Mouse anti myc

Manufactured by Abmart

Sourced in China

Mouse anti Myc is a laboratory reagent used for the detection and identification of the Myc protein in biological samples. It is a monoclonal antibody that binds specifically to the Myc protein, allowing for its visualization and quantification through various experimental techniques.

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Lab products found in correlation

Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Recombinant human ifn β, by Thermo Fisher Scientific (1 mentions) Mouse anti ha, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions) Puromycin, by Merck Group (1 mentions) Anti flag m2 affinity gel, by Merck Group (1 mentions) Rabbit anti gapdh, by Cell Signaling Technology (1 mentions) N ethylmaleimide nem, by Thermo Fisher Scientific (1 mentions) Mouse anti flag, by Cell Signaling Technology (1 mentions) Hrp goat anti mouse igg, by BioLegend (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Nuclear and cytoplasmic protein extraction kit, by Beyotime (1 mentions) Sirt1, by Cell Signaling Technology (1 mentions) Anti bdnf, by Abcam (1 mentions) Anti β tubulin, by Abcam (1 mentions) Rabbit anti sox4, by Abcam (1 mentions) Anti mapk1, by Abcam (1 mentions) Anti flag monoclonal antibody, by Abmart (1 mentions)

Close spelling variants of this product

Anti-Myc (27 citations)

4 protocols using mouse anti myc

Antibody Panel for Viral Protein Detection

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The following antibodies were purchased from Cell Signaling Technology (CST, Danvers, USA), rabbit anti GAPDH (Cat # 2118), mouse anti Flag (Cat # 8146), mouse anti HA (Cat # 2367) and anti-rabbit IgG HRP-linked Antibody (Cat # 7074). Others were obtained as follows; mouse anti Myc (Cat # M20002 Abmart, Shanghai, China), anti-Flag M2 Affinity Gel (Cat # A2220 Sigma-Aldrich, St Louis, USA), rabbit polyclonal anti DENV2 NS3 (Cat # PA5-32199 Thermo Fisher Scientific, Waltham, USA), rabbit polyclonal anti DENV2 NS4B (Cat # GTX113374 GeneTeX, Irvine, USA), rabbit polyclonal anti ZIKV NS3 (Cat # GTX133309 GeneTeX, Irvine, USA), rabbit anti TRIM69 (RNF36, Cat # ab111943 Abcam, Cambridge, UK), and HRP Goat anti-mouse IgG (Cat # 405306 Biolegend, San Diego, USA).
Lipofectamine 2000 was purchased from Life Technologies. MG132, puromycin, and dimethylsulfoxide (DMSO) were purchased from Sigma-Aldrich. N-ethylmaleimide (NEM) was obtained from Thermo Fisher Scientific. Protease inhibitor (PI) was from CST. Recombinant human IFN-β was from PeproTech (Rocky Hill, USA).

Wang K., Zou C., Wang X., Huang C., Feng T., Pan W., Wu Q., Wang P, & Dai J. (2018). Interferon-stimulated TRIM69 interrupts dengue virus replication by ubiquitinating viral nonstructural protein 3. PLoS Pathogens, 14(8), e1007287.

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Isolation and Analysis of Cellular Proteins

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Total protein from gastric cancer cells and tissues were isolated with RIPA lysis buffer (Beyotime), and cytosolic and nuclear protein fractions were purified with a nuclear and cytoplasmic protein extraction kit (Beyotime). Western blot analysis and immunoprecipitation were performed as previously described.⁵² (link) The intensity of the bands in western blot analysis was quantified using ImageJ (NIH). Antibodies against CD44, Sirt1, ATF2, Pak1, and PARP were purchased from Cell Signaling Technology, P-ATF2 ser62 antibody was from Abnova, and mouse anti-myc and anti-FLAG monoclonal antibody were from Abmart. Rabbit anti-SOX4, anti-BDNF, anti-HBEGF, anti-MAPK1, and anti-β tubulin were purchased from Abcam, and mouse anti-GAPDH was purchased from KangCheng Bio.

Liu F., Cheng Z., Li X., Li Y., Zhang H., Li J., Liu F., Xu H, & Li F. (2017). A Novel Pak1/ATF2/miR-132 Signaling Axis Is Involved in the Hematogenous Metastasis of Gastric Cancer Cells. Molecular Therapy. Nucleic Acids, 8, 370-382.

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Duck Hepatitis A Virus Infection Assay

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The DHAV-1 H strain (GenBank accession number: JQ301467.1), the engineered E. coli DH5α bacterium, and duck embryo fibroblasts (DEFs) used in this study were provided by the Sichuan Agricultural University Poultry Disease Prevention Research Center. A mouse anti-Flag monoclonal antibody (Cat: M185-3 S) and a mouse anti-HA monoclonal antibody (Cat: M132-3) were purchased from Medical & Biological Laboratories Co., Ltd. A rabbit anti-duck IRF7 polyclonal antibody, a HRP-conjugated goat anti-mouse IgG heavy chain antibody (Cat: AS064), and a HRP-conjugated goat anti-mouse IgG light chain antibody (Cat: AS062) were prepared by ABclonal Technology Co., Ltd. A rabbit anti-beta (β)-actin antibody (Cat: 20536-1-AP) was obtained from Proteintech Co., Ltd. A rabbit anti-HA monoclonal antibody (Cat: AF2305), a mouse IgG antibody (Cat: A7028), and a HRP-conjugated goat anti-mouse IgG (Cat: A0216) were purchased from Beyotime Co., Ltd. A rabbit anti-Histone H3 (Cat: TA6358), a mouse anti-Myc (Cat: T62076M), and a mouse anti-beta (β)-tubulin monoclonal antibody (Cat: T63017-2) were purchased from Abmart Co., Ltd. A rabbit anti-VP3 antibody was prepared in our laboratory [19 ]. An Alexa Fluor^™ 568 goat anti-mouse IgG antibody (Cat: A11004) and an Alexa Fluor^™ 488 goat anti-rabbit IgG antibody (Cat: A11008) were purchased from ThermoFisher Scientific Co., Ltd.

Xia X., Cheng A., Wang M., Ou X., Sun D., Zhang S., Mao S., Yang Q., Tian B., Wu Y., Huang J., Gao Q., Jia R., Chen S., Liu M., Zhao X.X., Zhu D., Yu Y, & Zhang L. (2023). DHAV 3CD targets IRF7 and RIG-I proteins to block the type I interferon upstream signaling pathway. Veterinary Research, 54, 5.

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Agrobacterium-Mediated Transient Expression

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The agrobacterium GV3101 cells harboring the pSuper-1300-35S-SAUR and vectors were inoculated in LB culture medium and cultured overnight at 28 °C. The agrobacterium culture was centrifuged and suspended with IM (inoculation medium: 10 mM MES (2-(N-morpholino)ethanesulfonic acid), 10 mM MgCl₂, 500 µM AS (acetosyringone)). After 2 h incubation, at room temperature in darkness, the agrobacterium culture was centrifuged and suspended again with IM, and the final concentration of agrobacterium (measured by OD₆₀₀) was adjusted to 0.3, 0.6, and 0.9. The agrobacterium inoculum was infiltrated into healthy and fully expanded cotyledons of 1-week-old wax gourd plants using a needleless syringe. Total protein was extracted at 3 dpi and mouse anti-MYC (ABmart, Lot number: 294166) antibody was used at 1:3000 concentration for immunoblots. The cell size of cotyledonary epidermal pavement cells was investigated, at 5 dpi, on free-hand sections. Cell size in each section was calculated by the ImageJ software.

Xie D., Xu Y., Wang J., Liu W., Zhou Q., Luo S., Huang W., He X., Li Q., Peng Q., Yang X., Yuan J., Yu J., Wang X., Lucas W.J., Huang S., Jiang B, & Zhang Z. (2019). The wax gourd genomes offer insights into the genetic diversity and ancestral cucurbit karyotype. Nature Communications, 10, 5158.

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