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Integrin α4β7

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Integrin α4β7 is a cell surface receptor that mediates adhesion and migration of lymphocytes. It is expressed on subsets of T cells and plays a role in the homing of lymphocytes to mucosal tissues.

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Lab products found in correlation

Efluor 660, by Thermo Fisher Scientific (2 mentions) Ctla 4 pe, by BD (2 mentions) Brilliant violet 421, by BioLegend (2 mentions) Apc cy7, by BD (2 mentions) I a 1 e, by BD (2 mentions) Ex527, by Bio-Techne (2 mentions) Apc cy7, by BioLegend (2 mentions) F4 80, by BioLegend (2 mentions) Cd103, by BioLegend (2 mentions)

2 protocols using integrin α4β7

1

Flow Cytometry Analysis of Murine Immune Cells

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For flow cytometry, we purchased mAbs to murine CD4 (Pacific Blue and APC-H7 from Biolegend, PE-CF594 and APC-Cy7 from BD Pharmingen), Ki-67 (PerCP-Cy5.5, clone B56, BD Pharmingen), Foxp3 (PE and eFluor450, clone FJK-16s, eBioscience), CTLA4 (PE, BD Pharmingen), I-A/I-E (FITC, BD Pharmingen), CD11b (APC from eBioscience, Pe/Cy7 from Biolegend), Ly-6G (APC, Biolegend), Ly-6C (Pacific Blue, Biolegend), Gr-1 (APC, Biolegend), F4/80 (APC and PE, Biolegend), CD11c (APC/Cy7, Biolegend), CD39 (eFluor 660, eBioscience), CD103 (PE, Biolegend), CD127 (Brilliant Violet421, Biolegend), CCR9 (FITC, Biolegend), and Integrin α4β7 (APC, Biolegend). For the analysis of mesenteric lymph nodes, we used the Aqua LIVE/DEAD® Fixable Dead Cell Stain Kit to exclude false positive signals from dead and apoptotic cells, and then washed two times prior to antibody staining. We purchased EX-527 from Tocris Bioscience. Drugs were dissolved in dimethylsulfoxide (DMSO), and DMSO was used as a control.
Akimova T., Xiao H., Liu Y., Bhatti T.R., Jiao J., Eruslanov E., Singhal S., Wang L., Han R., Zacharia K., Hancock W.W, & Beier U.H. (2014). Targeting Sirtuin-1 Alleviates Experimental Autoimmune Colitis by Induction of Foxp3+ T-Regulatory Cells: Sirt1 targeting alleviates autoimmune colitis. Mucosal immunology, 7(5), 1209-1220.
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2

Flow Cytometry Analysis of Murine Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometry, we purchased mAbs to murine CD4 (Pacific Blue and APC-H7 from Biolegend, PE-CF594 and APC-Cy7 from BD Pharmingen), Ki-67 (PerCP-Cy5.5, clone B56, BD Pharmingen), Foxp3 (PE and eFluor450, clone FJK-16s, eBioscience), CTLA4 (PE, BD Pharmingen), I-A/I-E (FITC, BD Pharmingen), CD11b (APC from eBioscience, Pe/Cy7 from Biolegend), Ly-6G (APC, Biolegend), Ly-6C (Pacific Blue, Biolegend), Gr-1 (APC, Biolegend), F4/80 (APC and PE, Biolegend), CD11c (APC/Cy7, Biolegend), CD39 (eFluor 660, eBioscience), CD103 (PE, Biolegend), CD127 (Brilliant Violet421, Biolegend), CCR9 (FITC, Biolegend), and Integrin α4β7 (APC, Biolegend). For the analysis of mesenteric lymph nodes, we used the Aqua LIVE/DEAD® Fixable Dead Cell Stain Kit to exclude false positive signals from dead and apoptotic cells, and then washed two times prior to antibody staining. We purchased EX-527 from Tocris Bioscience. Drugs were dissolved in dimethylsulfoxide (DMSO), and DMSO was used as a control.
Akimova T., Xiao H., Liu Y., Bhatti T.R., Jiao J., Eruslanov E., Singhal S., Wang L., Han R., Zacharia K., Hancock W.W, & Beier U.H. (2014). Targeting Sirtuin-1 Alleviates Experimental Autoimmune Colitis by Induction of Foxp3+ T-Regulatory Cells: Sirt1 targeting alleviates autoimmune colitis. Mucosal immunology, 7(5), 1209-1220.
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