mei X., Cheng X., Su T, & Cheng K. (2021). Platelet membrane and stem cell exosome hybrid enhances cellular uptake and targeting to heart injury. Nano today, 39, 101210.
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