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Artistic brush

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The Artistic-brush is a laboratory instrument designed for the precise and efficient application of various materials, such as liquids or powders, onto a surface. It features a brush-like applicator that can be used to apply samples in a controlled and uniform manner. The core function of the Artistic-brush is to facilitate the controlled deposition of materials for various laboratory applications.

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Lab products found in correlation

Cm3050, by Leica (2 mentions) Indium tin oxide coated glass slide, by Bruker (2 mentions) Fleximaging 4, by Bruker (2 mentions) Maldi ltq xl, by Thermo Fisher Scientific (2 mentions) Ultraflextreme maldi tof tof mass spectrometer, by Thermo Fisher Scientific (2 mentions) Imagequest 1, by Thermo Fisher Scientific (2 mentions)

2 protocols using artistic brush

1

MALDI Imaging of Brain Sections

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Matrix-assisted laser desorption/ionization (MALDI) imaging analyses were performed as described previously66 ,67 . Briefly, thin sections (8 μm) of the brain sections were prepared with a cryomicrotome (CM3050, Leica). Sections were attached onto indium tin oxide–coated glass slides (Bruker Daltonics) and were coated with 9-aminoacridine as the matrix (10 mg/mL, dissolved in 80% ethanol) by manually spraying with an artistic-brush (Mr. Hobby). The matrix was simultaneously applied to multiple sections compared in order to maintain consistent analyte extraction and co-crystallization conditions. MALDI imaging was performed using an Ultraflextreme MALDI-TOF/TOF mass spectrometer equipped with an Nd:YAG laser and a linear ion trap MS with a MALDI source (MALDI LTQ XL, Thermo Fisher Scientific) equipped with a nitrogen laser (337 nm; 60 Hz). Data were acquired in the negative reflectron mode with raster scanning at a pitch distance of 50 μm. For the TOF/TOF measurement, signals between m/z 50 and 1000 were collected and image reconstruction for both procedures was performed using FlexImaging 4.1 software (Bruker Daltonics). For the ion-trap instrument, the obtained spectral data were transformed to image data using ImageQuest 1.0.1 software (Thermo Fisher Scientific).
Kofuji S., Hirayama A., Eberhardt A.O., Kawaguchi R., Sugiura Y., Sampetrean O., Ikeda Y., Warren M., Sakamoto N., Kitahara S., Yoshino H., Yamashita D., Sumita K., Wolfe K., Lange L., Ikeda S., Shimada H., Minami N., Malhotra A., Morioka S., Ban Y., Asano M., Flanary V.L., Ramkissoon A., Chow L.M., Kiyokawa J., Mashimo T., Lucey G., Mareninov S., Ozawa T., Onishi N., Okumura K., Terakawa J., Daikoku T., Wise-Draper T., Majd N., Kofuji K., Sasaki M., Mori M., Kanemura Y., Smith E.P., Anastasiou D., Wakimoto H., Holland E.C., Yong W.H., Horbinski C., Nakano I., DeBerardinis R.J., Bachoo R.M., Mischel P.S., Yasui W., Suematsu M., Saya H., Soga T., Grummt I., Bierhoff H, & Sasaki A.T. (2019). IMP dehydrogenase-2 drives aberrant nucleolar activity and promotes tumorigenesis in glioblastoma. Nature cell biology, 21(8), 1003-1014.
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2

MALDI Imaging of Brain Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Matrix-assisted laser desorption/ionization (MALDI) imaging analyses were performed as described previously66 ,67 . Briefly, thin sections (8 μm) of the brain sections were prepared with a cryomicrotome (CM3050, Leica). Sections were attached onto indium tin oxide–coated glass slides (Bruker Daltonics) and were coated with 9-aminoacridine as the matrix (10 mg/mL, dissolved in 80% ethanol) by manually spraying with an artistic-brush (Mr. Hobby). The matrix was simultaneously applied to multiple sections compared in order to maintain consistent analyte extraction and co-crystallization conditions. MALDI imaging was performed using an Ultraflextreme MALDI-TOF/TOF mass spectrometer equipped with an Nd:YAG laser and a linear ion trap MS with a MALDI source (MALDI LTQ XL, Thermo Fisher Scientific) equipped with a nitrogen laser (337 nm; 60 Hz). Data were acquired in the negative reflectron mode with raster scanning at a pitch distance of 50 μm. For the TOF/TOF measurement, signals between m/z 50 and 1000 were collected and image reconstruction for both procedures was performed using FlexImaging 4.1 software (Bruker Daltonics). For the ion-trap instrument, the obtained spectral data were transformed to image data using ImageQuest 1.0.1 software (Thermo Fisher Scientific).
Kofuji S., Hirayama A., Eberhardt A.O., Kawaguchi R., Sugiura Y., Sampetrean O., Ikeda Y., Warren M., Sakamoto N., Kitahara S., Yoshino H., Yamashita D., Sumita K., Wolfe K., Lange L., Ikeda S., Shimada H., Minami N., Malhotra A., Morioka S., Ban Y., Asano M., Flanary V.L., Ramkissoon A., Chow L.M., Kiyokawa J., Mashimo T., Lucey G., Mareninov S., Ozawa T., Onishi N., Okumura K., Terakawa J., Daikoku T., Wise-Draper T., Majd N., Kofuji K., Sasaki M., Mori M., Kanemura Y., Smith E.P., Anastasiou D., Wakimoto H., Holland E.C., Yong W.H., Horbinski C., Nakano I., DeBerardinis R.J., Bachoo R.M., Mischel P.S., Yasui W., Suematsu M., Saya H., Soga T., Grummt I., Bierhoff H, & Sasaki A.T. (2019). IMP dehydrogenase-2 drives aberrant nucleolar activity and promotes tumorigenesis in glioblastoma. Nature cell biology, 21(8), 1003-1014.
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