129s4 svjaej

Manufactured by Jackson ImmunoResearch

Sourced in United States

The 129S4/SvJaeJ is a mouse strain that can be used as a source of cells, tissues, or organs for research purposes. It is a subline of the 129S mouse strain and is commonly used in the study of gene function and development.

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5 protocols using 129s4 svjaej

Ush2a knockout mouse model for research

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Six Ush2a knockout (KO) male mice^{27 (link)} were provided by Dr. Jun Yang (University of Utah), and were re-derived on an 129S4/SvJaeJ background strain at the Gene Targeting and Transgenic Facility (GTTF) at UConn Health. All subjects were single housed in standard Plexiglass mouse-tubs (12 h/12 h light-dark cycle), with food and water available ad libitum. F1 subjects were delivered to the University of Connecticut where they were crossed with six wild-type (WT) controls (129S4/SvJaeJ; stock number 009104) purchased from The Jackson Laboratory (Bar Harbor, ME). The resulting F2 offspring were heterozygous (HT) for the Ush2a gene, which shows 71% identity with its Human orthologue. Breeding pairs (HT × HT) were used to generate the experimental subjects, such that all genotypes (homozygous knockout, heterozygous, and wild-type) were represented within-litter (F3). F3 genotypes were determined via PCR of earpunch DNA using the following DNA primers: Common (5′-GTGAATACAGGCACCTCTGAATGTGAC-3′), WT (5′-GTCACGGCTGAATCCCGAAGC-3′), KO (5′-GAGATCAGCAGCCTCTGTTCCAC-3′). Twelve WT male mice, 12 HT male mice, and 11 Ush2a KO male mice from F3 were randomly selected for behavioural testing as outlined below (12 WT, 11 HT and 11 KO mice were used when recording ultrasonic vocalizations).

Perrino P.A., Talbot L., Kirkland R., Hill A., Rendall A.R., Mountford H.S., Taylor J., Buscarello A.N., Lahiri N., Saggar A., Fitch R.H, & Newbury D.F. (2020). Multi-level evidence of an allelic hierarchy of USH2A variants in hearing, auditory processing and speech/language outcomes. Communications Biology, 3, 180.

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Genetic Background in Drug Abuse Sensitivity

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Male 129S1/SvlmJ, 129S4/SvJaeJ, 129S8/SvEvNimrJ, A/J, AKR/J, BALB/cJ, BTBRT < +>ltpr3 < tf>/J, C3H/HeJ, C57BL/6J, CBA/J, DBA/1J, DBA/2J, FVB/NJ, LP/J, MA/MyJ, NZB/BINJ, SJL/J, SM/J, & SWR/J mice were obtained from Jackson Laboratory (Bar Harbor, ME). 129S2/SvPasCrl were obtained from Charles River (Wilmington, MA). Individual strain characteristics can be found on https://mice.jax.org/ and https://www.criver.com/ (129S2). These mice were part of a larger project examining the influence of genetic background on sensitivity to drugs of abuse. All mice were 10–15 weeks of age for behavioral testing and tissue collection (n = 9–13 per strain). All mice were group-housed [with the exception of SJL/J, which were single-housed due to excessive social aggression characteristic of this strain; (43 (link))] with a 12-h light/dark cycle and unlimited access to food and water. All behavioral testing occurred between 8:00 A.M. and 5:00 P.M. All procedures were conducted in accordance with the NIH Guide for the Care and Use of Laboratory Animals and approved by the Penn State University IACUC Committee.

Mooney-Leber S.M., Zeid D., Garcia-Trevizo P., Seemiller L.R., Bogue M.A., Grubb S.C., Peltz G, & Gould T.J. (2021). Genetic Differences in Dorsal Hippocampus Acetylcholinesterase Activity Predict Contextual Fear Learning Across Inbred Mouse Strains. Frontiers in Psychiatry, 12, 737897.

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Mouse Strain Derivation for OG2-MEFs

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C57BL/6J, CBA/CaJ, and 129S4/SvJaeJ mice were purchased from the Jackson Laboratory. C57BL/6J, CBA/CaJ, and 129S4/SvJaeJ were used for generating OG2-MEFs. Animal experiments were performed according to the guidelines for the Care and Use of Laboratory Animals of the National Institutes of Health.

Ping W., Hu J., Hu G., Song Y., Xia Q., Yao M., Gong S., Jiang C, & Yao H. (2018). Genome-wide DNA methylation analysis reveals that mouse chemical iPSCs have closer epigenetic features to mESCs than OSKM-integrated iPSCs. Cell Death & Disease, 9(2), 187.

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Mouse Melanoma and Lung Cancer Models

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All mice were housed in the animal facility at Moffitt Cancer Center under specific pathogen-free conditions. C57BL/6 and SCID mice were obtained from Charles River (Wilmington, MA), 129S4/SvJaeJ were obtained from Jackson Laboratories (Bar Harbor, ME). IFNAR1^−/−, CD40^−/−, BATF3^−/− and CCR7^−/− mice were obtained from Jackson Laboratories and bred inhouse. All animal experiments were approved by the University of South Florida Institutional Animal Care and Use Committee.
B16-F10 mouse melanoma cell line was from ATCC and was cultured in DMEM (Cat No. 15-017-cv, CORNING) with 10% FBS (Cat No. A52568-01, GIBCO). Zs-Green overexpressing B16-F10 cells were kindly provided by Dr. Max Krummel (University of California, San Francisco). Mouse 344SQ cell line (kindly provided by Dr. J. Kurie, MD Anderson Cancer Center) with KRAS G12D and TP53 mutations (R172H) was maintained in RPMI 1640 (Cat No. 10-040-cv, CORNING) with 10% FBS. Human A549 lung cancer cell line was obtained from the Moffitt Lung Cancer Center of Excellent repository, authenticated by short tandem repeat analysis, and maintained in RPMI 1640 with 10% FBS. For tumor growth studies, cell lines were harvested in logarithmic growth phase after being cultured for less than 2 weeks. Cell lines tested negative for mycoplasma contamination (PlasmoTest, Mycoplasma Detection Kit from InvivoGen, San Diego, CA).

Cannone J.J., Subramanian S., Schnare M.N., Collett J.R., D'Souza L.M., Du Y., Feng B., Lin N., Madabusi L.V., Müller K.M., Pande N., Shang Z., Yu N, & Gutell R.R. (2002). The Comparative RNA Web (CRW) Site: an online database of comparative sequence and structure information for ribosomal, intron, and other RNAs. BMC Bioinformatics, 3, 2.

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Inbred Mouse Strain Liver Dissection

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The subjects were adult (10–13 weeks at time of liver dissection), male mice of eight inbred mouse strains: 129S2/SvPasCrl, 129S4/SvJaeJ, 129S8/SvEvNimrJ, BTBR T+ Itpr3tf/J, C57BL/6J, MA/MyJ, NZB/BINJ and SM/J (n = 9 per treatment group per strain, all strains aside from 129S2/SvPasCrl purchased from Jackson Laboratory, Bar Harbor, ME, USA; 129S2/SvPasCrl purchased from Charles River, Wilmington, MA, USA). All mice were group-housed in the same colony room with a 12 h light/dark cycle and ad libitum access to food and water. All procedures were performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals and were approved by the Pennsylvania State University IACUC committee.

Zeid D., Mooney-Leber S., Seemiller L.R., Goldberg L.R, & Gould T.J. (2021). Terc Gene Cluster Variants Predict Liver Telomere Length in Mice. Cells, 10(10), 2623.

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