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4 protocols using trichloroacetic acid

1

Surimi-Based Antioxidant Evaluation

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Frozen silver carp surimi was purchased from Fujian Anjing Foods Co., Ltd. (Xiamen, China), and kept at −20 °C until used. Black tea powder (caffeine removed) was purchased from Damin Foodstuff (Zhangzhou) Co., Ltd. (Zhangzhou, China). Tapioca starch, phosphate, salt and glutamine transaminase (TG enzyme) were commercially available.
1,1-diphenyl-2-picrylhydrazyl (DPPH) was purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China); 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was purchased from Beijing Solarbio Technology Co., Ltd. (Beijing, China); sodium dihydrogen phosphate, dibasic sodium phosphate, trichloroacetic acid, hydrogen peroxide, ferrous sulfate and potassium persulfate were of analytical grade and purchased from Xilong Chemical Co., Ltd. (Guangzhou, China). Absolute ethanol was purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China); ferric chloride, potassium ferricyanide, and 3,5-Dinitrosalicylic acid were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China).
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2

Determining Formaldehyde Levels in Mouse Brain

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The brain (0.1 g) was homogenized in 0.5 ml of SDN lysis buffer immediately after the mouse was sacrificed. Next, 0.5 ml of 10% trichloroacetic acid (analytical purity, Xilong Chemical Co., Ltd., USA) was added prior to centrifugation (12,000 rpm, 4°C, 30 min). The supernatants (0.4 ml) were pipetted into 1.5-ml Eppendorf tubes and mixed with 0.5 ml acetonitrile (HPLC-grade purity, Fisher Scientific, USA) and 0.1 ml 2,4-dinitrophenylhydrazine (DNPH, analytic purity, Beijing Chemical Reagent Research Institute, China). Next, the samples were centrifuged (12,000 rpm, 4°C, 10 min), incubated in a 60°C water bath for 30 min, and centrifuged again (12,000 rpm, 4°C, 10 min). The formaldehyde levels were determined with HPLC as described by Su and coworkers [38 ].
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3

Microbial Production of Carboxymethylcellulase

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The organism was B. amyloliquefaciens NCU116 identified by our laboratory (Zeng et al. 2017 (link)). Corn flour, bran and soybean meal were purchased from Nanchang Jingke Co. (Nanchang, China). Pectin powder was purchased from Solarbio Science & Technology Co. (Beijing, China). Agar and soluble starch were from Beijing Aobox Biotechnology Co. (Beijing, China). Folin phenol was purchased from Lida Biotechnology Co. (Shanghai, China). Carboxymethyl cellulase sodium was obtained from Sinopharm Chemical Reagent Co. (Shanghai, China). Casein was from Damao Chemical Reagent Co. (Tianjin, China). Na2HPO4·12H2O, KH2PO4, NaCl, Na2CO3, NaOH, trichloroacetic acid, and glucose purchased from Xilong Chemical Co. (Guangzhou, China) were of analytical grade.
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4

Reagents and Microbial Strains for Biochemical Assays

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Concentrated sulfuric acid, phenol, and anhydrous ethanol (C2H5OH, AR) were purchased from Sinopharm Chemical Reagent Co., Ltd. glucose standards, glucose (AR), Coomassie Brilliant Blue, bovine serum albumen (BSA), D(+) lacturonic acid, M - hydroxybenzidine, monosaccharide standard, PMP derivative reagent, KBr (AR), salicylicacid (AR), Tris (AR), bile salts, potassium hexacyanoferrate (III; K3[Fe(CN)6]; AR), purchased from Shanghai YuanYe Biotechnology Co., Ltd. Acetonitrile was purchased from Thermo Fisher Scientific Co., Ltd. (China). DNS (AR) was purchased from Guangzhou Testing Technology Co., Ltd. DPPH (AR) was purchased from Aladdin. Ascorbic acid (AR) Tianjin Kemiou Chemical Reagent Co. Ltd. FeSO4•7H2O (AR), 30% H2O2 (AR), K2(SO4)2 (AR), Trichloroacetic acid (AR), FeCl3 (AR) Xilong Chemical Co. Ltd. MRS medium and MRS medium without glucose Guangdong Haibo Biological Technology Co., Ltd. Inulin China Eclipse Moon Health Technology Co., Ltd. Lactobacillus casei was purchased from Guangdong Province General Microbiological Culture Collection Center. Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were purchased from the Guangdong Huankai Microbiology Technology co., Ltd. MH medium and NB medium were purchased from Haibo Biotechnology Co., Ltd. Dye resazurin Shanghai Haibo Biotechnology Co., Ltd.
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