Mf 2012

Manufactured by Bioanalytical Systems

Sourced in United States

The MF-2012 is a laboratory instrument designed for the analytical analysis of various samples. It is a versatile and reliable tool for researchers and scientists in the field of bioanalysis. The core function of the MF-2012 is to perform precise and accurate measurements of chemical and biological components within a sample.

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Lab products found in correlation

Mf 2052, by Bioanalytical Systems (3 mentions) Mw 1032, by Bioanalytical Systems (2 mentions) Chi 660b, by CH Instruments (1 mentions) Agilent 1200 series hplc system, by Agilent Technologies (1 mentions) Palmsens3 potentiostat galvanostat, by PalmSens (1 mentions) Zive sp1, by Wonatech (1 mentions) Jsm 661olv, by JEOL (1 mentions) Mw 4130, by Bioanalytical Systems (1 mentions) K3fe cn 6, by Merck Group (1 mentions) Autolab potentiostat galvanostat, by Metrohm (1 mentions) Sp 200 potentiostat, by Bio-Logic (1 mentions)

11 protocols using mf 2012

Electrochemical Characterization of GCE and AGCE

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The interface 1000 Gamry electrochemical
workstation was utilized in all electrochemical measurements. Such
measurements were done using a three-electrode system. The working
electrode was a bare GCE model MF-2012, 3.0 mm in diameter (Bioanalytical
Systems), or an AGCE. Saturated double-junction Ag/AgCl and platinum
wire were used as reference and counter electrodes, respectively.
All experiments were performed at 25 ± 1 °C. DPV was performed
with a pulse amplitude of 50 mV, a pulse width of 50 ms, and a pulse
time of 200 ms. The scan rate was 50 mV/s.

Abdel-Aziz A.M., Hassan H.H, & Badr I.H. (2022). Activated Glassy Carbon Electrode as an Electrochemical Sensing Platform for the Determination of 4-Nitrophenol and Dopamine in Real Samples. ACS Omega, 7(38), 34127-34135.

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Electrochemical Analysis of EMn in Phosphate Buffer

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Electrochemical measurements of EMn were made in 0.1 M of phosphate buffer solutions adjusted to a pH comprised between 4 and 1 using HCl. KCl 0.1 M was added to the phosphate buffer solutions to maintain the ionic strength constant. A 3-electrode cell configuration was used. The working electrode was a glassy carbon electrode from Bioanalytical Systems Inc. (West Lafayette, IN, USA, model MF-2012; 3 mm in diameter) and the counter electrode was a platinum wire. All potential values were referred to the SCE system. A bipotentiostat from CH Instruments (model 920C) was used.

Francezon N., Herbaut M., Bardeau J.F., Cougnon C., Bélanger W., Tremblay R., Jacquette B., Dittmer J., Pouvreau J.B., Mouget J.L, & Pasetto P. (2021). Electrochromic Properties and Electrochemical Behavior of Marennine, a Bioactive Blue-Green Pigment Produced by the Marine Diatom Haslea ostrearia. Marine Drugs, 19(4), 231.

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Electrochemical and HPLC Analysis of Sudan 1

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Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) measurements were carried out with a PalmSens3 potentiostat/galvanostat (PalmSens BV, Houten, The Netherlands) and CHI 660B (CH Instruments, Inc., Austin, TX, USA). A conventional three-electrode system, consisting of glassy carbon working electrode (MF-2012, 3.0 mm diameter; BASi, West Lafayette, IN, USA), Ag/AgCl reference electrode (MF- -2052; BASi) and platinum wire auxiliary electrode (MW-1032; BASi), was used. Moreover, Agilent 1200 series HPLC system (Agilent Technologies, Inc., Santa Clara, CA, USA) was used for Sudan 1 chromatographic analysis on the ACE C18 (250 mm×4.6 mm) column (Advanced Chromatography Technologies Ltd, Aberdeen, UK), with a mobile phase consisting of methanol/acetonitrile (40:60, by volume) and a constant flow rate of 0.5 mL/min. The injection volume was 10 μL and the column temperature was set at 25 °C. Finally, the wavelength was set at 254 nm for quantitative analysis.

Karaboduk K, & Hasdemır E. (2018). Voltammetric Determination of Sudan 1 in Food Samples Using Its Cu(II) Compound. Food Technology and Biotechnology, 56(4), 573-580.

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Electrochemical Characterization of Samples

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Electrochemical measurements were carried out using an electrochemical workstation (ZIVE SP1, WonATech, Seoul, Korea). Standard three-electrode cell (working electrode: sample, counter electrode: platinum. In addition, reference electrode: saturated Ag/AgCl) was used. To make a working electrode, a colloidal solution of the sample in an ethanolic solution of Nafion was taken, which is coated on a glassy carbon electrode (d = 3 mm, MF-2012, BASI, West Lafayette, IN, USA). Aqueous sodium sulfate (0.2 M) solution was used as an electrolyte.

Shrestha S., Sapkota K.P., Lee I., Islam M.A., Pandey A., Gyawali N., Akter J., Mohan H., Shin T., Jeong S, & Hahn J.R. (2022). Carbon-Based Ternary Nanocomposite: Bullet Type ZnO–SWCNT–CuO for Substantial Solar-Driven Photocatalytic Decomposition of Aqueous Organic Contaminants. Molecules, 27(24), 8812.

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Electrochemical Characterization of Fe3O4@Graphene

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All electrochemical measurements were performed using a potentiostat/galvanostat Autolab PGSTAT 128N with NOVA software and typical three-electrode 8 mL cell equipment. Glassy carbon electrode (GCE, diameter 3 mm, produced by BASi, MF-2012 model), modified or unmodified, was adopted as a working electrode, silver/silver chloride electrode was used as a reference electrode, and platinum wire as a counter electrode. The visualization and characterization of nanomaterials based on Fe₃O₄@graphene were carried out using a scanning electron microscope (SEM, model JEOL JSM-661OLV) at 16 kV of beam voltage. The nanocomposite was applied directly to the microscope aluminum stubs covered with carbon tape, without sputtering. Observed measurements were averaged.

Baluta S., Romaniec M., Halicka-Stępień K., Alicka M., Pieła A., Pala K, & Cabaj J. (2023). A Novel Strategy for Selective Thyroid Hormone Determination Based on an Electrochemical Biosensor with Graphene Nanocomposite. Sensors (Basel, Switzerland), 23(2), 602.

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Electrochemical Characterization of Potassium Ferricyanide

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All experiments were performed on a volume of 50 mL on a electrochemical cell (height = 35 mm, diameter = 60 mm) of potassium ferricyanide

K_{3} {[Fe (CN)}_{6}]

; this analyte is common to use to test potentiostats [12 ,30 (link),31 ,32 ], since its kinetics is well known and it describes an electrochemical reversible behavior [23 ,33 ,34 ]. Ferricyanide can be reduced to ferrocyanide as Equation (2) shows; the backward direction of the reaction corresponds to the ferrocyanide oxidation to ferricyanide as Equation (3) describes.

Fe {(CN)}_{6}^{3 -} {+ e}^{-} \to Fe {(CN)}_{6}^{4 -}

Fe {(CN)}_{6}^{4 -} \to Fe {(CN)}_{6}^{3 -} {+ e}^{-}

In the experiments two analyte concentrations of 1 mM and 10 mM of K₃[Fe(CN)₆] (Sigma-Aldrich, Saint Louis, MO, USA, CAS: 13746-66-2) were used to evaluate the EPS. The electrolyte support used was 0.5 M KCl (Fermont, presentation no. 24842). The reference electrode (RE) used is Ag/AgCl (BASi model MF-2052). A platinum wire (BASi model MW-4130) and a disk glassy carbon electrode (BASi model MF-2012, diameter ϕ = 3 mm) was used as the Counter Electrode (CE) and the Working Electrode (WE), respectively, as it is shown in Figure 5B.

Muñoz-Martínez A.I., González Peña O.I., Colomer-Farrarons J., Rodríguez-Delgado J.M., Ávila-Ortega A, & Dieck-Assad G. (2018). Electrochemical Instrumentation of an Embedded Potentiostat System (EPS) for a Programmable-System-On-a-Chip. Sensors (Basel, Switzerland), 18(12), 4490.

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Electrochemical Characterization of NB-Ni Peptide

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All electrochemical experiments were carried out in an anaerobic atmosphere continuously purging nitrogen gas though the solution and headspace of the electrochemical cell. The CV experiments were set up using a Bio-Logic potentiostat (EC50) and a three-electrode configuration with a Ag/AgCl electrode (BASi) as a reference and a platinum wire as a counter electrode. A glassy carbon electrode (3 mm, MF-2012, BASi) was used as a working electrode, and it was polished with (1 μM) alumina slurry before every measurement. For every experiment, 5 ml of NB-Ni peptide at various concentrations was filled into a three-port Echem cell and connected to the electrodes while purging the solution with N₂. During the CV scan, the purging needle was withdrawn from the solution while still purging the headspace. Data were acquired using the EC-lab software (V10.44) and then baseline-corrected and analyzed using MATLAB. All potentials quoted in this work were referenced to an SHE and calculated as E(SHE) = E(Ag/AgCl) + 205 mV.

Timm J., Pike D.H., Mancini J.A., Tyryshkin A.M., Poudel S., Siess J.A., Molinaro P.M., McCann J.J., Waldie K.M., Koder R.L., Falkowski P.G, & Nanda V. (2023). Design of a minimal di-nickel hydrogenase peptide. Science Advances, 9(10), eabq1990.

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Electrochemical Characterization of Copper Electrolyte

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All electrochemical measurements were performed inside a glovebox in a nitrogen atmosphere. Anhydrous DMSO was used as the solvent. A 3-electrode glass cell consisting of a glassy carbon working electrode (BASi, MF-2012), copper foil counter and reference electrodes, and copper(I) trifluoromethanesulfonate was used for the measurements. Electrochemical data was collected on a multi-channel potentiostat (AMETEK Scientific Instruments, VersaSTAT 4). Voltammograms were recorded between −0.3 and 0.8 V vs Cu⁺/Cu at a scan rate of 100 mV s⁻¹.

Nazarova A.L., Zayat B., Fokin V.V, & Narayan S.R. (2022). Electrochemical Studies of the Cycloaddition Activity of Bismuth(III) Acetylides Towards Organic Azides Under Copper(I)-Catalyzed Conditions. Frontiers in Chemistry, 10, 830237.

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Voltammetric Detection of Explosive Precursors

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Voltammetric experiments were carried out
with a Metrohm Autolab potentiostat/galvanostat (PGSTAT204, Switzerland)
and controlled by Nova 2.1.5 software. In addition, the GC electrode
(BASI MF-2012), Ag/AgCl electrode (BASI MF-2052), and platinum (Pt)
wire electrode were used as the working electrode, reference electrode,
and auxiliary electrode, respectively.
The developed method
was validated against GC/MS using a Thermo Scientific Trace gas chromatography
coupled with a quadrupole analyzer and a DSQII MS containing electron
impact ionization for TATP and a UV–vis spectrophotometer (Shimadzu
UV-1800) for HMTD. The surface analysis of the developed MIP sensor
electrode was carried out with an FEI Model Quanta 450 FEG Scanning
Electron Microscopy (SEM) instrument.

Sağlam Ş., Üzer A, & Apak R. (2022). Direct Determination of Peroxide Explosives on Polycarbazole/Gold Nanoparticle-Modified Glassy Carbon Sensor Electrodes Imprinted for Molecular Recognition of TATP and HMTD. Analytical Chemistry, 94(50), 17662-17669.

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Electrochemical Analysis of Organometallic Complexes

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CV was carried out with an EC Epsilon potentiostat (C-3 cell stand) purchased from BASi Analytical Instruments (West Lafayette, IN). A glassy carbon (GC) electrode from BASi (MF-2012), 3 mm in diameter, was polished on a white nylon pad (BASi MF-2058) with different-sized diamond polishes (15, 6, and 1 μm) to ensure a mirrorlike finish. Between each measurement, the GC electrode was polished with three diamond polishes. A three-electrode cell configuration was used with GC as the working electrode, a silver wire (0.5 mm diameter) quasi-reference electrode housed in a glass tube (7.5 cm × 5.7 mm) with a porous CoralPor tip, and a platinum wire (7.5 cm) as the counter electrode (BASi MW-1032). All solutions were bubbled with N₂ gas for at least 15 min prior to experimentation and kept under a humidified N₂ gas blanket. All potentials herein are reported versus Fc/Fc⁺ (E_½ = 0.0 V). For each electrochemical analysis, 3.0 mg of complex was dissolved in 3.0 mL of anhydrous DMF containing 0.1 M tetrabutylammonium perchlorate as the supporting electrolyte.

Johnston H.M., Pota K., Barnett M.M., Kinsinger O., Braden P., Schwartz T.M., Hoffer E., Sadagopan N., Nguyen N., Yu Y., Gonzalez P., Tircsó G., Wu H., Akkaraju G., Chumley M.J, & Green K.N. (2019). Enhancement of the Antioxidant Activity and Neurotherapeutic Features through Pyridol Addition to Tetraazamacrocyclic Molecules. Inorganic chemistry, 58(24), 16771-16784.

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