Log In Sign up
The largest database of trusted experimental protocols

Rabbit anti tfam

Manufactured by Abcam
Visit Supplier
Sourced in United States

Rabbit anti-TFAM is a primary antibody that specifically binds to the Transcription Factor A, Mitochondrial (TFAM) protein. TFAM is a nuclear-encoded protein that plays a crucial role in the replication and transcription of mitochondrial DNA. This antibody can be used to detect and analyze the expression of TFAM in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti pgc 1α, by Abcam (2 mentions) Mouse anti sirt1, by Abcam (1 mentions) Rabbit anti cox 4, by Cell Signaling Technology (1 mentions) Dm6000m, by Leica camera (1 mentions) Rabbit anti tomm20, by Abcam (1 mentions) Nitrocellulose membrane, by Merck Group (1 mentions) Rabbit anti atp5a1, by ABclonal (1 mentions) Mouse anti β actin, by Santa Cruz Biotechnology (1 mentions) Mouse anti gapdh, by Santa Cruz Biotechnology (1 mentions) Rabbit anti lc3a b, by Cell Signaling Technology (1 mentions) Bn 34, by Cell Signaling Technology (1 mentions) Py701 stat1, by Cell Signaling Technology (1 mentions) Anti mouse biotin, by Merck Group (1 mentions) Rabbit anti biotin, by Cell Signaling Technology (1 mentions) Mouse anti mitochondria, by Abcam (1 mentions) Mouse anti mre11, by Abcam (1 mentions) Stat1, by Cell Signaling Technology (1 mentions) Mouse anti brdu, by BD (1 mentions) Laemmli buffer, by Carl Roth (1 mentions) Goat anti rabbit horseradish peroxidase hrp conjugated secondary antibody, by Bio-Rad (1 mentions)

5 protocols using rabbit anti tfam

1

Immunohistochemical Analysis of SIRT1, TFAM, COXIV, and TOMM20

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemical staining was performed on 5-μm sections of paraformaldehyde- fixed, paraffin-embedded tumor samples, adjacent nontumoral liver tissue, normal liver tissue and mouse lung tissue. The details of the immunohistochemistry were described previously [28 (link)]. The slides were incubated with mouse anti-SIRT1 (Abcam), rabbit anti-TFAM (Abcam), rabbit anti-COXIV (Cell Signaling Technology, Danvers, MA, USA) and rabbit anti-TOMM20 (Abcam) primary antibodies. The mouse lung tissue sections (5-μm thick) were stained with hematoxylin and eosin (H&E) so that tumor nodules could be observed. The slides were evaluated under a light microscope (Leica, DM6000M).
Li Y., Xu S., Li J., Zheng L., Feng M., Wang X., Han K., Pi H., Li M., Huang X., You N., Tian Y., Zuo G., Li H., Zhao H., Deng P., Yu Z., Zhou Z, & Liang P. (2016). SIRT1 facilitates hepatocellular carcinoma metastasis by promoting PGC-1α-mediated mitochondrial biogenesis. Oncotarget, 7(20), 29255-29274.
+ Open protocol
+ Expand
2

Immunoblot Analysis of Mitochondrial Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Excised TA muscles were dissolved in total protein extraction buffer (keyGEN Biotech, Nanjing, China) with protease and phosphatase inhibitors. The total protein (30 μg) was fractionated on 6–12% sodium dodecyl sulfate (SDS) polyacrylamide gels and transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA) by electroblotting. The membranes were blocked in Tris-buffered saline containing 5% milk and then incubated overnight at 4 °C with one of the following primary antibodies: rabbit anti-TFAM (Cat No: ab131607, Abcam, Cambridge, MA, USA), rabbit anti-PGC-1α (Cat No: 19142-1, Abcam), rabbit anti-PFKP (Cat No: 8164T, CST, Danvers, MA, USA), rabbit anti-phosphor-ACC (Cat No: 3661s, CST), rabbit anti-ACC (Cat No: 3662s, CST), rabbit anti-COX 1 (Cat No: A6564, ABclonal, Wuhan, Hubei, China), rabbit anti-NDUFS3 (Cat No: A8013, ABclonal), rabbit anti-SDHA (Cat No: A2594, ABclonal), rabbit anti-ATP5A1 (Cat No: A5884, ABclonal), rabbit anti-UQCRC2 (Cat No: A4184, ABclonal), mouse anti-GAPDH (Cat No: sc-32233, Santa Cruz, Felton, CA, USA) or mouse anti-β-actin (Cat No: sc-69879, Santa Cruz).
Xu D.Q., Li C.J., Jiang Z.Z., Wang L., Huang H.F., Li Z.J., Sun L.X., Fan S.S., Zhang L.Y, & Wang T. (2020). The hypoglycemic mechanism of catalpol involves increased AMPK-mediated mitochondrial biogenesis. Acta Pharmacologica Sinica, 41(6), 791-799.
+ Open protocol
+ Expand
3

Comprehensive Antibody Toolkit for Cellular Analyses

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in MIRA, immunofluorescence, and Western blot assays are as follows: mouse anti-biotin (Sigma-Aldrich, BN-34), rabbit anti-biotin (1:200; Cell Signaling Technology, D5A7), rabbit anti-TFAM (Abcam, ab131607), mouse anti-MRE11 (Abcam, ab214), rabbit anti-cGAS (Novus, NBP1-86761), mouse anti-mitochondria (Abcam, ab3298), pY701 STAT1 (Cell Signaling Technology, 9167), STAT1 (Cell Signaling Technology, 9176, 14995), mouse anti-DNA (EMD Millipore, CBL186), rabbit anti-LC3A/B (Cell Signaling Technology, D3U4C), mouse anti-oxphos (Abcam, ab3601), mouse anti-BrdU (BD Pharmingen, 555627), rabbit anti-pS366 STING (Cell Signaling Technology, 50907), rabbit anti-TMEM173 (STING; Abcam, ab227704), and mouse anti-RAD51C (Abnova, H00005889-M01).
Luzwick J.W., Dombi E., Boisvert R.A., Roy S., Park S., Kunnimalaiyaan S., Goffart S., Schindler D, & Schlacher K. (2021). MRE11-dependent instability in mitochondrial DNA fork protection activates a cGAS immune signaling pathway. Science Advances, 7(51), eabf9441.
+ Open protocol
+ Expand
4

Immunoblotting of T Cell Metabolism

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were prepared in RIPA buffer supplemented with a complete protease-inhibitor cocktail (Roche) and mixed in equal amounts with Laemmli buffer (Roth) before denaturation at 98 °C for 5 min. Protein quantification was performed with the Pierce 660 nm Protein Assay (Pierce). Protein samples were separated via SDS-PAGE, blotted onto nitrocellulose membranes, blocked with 5% BSA, and incubated with monoclonal rabbit anti-HIF-1α (Cell Signaling Technology, clone D1S7W). Detection was carried out with a goat anti-rabbit horse radish peroxidase (HRP) conjugated secondary antibody (BioRad), visualized with the enhanced chemiluminescent SuperSignal reagent (Pierce). For the detection of PGC-1α, TFAM, and elector transport chain (ETC) complex expression, Tpex and Tex cells were FACS sorted from LCMVCL13 infected mice 30 d.p.i. and 5x105 T cells were directly lysed in RIPA buffer. Detection of PGC-1α, TFAM, and ETC complexes was carried out using rabbit anti-PGC-1α (Abcam), rabbit-anti-TFAM (Abcam), and an OxPhos rodent antibody cocktail (Thermo) with goat anti-mouse or rabbit HRP-conjugated secondary antibodies (both BioRad).
Wu H., Zhao X., Hochrein S.M., Eckstein M., Gubert G.F., Knöpper K., Mansilla A.M., Öner A., Doucet-Ladevèze R., Schmitz W., Ghesquière B., Theurich S., Dudek J., Gasteiger G., Zernecke A., Kobold S., Kastenmüller W, & Vaeth M. (2023). Mitochondrial dysfunction promotes the transition of precursor to terminally exhausted T cells through HIF-1α-mediated glycolytic reprogramming. Nature Communications, 14, 6858.
+ Open protocol
+ Expand
5

Mitochondrial Protein Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were harvested by digestion with Trypsin. After centrifugation at 1000 rpm, cells were resuspended in ice-cold lysis buffer (Beyotime Biotechnology, Cat# P0013C) for 30 min on ice. Lysates were centrifugated at 10,000g for 10 min and the supernatant was collected. Western blot analysis was performed using standard protocols, and the following commercial antibodies were used: rabbit anti-TFAM (Abcam, Cat# 176558), rabbit anti-beta Actin (Proteintech, Cat# 20536-1-AP), rabbit anti-TOMM20 (Abclonal, Cat# A6774), and rabbit anti-TIMM23 (Abclonal, Cat# A8688), mouse anti-α-Tubulin (Abclonal, Cat# AC012).
Lv J., Yi Y., Qi Y., Yan C., Jin W., Meng L., Zhang D, & Jiang W. (2022). Mitochondrial homeostasis regulates definitive endoderm differentiation of human pluripotent stem cells. Cell Death Discovery, 8, 69.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!