Sigmacote siliconizing reagent

Manufactured by Merck Group

Sigmacote is a siliconizing reagent manufactured by the Merck Group. It is designed to provide a thin, uniform, and durable silicone coating on laboratory glassware and other surfaces. The core function of Sigmacote is to create a hydrophobic surface that helps prevent the adhesion of various biological samples and materials.

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Lab products found in correlation

Anti human cd61, by BD (2 mentions) Collagen type 1 chrono par aggregation reagent, by Chrono-Log (2 mentions) D dimer elisa kit, by Abcam (2 mentions) Alexa fluor 647 conjugated human fibrinogen, by Thermo Fisher Scientific (2 mentions) Sylgard 184 silicone elastomer kit, by Dow (2 mentions) Enzygnost f1 2 monoclonal kit, by Siemens (2 mentions) Fumaric acid, by Merck Group (1 mentions) Zirconium oxychloride octahydrate, by Merck Group (1 mentions) Dade innovin prothrombin time reagent, by Siemens (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Elisa kit, by MyBioSource (1 mentions) Dade innovin pt reagent, by Siemens (1 mentions) Corn trypsin inhibitor cti, by Prolytix (1 mentions)

3 protocols using sigmacote siliconizing reagent

Zirconium Oxychloride-based Synthesis

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Zirconium oxychloride octahydrate (ZrOCl₂·8H₂O, purity ≥99.5%), fumaric acid,
and Sigmacote siliconizing reagent were obtained from Sigma-Aldrich
Co. N,N-Dimethylformamide (DMF),
formic acid (purity ≥99.0%), and anhydrous methanol were obtained
from EMD Millipore Chemicals. Anhydrous acetone was obtained from
Acros Organics.

Fuchs A., Knechtel F., Wang H., Ji Z., Wuttke S., Yaghi O.M, & Ploetz E. (2023). Water Harvesting at the Single-Crystal Level. Journal of the American Chemical Society, 145(26), 14324-14334.

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Platelet Aggregation and Coagulation Assay

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The following reagents were obtained: anti-human CD61 (BD Biosciences, San Jose, CA), Alexa Fluor647-conjugated human fibrinogen (Life Technologies, Grand Island, NY), collagen type I Chrono-Par aggregation reagent (Chrono-log, Havertown, PA), CTI (corn trypsin inhibitor; Hematologic Technologies, Essex Junction, VT), Phe-Pro-Arg-chloromethylketone (PPACK; Hematologic Technologies, Essex Junction, VT), Dade Innovin prothrombin time reagent (Siemens, Malvern, PA), Enzygnost F1+2 monoclonal kit (Siemens Healthcare Diagnostic, Tarrytown, NY), D-dimer ELISA Kit (Abcam, Cambridge, MA), human FPA (fibrinopeptide-A), ELISA Kit (MyBioSource, San Diego, CA), EDTA (Sigma, St. Louis, MO), H-Gly-Pro-Arg-Pro-OH (EMD Chemicals, San Diego, CA), fluorescein Phe-Pro-Arg-chloromethylketone (fluorescein-PPACK; Hematologic Technologies, Essex Junction, VT), Sigmacote siliconizing reagent (Sigma, St. Louis, MO), and Sylgard 184 Silicone Elastomer kit (Dow Corning, Auburn, MI). O1A6 FXI antibody was a gift from Dr Andras Gruber (Department of Biomedical Engineering, Oregon Health and Science University). See the online-only Data Supplement for catalog numbers.

Zhu S., Chen J, & Diamond S.L. (2018). Establishing the Transient Mass Balance of Thrombosis: From Tissue Factor to Thrombin to Fibrin Under Venous Flow. Arteriosclerosis, Thrombosis, and Vascular Biology, 38(7), 1528-1536.

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Platelet and Coagulation Assays

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The following reagents were obtained: Anti-human CD61 (BD Biosciences, San Jose, CA), Alexa Fluor®647 conjugated human fibrinogen (Life Technologies, Grand Island, NY), Collagen Type I Chrono-Par™ aggregation reagent (Chrono-log, Havertown, PA), corn trypsin inhibitor (CTI, Haematologic Technologies, Essex Junction, VT), Phe-Pro-Arg-chloromethylketone (PPACK; Hematologic Technologies, Essex Junction, VT), Dade® Innovin® PT reagent (Siemens, Malvern, PA), Enzygnost® F1+2 monoclonal kit (Siemens Healthcare Diagnostic, Tarrytown, NY), D-dimer ELISA kit (Abcam, Cambridge, MA), Human Fibrinopeptide A (FPA) ELISA Kit (MyBioSource, San Diego, CA), ethylenediaminetetraacetic acid (EDTA, Sigma, St. Louis, MO), H-Gly-Pro-Arg-Pro-OH (GPRP, EMD Chemicals, San Diego, CA), Fluorescein Phe-Pro-Arg-chloromethylketone (fluorescein-PPACK; Hematologic Technologies, Essex Junction, VT), Sigmacote® siliconizing reagent (Sigma, St. Louis, MO), and Sylgard® 184 Silicone Elastomer kit (Dow Corning, Auburn, MI). O1A6 FXI antibody was a gift from Dr. Andras Gruber (Department of Biomedical Engineering, Oregon Health and Science University). See Supplement for catalog numbers.

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