Adult butterflies were pinned with the mutant side up (ventral side up for adults with mutant clones on both surfaces). Fullmount photographs of butterflies were taken either on a Nikon D5300 digital camera mounted with an AF-S VR MicroNikkor 105 mm f/2.8G lens and an 80-LED ring light, or on a Keyence VHX-5000 digital microscope at 50X on a VH-Z00T lens. The high resolution images of butterflies and the wing mounts of moths were taken at 100X, 300X or 600X, and 50X, respectively, on the Keyence VHX-5000 fitted with a VH-Z100T lens.
Vhx 5000
Manufactured by Keyence
Sourced in Japan, Germany, United States, United Kingdom, Belgium
The VHX-5000 is a digital microscope system that captures high-resolution images and videos. It features advanced optics and imaging capabilities for detailed observation and analysis of samples.
Automatically generated - may contain errors
Lab products found in correlation
Szx16, by Olympus (5 mentions)
Hm 525 cryostat, by Thermo Fisher Scientific (2 mentions)
Mayer s hematoxylin and eosin y solution, by Merck Group (2 mentions)
Vh z20r, by Keyence (2 mentions)
D5300, by Nikon (2 mentions)
Mzapo microscope, by Leica (2 mentions)
Dimension icon, by Bruker (2 mentions)
Tissue tek cryomold, by Sakura Finetek (2 mentions)
Lsm 780, by Zeiss (2 mentions)
Escalab 250xi, by Thermo Fisher Scientific (2 mentions)
Lsm 710, by Zeiss (2 mentions)
Cryostat microtome, by Sakura Finetek (1 mentions)
Histofine simple stain max po m, by Nichirei Biosciences (1 mentions)
Anti slow clone m8421, by Merck Group (1 mentions)
Anti fast clone m4276, by Merck Group (1 mentions)
Sz2 ilst, by Olympus (1 mentions)
Vega 3 xmu sem, by TESCAN (1 mentions)
Vhx s550e, by Keyence (1 mentions)
Analytical balance, by Sartorius (1 mentions)
X maxn, by Oxford Instruments (1 mentions)
168 protocols using vhx 5000
1
Imaging Pinned Butterfly Specimens
2
Microscopic Analysis of Dental Filling Materials
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Specimen polished surfaces (n = 90, three surfaces for each tooth) were first examined under a digital microscope VHX-5000 (KEYENCE, Osaka, Japan), and one image was captured for each specimen at 100× magnification. The micrographs were coded by an expert examiner who was not involved in the experiment, displaying the canal wall surface of both groups at the coronal, middle, and apical thirds, for blinded analysis using the VHX-5000 software (KEYENCE, Osaka, Japan) to measure the total area of the filling materials and of the internal and external voids in µm3 following a previous study [28 (link)] (Figure 2 ). After that, the percentages of voids were calculated and statistically analyzed.
3
Growth Dynamics of Waterfern Westfalia
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W. australiana was grown on 2% agar (Agar-Agar, danish, Carl Roth GmbH, Karlsruhe, Germany) with half-strength MS salts (Murashige and Skoog 1962) (link) under sterile conditions at 25 ± 1°C (white light of 85 μmol m -2 s -1 16 h day and 8 h night photoperiod). Frond proliferation was monitored using a digital microscope Keyence VHX-5000 (Keyence Deutschland GmbH, Neu-Isenburg) by capturing images at 30 min intervals during 45 hours. In order to demonstrate growth dynamic the video file was converted to time-lapse movie MP4 format.
For histological examinations, intact plants were fixed in FAA (Weigel and Glazebrook 2008) , infiltrated with Spurr Resin (Plano, Wetzlar, Germany) and polymerized at 70°C for 24 h. The serial block sectioning was performed using Semimikrotom Leica Ultracut UTC (Leica Mikrosysteme GmbH, Vienna, Austria); the semi-thin 1 µm tissue sections were stained with Methylenblau-Borax (Waldeck GmbH & Co. KG, Muenster) and the stack of 311 micro images was obtained using digital microscope Keyence VHX-5000 with 600× lens magnification (Keyence Deutschland GmbH, Neu-Isenburg) and Image J software (Schindelin et al. 2012 ). The creation of three-dimensional models from a set of images (3D-reconstruction) was performed using Amira-Avizo software (ThermoFisherScientific).
For histological examinations, intact plants were fixed in FAA (Weigel and Glazebrook 2008) , infiltrated with Spurr Resin (Plano, Wetzlar, Germany) and polymerized at 70°C for 24 h. The serial block sectioning was performed using Semimikrotom Leica Ultracut UTC (Leica Mikrosysteme GmbH, Vienna, Austria); the semi-thin 1 µm tissue sections were stained with Methylenblau-Borax (Waldeck GmbH & Co. KG, Muenster) and the stack of 311 micro images was obtained using digital microscope Keyence VHX-5000 with 600× lens magnification (Keyence Deutschland GmbH, Neu-Isenburg) and Image J software (Schindelin et al. 2012 ). The creation of three-dimensional models from a set of images (3D-reconstruction) was performed using Amira-Avizo software (ThermoFisherScientific).
4
Skeletal Muscle Fiber Typing in Piglets
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Longissimus thoracis muscles obtained from 40-day-old piglets were frozen in dry-ice acetone (−78°C), and cryosections were generated using a cryostat microtome (Sakura Finetek). To analyze the distribution of skeletal muscle fiber types, the sections were immunostained with anti-slow (clone M8421, 1:400; Sigma) and anti-fast (clone M4276, 1:200; Sigma) myosin heavy-chain monoclonal antibodies, which are specific markers of type I and type II myofibers, respectively. Histofine Simple Stain MAX PO (M) (Nichirei) was used as the secondary antibody. The proportion of each fiber type in each section of the longissimus thoracis muscle was determined using a digital microscope (VHX-5000; Keyence).
5
Taxonomic Study of Insect Specimens
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The specimens examined are deposited in Chongqing Normal University, Chongqing, China (CN; CQNU NHM AMNH YNAU
6
Microstructural Analysis of Coral Skeletons
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For structural analyses and µCT scans of the live‐collected corals, two coral specimens (one H. cochlea and one H. aequicostatus, see Table 1 ) were fixed in 40 ml of 99.8% Ethanol. Afterward they were treated with 90% H2O2 for 48 hours to remove organic matter and dried for 12 h at 40°C. A stand‐alone micro‐CT (µCT) scanner (Skyscan 1172) was used at 180 KeV to study the 3D structure of the whole carbonate skeleton. The µCT scans provide a nominal resolution of 5–8 µm per voxel, depending on magnification scale, and were scanned at angular increments of 0.9° rotation steps over a period of 3–11 h.
The coral skeletons were then embedded in epoxy and sliced through the center along their long edge for SEM analysis. The sections were polished to approximately 35 µm thickness and gold sputtered. SEM images of the thin sections were taken with a Tescan® Vega 3 XMU SEM at 20 kV (SE detector). Backscattered electron (BSE) and secondary electron (SE) images were taken at 15 kV in low‐vacuum mode.
Light microscopy was undertaken on thin sections cut from the skeletal remains collected from the sediment with a Keyence VHX‐ 5000 equipped with a VH‐Z20R lens a VHX‐ 5020 camera and XY‐Stage VHX‐S550E.
The coral skeletons were then embedded in epoxy and sliced through the center along their long edge for SEM analysis. The sections were polished to approximately 35 µm thickness and gold sputtered. SEM images of the thin sections were taken with a Tescan® Vega 3 XMU SEM at 20 kV (SE detector). Backscattered electron (BSE) and secondary electron (SE) images were taken at 15 kV in low‐vacuum mode.
Light microscopy was undertaken on thin sections cut from the skeletal remains collected from the sediment with a Keyence VHX‐ 5000 equipped with a VH‐Z20R lens a VHX‐ 5020 camera and XY‐Stage VHX‐S550E.
7
Long-term Artificial Plaque Stability
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The visual characterization of the artificial plaque model was performed with a digital 3D microscope VHX-5000 (Keyence, Osaka, Japan) using a magnification of 20× and 200×. Long-term stability of the artificial plaques was assessed by microscopic examination and by weight monitoring. For this purpose, freshly prepared plaques were weighed and then incubated in sterile phosphate-buffered saline (PBS) at 37 °C. Plaques were removed at regular intervals, and excess liquid was carefully blotted with a tissue, after which samples were re-weighed with an analytical balance (Sartorius, Göttingen, Germany) or examined microscopically. All experiments were performed in triplicates. The long-term stability experiment was performed for a duration of 49 days.
8
Termite Diversity Survey in Indonesia
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Specimens were collected from nine locations in Indonesia from 2017–2019: around Sumatra Island (Simeulue Island, Karimunbesar Island, and Batam Island); Java Island from West Java province (Gunung Sindur, Cipinang, Cibinong, Karadenan, Parungpanjang) and the Special Capital Region of Jakarta (Pondok Kelapa); and Papua Island (Asmat Regency). Termites were preserved in 70% ethanol. Observations and photographs of the specimens were made using a digital microscope (VHX-5000, Keyence Corp., Osaka, Japan). Picture tracing was conducted using Drawing Pad (XP Pen Deco 2, Shenzhen, China) in Autodesk Sketchbook version 8.7.1. A total of 228 specimens of termite soldiers were measured (66 C. curvignathus, 10 C. elisae, 84 C. gestroi, 63 C. sepangensis, and 5 C. kalshoveni specimens). The identification was undertaken by collecting many characteristics from each specimen and matching them with references [16 (link),22 ,23 ,24 ,25 ,26 ,50 ,51 ,52 ,53 ]. The Indonesian specimens were deposited at the Museum Zoologicum Bogoriense and Research Center for Biomaterials–Indonesian Institute of Sciences (LIPI), Cibinong, Indonesia. All Japan specimens belong to the Research Institute for Sustainable Humanosphere (RISH)–Kyoto University, Kyoto, Japan.
9
MALDI-MSI Sample Preparation Protocol
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Sample preparation for MALDI was performed following the protocol previously optimized (Bhandari et al., 2015 (link)). Briefly, fresh samples were embedded in 2% (w/v) gelatin solution in a cryo-mold, and then 20 μm thick sections were cut at –20°C using a cryomicrotome (HM525 cryostat, Thermo Scientific, Dreiech, Germany). The sections were transferred to a glass slide and kept at –80°C until the day of the analysis. Before the matrix application, optical images of the sections were captured using a digital microscope VHX-5000 (Keyence GmbH, Neu-Isenburg, Germany). DHB (30 mg mL–1) in acetone:water (50:50, v/v, 0,1% TFA) was chosen as a matrix for untargeted metabolomics purpose and sprayed using an automated pneumatic sprayer system (SMALDIPrep, TransMIT GmbH, Giessen, Germany) (Bouschen et al., 2010 (link)) to ensure uniform coating of tissue sections with the microcrystalline matrix. The size and uniformity of the deposited crystals were checked prior to AP-SMALDI MS imaging experiments. At least two biological replicates of each tissue (i.e., root and culm) were analyzed by MSI.
10
Characterization of Compositionally Graded WC/Co-Alloy Composites
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The cladded beads and composites were observed by a digital microscope (VHX-5000, KEYENCE, Osaka, Japan). Microstructural observations of the beads and composites were performed by the FE-SEM at the acceleration voltage of 15 kV. The chemical compositions of the compositionally graded WC/Co-alloy composites were identified by using an SEM energy-dispersive X-ray spectroscopy (SEM-EDS: X-MaxN, Oxford Instruments plc, Abingdon, UK). The WC/Co-alloy composites were analyzed by X-ray diffraction (XRD) with an X-ray diffractometer (RINT-2500, Rigaku Corporation, Tokyo, Japan) using Cu-Kα radiation under the operation condition for an accelerating voltage of 40 kV and a current of 200 mA. The diffraction angle 2θ was measured from 30° to 120° at a step of 0.01° with a scan speed of 1°/min. Vickers microhardness tests were carried out on the cross-section of the composites with a micro Vickers hardness testing machine (HM-220D, Mitutoyo Corporation, Kawasaki, Japan).
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