DMPC and NBD-DMPE were purchased from Avanti Polar Lipids (Alabaster, AL). DPH was purchased from Sigma-Aldrich (St. Louis, MO). D2O was purchased from Cambridge Isotope Laboratories (Tewksbury, MA). All other reagents were of standard laboratory grade.
Nbd dmpe
Manufactured by Avanti Polar Lipids
Sourced in United States
NBD-DMPE is a fluorescently-labeled phospholipid used in liposome and membrane studies. It consists of a phosphatidylethanolamine (PE) headgroup with a nitrobenzoxadiazole (NBD) fluorescent label attached to the acyl chain. NBD-DMPE can be used to monitor membrane properties and dynamics in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Poly l lysine hbr pll, by Merck Group (1 mentions)
Pd spintrap g 25, by GE Healthcare (1 mentions)
Anti hsc70, by Enzo Life Sciences (1 mentions)
Aldehyde sulfate latex beads, by Thermo Fisher Scientific (1 mentions)
Ecl western blotting detection reagent, by GE Healthcare (1 mentions)
Dotap, by Avanti Polar Lipids (1 mentions)
3 protocols using nbd dmpe
1
Lipid Membrane Preparation and Analysis
2
Polycation-Lipid Nanomaterial Synthesis
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Sodium hyaluronate (HA, Lifecore Biomedical, 360 kDa), poly-L-lysine HBr (PLL, Sigma), PLL of 15-30 kDa (PLL28, where the average M w is 28 kDa) and PLL of 150-300 kDa (PLL280) were used. Poly(ethylenimine) (PEI, Sigma), 2-oleoyl-1-palmitoylsn-glycero-3-phosphocholine (POPC, Avanti Polar Lipids, M w : 785.6), 2-oleoyl-1-palmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (POPG, Avanti Polar Lipids, M w : 771.0), N-(NBD-aminododecanoyl)-1,2-ditetradecanoyl-sn-glycero-3-phosphoethanolamine sodium salt (NBD-DMPE, Avanti Polar Lipids, M w: 816.0), cholesterol (Chol, Sigma, M w : 386.7), lysozyme (chicken egg white, CalBioChem, M w : 14.3 kDa), and α-lactalbumin (bovine milk, type II, calcium depleted, Sigma, M w : 14.2 kDa) were also used. 10 mM Tris buffer solution containing 15 mM NaCl at pH 7.4 and 0.1 M carbonate buffer solution at pH 9.0 were prepared using distilled water and chemicals purchased from Sigma. Other chemicals and reagents were of analytical grade.
Chemical structures of the lipids and polymers used in this study are provided in Fig. S1. †
Chemical structures of the lipids and polymers used in this study are provided in Fig. S1. †
3
Exosome Characterization Protocols
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DOPC, DOPS, and PEG-DSPE, were purchased from NOF Corp. Ltd. (Tokyo, Japan). DOTAP (chloride salt), NBD-DMPE, and rho-DMPE were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Anti-Hsc70 (Anti-Hsc70 rat monoclonal antibody [1B5]), anti-HER2 (anti-ErB2), anti-CD9, anti-phosphorylated HER2 (p-Neu[Tyr1248]), phycoerythrin (PE)-conjugated mouse anti-human CD63, horseradish peroxidase (HRP)-conjugated goat-anti rabbit-IgG, and PE-conjugated mouse IgG1 were purchased from Enzo Life Sciences (Farmingdale, NY, USA), abcam (Cambridge, UK), Santa Cruz Biotechnology (Santa Cruz, CA, USA), Biolegend (San Diego, CA, USA), and BD Bioscience (San Jose, CA, USA), respectively. ECL Western Blotting Detection Reagent and PD SpinTrap G-25 were purchased from GE Healthcare Japan (Tokyo, Japan). Aldehyde/sulfate latex beads (4% w/v, 4 μm) and stain buffer (FBS) were purchased from Life Technologies (Carlsbad, CA, USA) and BD Bioscience (CA, USA), respectively. CFSE was obtained from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan).
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