Pd98059

Nocodazole and peroxovanadate (VOOH) were purchased from Sigma. EGF was purchased from R&D systems. Y27632 was purchased from Tocris Bioscience and the MEK inhibitor, PD98059 was purchased from A.G. Scientific. The reporter constructs 5XcollTRETATA-Luc (TRE-TATA), 5Xjun2TRE-TATA-Luc (Jun2-TATA) and TATA-Luc (TATA) [32 (link)] were a gift from P. Angel (Heidelberg, Germany). The expression vector for dominant-negative c-Jun, pEGP-TAM67 [4 (link)], was kindly provided by R.F Hennigan, (New York, USA). Expression vector for miR-10b [40 (link)] was provided by Agami R. (The Netherlands Cancer Institute, Amsterdam). TK-Rnl (Promega), pCDNA3 (Invitrogen) and pEGFP-C3 (Clontech) are commercial vectors. The expression vector for NF1-GRD-GFP was provided by Kloog Y. (Tel Aviv University, Tel Aviv) and the full length NF1 (NF1-GFP) was a gift from McCormick F. (University of California, San Francisco). Constitutively active RhoC construct, RhoC G14V 3XHA-tagged, was purchased from Missouri S&T cDNA Resource Center. Anti-hsa-miR-10b (miScript miRNA Inhibitor) and control oligonucleotide were purchased from Qiagen. A panel of shRNA constructs for c-Jun and a control vector encoding non-effective 29-mer cassette, were purchased from OriGene Technologies, Inc. shRNA constructs with the strongest effect on c-Jun (c-Jun 5 and c-Jun 7) were used for further experiments.

Crocin (Figure 1(a)), LPS (phenol extracted from Salmonella enteritidis), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), actinomycin D (Act. D), cycloheximide (CHX), 2-(3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl)benzoic acid acetoxymethyl ester (Fluo-3/AM), and other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cobalt protoporphyrin (CoPP) and zinc protoporphyrin IX (ZnPP) were bought from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). LY294002, PD98059, SP600125, and SB203580 were obtained from A.G. Scientific (San Diego, CA, USA). Nifedipine, 1,2-bis (o-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA/AM), and calmidazolium chloride were acquired from Calbiochem (EMD Millipore, Billerica, MA, USA). KN92, KN93, and KN62 were purchased from Cayman Chemical (Ann Arbor, MI, USA) and Calbiochem.