Ab match assembly human pap1 kit

Manufactured by MBL Life Science

Sourced in United States

The Ab-Match Assembly Human PAP1 kit is a laboratory product designed for the assembly of antibodies. It provides the necessary components and instructions to facilitate the assembly process. The core function of this kit is to enable the assembly of antibodies, without further interpretation or extrapolation on its intended use.

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Lab products found in correlation

Ab match universal kit, by MBL Life Science (3 mentions) Synergy 2, by Agilent Technologies (1 mentions) Clariostar reader, by BMG Labtech (1 mentions) Mars data analysis software, by BMG Labtech (1 mentions)

3 protocols using ab match assembly human pap1 kit

REG3α ELISA Quantification Protocol

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REG3α ELISA kits from MBL International Corporation (Ab-Match Assembly Human PAP1 kit, catatolog number 5323, and Ab-Match Universal kit, catalog number 5310, Woburn, Mass., USA) were used. All measurements were performed according to the manufacturer's protocol. All samples were diluted 1: 10 according to the protocol and as recommended, all calibrators, controls, and patient samples were analyzed in duplicate. One standard curve per assay was made. Absorbance of each well was read at test wavelength of 450 and 620 nm with the multi detection microplate reader Synergy 2 (Biotek Instruments, Winooski, Vt., USA). Results were calculated with Gen 5 version 1.11 (Biotek Instruments). Only values within the range of the standard curve were used for further analysis. If duplicates varied more than 16%, ELISA was repeated for this sample.

Greuter T., Lang S., Holler E., Hawkey C.J., Rogler G, & Biedermann L. (2015). Serum REG3alpha and C-Reactive Protein Levels in Crohn's Disease Patients Undergoing Immunoablation and Autologous Hemopoetic Stem Cell Transplantation in the ASTIC Trial. Digestion, 92(2).

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Serum Biomarkers for Disease Monitoring

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Concentrations of Reg3α (PAP1; Ab-Match Assembly Human PAP1 kit and Ab-Match Universal kit; MBL International, Des Plaines, IL, USA), CK18F (M30 Apoptosense ELISA; Peviva AB, Sundbyberg, Sweden), and elafin (R&D Systems, Minneapolis, MN, USA) were measured from patient and healthy control serum samples by sandwich ELISA according to the manufacturers’ protocols. Serum samples with very high protein concentrations were diluted to match the protocols’ ranges of detection. Absorbance at 450 nm was measured by using the ClarioStar reader (BMG Labtech, Ortenberg, Germany). Samples were run in triplicate. Analyses were carried out with the Mars Data Analysis software (BMG Labtech).

Keto J., Kaartinen T., Salmenniemi U., Castrén J., Partanen J., Hänninen A., Korhonen M., Lähteenmäki K., Itälä-Remes M, & Nystedt J. (2018). Immunomonitoring of MSC-Treated GvHD Patients Reveals Only Moderate Potential for Response Prediction but Indicates Treatment Safety. Molecular Therapy. Methods & Clinical Development, 9, 109-118.

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Biomarkers for GVHD Prediction and Monitoring

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Elafin and tumor necrosis factor receptor 1 (TNFR1) antibody pairs were purchased from R&D Systems (DuoSet) and measurements were performed according to the manufacturer’s protocol with a plasma dilution of 1:25 and 1:20. Regenerating islet-derived 3-α (REG 3α) ELISA kit was purchased from MBL International (Ab-Match Assembly Human PAP1 kit and Ab-Match Universal kit), and measurements were according to the manufacturer’s protocol with a plasma dilution of 1:10 to 20. Cytokeratin fragment 18 ELISA kit was purchased from Peviva AB (M30 Apoptosense ELISA) and measurements were performed according to the manufacturer’s protocol with a plasma dilution of 1:25. Samples and standards were run in duplicate, absorbance was measured at 450 nm determined using a reader of Perkin Elmer Co. The absorbance data were analyzed using Excel. REG 3α and TNFR1 were significantly higher in patients 2, 3, 4, 6, 10 who mainly manifested gastrointestinal GVHD than the 6 controls (p=0.027 and p=0.008). CK18 was significantly higher in patients 1, 2, 4, 5, 6, 7, and 8 who developed liver GVHD or tissue injury (p=0.044) and Elafin was higher in patients 1, 3, 6, 8 who developed acute or chronic skin GVHD than the 6 controls (p=0.027, Figure S1).

Yin F., Battiwalla M., Ito S., Feng X., Chinian F., Melenhorst J.J., Koklanaris E., Sabatino M., Stroncek D., Samsel L., Klotz J., Hensel N.F., Robey P.G, & Barrett A.J. (2014). Bone marrow mesenchymal stromal cells to treat tissue damage in allogeneic stem cell transplant recipients: Correlation of biological markers with clinical responses. Stem cells (Dayton, Ohio), 32(5), 1278-1288.

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