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Sihif1 α

Manufactured by Thermo Fisher Scientific

SiHIF1−α is a laboratory product manufactured by Thermo Fisher Scientific. It is a recombinant protein that contains the hypoxia-inducible factor 1-alpha (HIF1-α) domain. HIF1-α is a transcription factor that plays a key role in the cellular response to hypoxia.

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2 protocols using sihif1 α

1

Silencing Hypoxia-Inducible Factors

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About 1x106 cells were washed and resuspended in 100μl Amaxa nucleofection buffer (Lonza). 100nM of the following siRNAs were then added to the mixtures: siARG2 (Ambion, #s1571), siARG1 (Ambion, #s1568), siHIF1−α (Ambion, #s6539), siHIF2−α (Ambion, #s4700), siARNT (Ambion, #s1613), and control siRNA (Ambion, silencer select control siRNA #1). The mixtures were electroporated using the preset K562 ATCC program on the Amaxa nucleofection device (Lonza). Cells were resuspended in 5ml of RPMI media for recovery before further treatments.
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2

Knockdown of HIF-1α in Cell Lines

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Cells were seeded at a density to reach 30–50% confluence by the day of transfection. Cells were transfected as described previously [45 (link)] with small interfering RNA (siRNA) against HIF-1α (siHIF-1α) (Ambion/Life Technologies) using Oligofectamine (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. Non-targeting siRNA, were used as a negative control. Optimal knockdown was verified by western blot.
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