For high resolution image acquisitions of egg chambers, laser scanning confocal microscopy was carried out using a Leica TCS SP8 microscope with a HC PL APO 63x/1.30 Glycerol CORR CS2 glycerol-immersion objective. Images were acquired in the Leica Lightning mode with adaptive deconvolution. Low resolution imaging was performed with a HC PL APO 20X/0.75 CS2 Air objective. For in vitro droplet experiments, images were acquired with an HC PL APO 40x/1.10 W CORR CS2 water-immersion objective without subsequent deconvolution.
Lightning mode
Manufactured by Leica
The Lightning mode is an advanced feature in Leica's lab equipment. It enables rapid acquisition of images, capturing high-speed events with exceptional clarity and precision.
Automatically generated - may contain errors
Lab products found in correlation
Plan apochromat oil objective, by Leica (2 mentions)
Las x software, by Leica (2 mentions)
Lsm 700, by Zeiss (2 mentions)
Dmi6000, by Leica (2 mentions)
Plan apochromat oil objective, by Zeiss (2 mentions)
Tcs sp8 inverted microscope, by Leica (2 mentions)
Las x, by Leica (1 mentions)
Hc pl apo 63x 1.40 oil cs2, by Leica (1 mentions)
Hc pl apo cs2 63 1.40 oil, by Leica (1 mentions)
Las x v 3, by Leica (1 mentions)
3 protocols using lightning mode
1
High-Resolution Egg Chamber Imaging
2
Fluorescence Imaging of Redox State and Apoptosis
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Ratio-metric imaging of Grx1-rGFP was performed on an inverted microscope (DMI 6000; Leica) equipped with hybrid photon counting detectors (HyD; Leica). The sample was excited sequentially frame by frame with 408 nm and 488 nm with the detection set to 500-535 nm. Fluorescence was collected through a 63x 1.40 NA oil immersion objective (HC PL APO 63x/1.40 Oil CS2; Leica). Images were captured using the LAS X software (Leica). All imaging was performed at 37˚C in pre-warmed Leibovitz medium for maximum 90 min per sample.
For confocal microscopy of fixed BrU samples the imaging was performed using a Leica TCS SP8 inverted microscope equipped with 405-, 488-, 552-and 638-nm lasers and a Plan-Apochromat oil objective (×63, NA 1.4). The Lightning mode (Leica) to generate deconvolved images. Microscope acquisitions were controlled by LAS X (v. 3.5.2) software from Leica.
CLEM and Caspase 3/7 samples were imaged on a Zeiss LSM 700 inverted confocal microscope with equipped with a Plan-Apochromat oil objective (×63, NA 1.40) and 488-nm and 555-nm solid-state lasers and three photomultipliers. Acquisitions were controlled by the Zeiss Zen (v. 6.0.0) software.
For confocal microscopy of fixed BrU samples the imaging was performed using a Leica TCS SP8 inverted microscope equipped with 405-, 488-, 552-and 638-nm lasers and a Plan-Apochromat oil objective (×63, NA 1.4). The Lightning mode (Leica) to generate deconvolved images. Microscope acquisitions were controlled by LAS X (v. 3.5.2) software from Leica.
CLEM and Caspase 3/7 samples were imaged on a Zeiss LSM 700 inverted confocal microscope with equipped with a Plan-Apochromat oil objective (×63, NA 1.40) and 488-nm and 555-nm solid-state lasers and three photomultipliers. Acquisitions were controlled by the Zeiss Zen (v. 6.0.0) software.
3
Ratiometric Imaging of Mitochondrial Redox
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Ratiometric imaging of mito-GRX1-roGFP was performed on an inverted microscope (DMI 6000; Leica) equipped with hybrid photon counting detectors (HyD; Leica). The sample was excited sequentially frame by frame at 408 nm and 488 nm with the detection set to 500-535 nm. Fluorescence was collected through a 63× 1.40 NA oil immersion objective (HC PL APO 63×/1.40 Oil CS2; Leica). Images were captured using the LAS X software (Leica). All imaging was performed at 37 °C in pre-warmed Leibovitz medium for maximum 90 min per sample.
For confocal microscopy of fixed BrU samples the imaging was performed using a Leica TCS SP8 inverted microscope equipped with 405-, 488-, 552-and 638-nm lasers and a Plan-Apochromat oil objective (×63, NA 1.4). The Lightning mode (Leica) was used to generate deconvolved images. Microscope acquisitions were controlled by LAS X (v. 3.5.2) software from Leica.
CLEM and caspase 3/caspase 7 samples were imaged on a Zeiss LSM 700 inverted confocal microscope equipped with a Plan-Apochromat oil objective (×63, NA 1.40) and 488-nm and 555-nm solid-state lasers and three photomultipliers. Acquisitions were controlled by the Zeiss Zen (v. 6.0.0) software.
For confocal microscopy of fixed BrU samples the imaging was performed using a Leica TCS SP8 inverted microscope equipped with 405-, 488-, 552-and 638-nm lasers and a Plan-Apochromat oil objective (×63, NA 1.4). The Lightning mode (Leica) was used to generate deconvolved images. Microscope acquisitions were controlled by LAS X (v. 3.5.2) software from Leica.
CLEM and caspase 3/caspase 7 samples were imaged on a Zeiss LSM 700 inverted confocal microscope equipped with a Plan-Apochromat oil objective (×63, NA 1.40) and 488-nm and 555-nm solid-state lasers and three photomultipliers. Acquisitions were controlled by the Zeiss Zen (v. 6.0.0) software.
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