Caspase 3 activity detection kit

Antibodies specific for Bcl-2, Bak-1 and β-actin were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Secondary antibodies conjugated to horseradish peroxidase were purchased from Thermo Fisher Scientific, Inc. (New York, NY). The Annexin V-conjugated FITC apoptosis detection kit was obtained from BD Pharmaceuticals (Franklin Lakes, NJ). The JC-1 mitochondrial membrane potential detection kit and Caspase-3 activity detection kit were purchased from NanJing KeyGen Biotech Co., Ltd. (Nanjing, China), and the in situ cell death detection kit was purchased from Roche Diagnostic Corporation (Indianapolis, IN). MTT (3-(4,5-dimethyl-2-yl)-2,5-diphenyl tetrazolium bromide), PI (Propidium iodide) and DAPI (2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride) were obtained from Sigma Chemical Co. (St. Louis, MO). The GSPs were purchased from Jianfeng Natural Product R&D Co., Ltd. (Tianjin, China).

IMDM was from Invitrogen (Carlsbad, CA). siRNA of NR4A2 and negative control were from GenePharma (Shanghai, China). Lipofectamine 2000 was from Invitrogen (Carlsbad, CA). NR4A2 and β-actin antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for caspase3, PARP1, p-P65, P65, p-IκBα, IκBα, LC3 were from Cell Signaling Technology (Beverly, MA, USA). 3-methyladenine (3MA) and N-acetyl cysteine (NAC) were from Sigma–Aldrich, bafilomycin A1 (Baf A1) was from Sangon Biotech (Shanghai, China). bay11-7082 was from Absin Inc. (Shanghai, China), antibody for Sca-1 and beads were all from BD Biosciences (San Jose, CA, USA). Caspase3 activity detection kit was from KeyGEN Biotech.