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2 protocols using 1 2 distearoyl sn glycero 3 phosphate

1

Preparation and Characterization of RN18 Microbubbles

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RN18 microbubbles were prepared according to the previously described protocol [18 (link)]. The MB shells were comprised of lipids at a 82:10:8 M ratio of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-distearoyl-sn-glycero-3-phosphate (DSPA), and N-(Carbonylmethoxypolyethyleneglycol 5000)-1,2-distearoyl-sn-glycero-e-phosphoethanolamine (MPEG-5000-DSPE) purchased from Avanti® Polar Lipids, Inc. (Alabaster, AL). Before use, MBs were produced by vigorous agitation of the lipid emulsion for 45 s using a Vialmix™ (Lantheus Medical Imaging, N. Billerica, MA), yielding an average concentration of 2–5 × 109 MBs/mL.
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2

Phospholipid Library Characterization

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L-α-PC from egg yolk, 1,2-dipalmitoyl-sn-glycero-3-phosphoserine (16:0/16:0-PS), 1,2-dipalmitoyl-sn-glycero-3-phosphate (16:0/16:0-PA), 1,2-distearoyl-sn-glycero-3-phosphate (18:0/18:0-PA), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphate (16:0/18:1-PA), 1-stealoyl-2-oleoyl-sn-glycero-3-phosphate (18:0/18:1-PA), 1,2-dioleoyl-sn-glycero-3-phosphate (18:1/18:1-PA), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphate (18:0/20:4-PA), and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphate (18:0/22:6-PA) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Cholesterol (Chol) was purchased from Wako Pure Chemical Industries (Tokyo, Japan). Membrane Lipid Strips were purchased from Echelon Biosciences (Salt Lake City, UT, USA).
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