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Sihif1 α

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SiHIF1−α is a laboratory product manufactured by Thermo Fisher Scientific. It is a recombinant protein that contains the hypoxia-inducible factor 1-alpha (HIF1-α) domain. HIF1-α is a transcription factor that plays a key role in the cellular response to hypoxia.

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Lab products found in correlation

Control sirna, by Thermo Fisher Scientific (1 mentions) Amaxa nucleofection device, by Lonza (1 mentions) Sihif2 α, by Thermo Fisher Scientific (1 mentions) Oligofectamine, by Thermo Fisher Scientific (1 mentions)

2 protocols using sihif1 α

1

Silencing Hypoxia-Inducible Factors

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About 1x106 cells were washed and resuspended in 100μl Amaxa nucleofection buffer (Lonza). 100nM of the following siRNAs were then added to the mixtures: siARG2 (Ambion, #s1571), siARG1 (Ambion, #s1568), siHIF1−α (Ambion, #s6539), siHIF2−α (Ambion, #s4700), siARNT (Ambion, #s1613), and control siRNA (Ambion, silencer select control siRNA #1). The mixtures were electroporated using the preset K562 ATCC program on the Amaxa nucleofection device (Lonza). Cells were resuspended in 5ml of RPMI media for recovery before further treatments.
Ng K.P., Manjeri A., Lee L.M., Chan Z.E., Tan C.Y., Tan Q.D., Majeed A., Lee K.L., Chuah C., Suda T, & Ong S.T. (2018). The arginase inhibitor Nω−hydroxy−nor−arginine (nor−NOHA) induces apoptosis in leukemic cells specifically under hypoxic conditions but CRISPR/Cas9 excludes arginase 2 (ARG2) as the functional target. PLoS ONE, 13(10), e0205254.
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2

Knockdown of HIF-1α in Cell Lines

Cited 1 time
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Cells were seeded at a density to reach 30–50% confluence by the day of transfection. Cells were transfected as described previously [45 (link)] with small interfering RNA (siRNA) against HIF-1α (siHIF-1α) (Ambion/Life Technologies) using Oligofectamine (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. Non-targeting siRNA, were used as a negative control. Optimal knockdown was verified by western blot.
Alshaker H., Wang Q., Kawano Y., Arafat T., Böhler T., Winkler M., Cooper C, & Pchejetski D. (2016). Everolimus (RAD001) sensitizes prostate cancer cells to docetaxel by down-regulation of HIF-1α and sphingosine kinase 1. Oncotarget, 7(49), 80943-80956.
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