MiR-375 mimic and miR-control were purchased from GenePharma (Shanghai, China). MiRNA transfections were performed using Lipofectamine 2000 (Invitrogen, Carlsbad, USA), according to the manufacturer’s instructions. The extent of overexpression was evaluated by qRT-PCR 24 h after transfection. MiR-375 mimic and miR-control lentiviruses were synthesised by Genechem (Shanghai, China). For stable overexpression of miR-375 in glioma cells, MiR-375 mimic or miR-control lentiviruses were added to cells, according to the manufacturer’s protocol. After 24 h, clones with stable expression were selected by incubating cells for 3 weeks with 5 μg/mL puromycin (Sigma, St Louis, USA) in complete medium containing 10% FBS. The sequences of miR-control and MiR-375 mimic were 5′-TTCTCCGAACGTGTCACGT-3′ and 5′-TTTGTTCGTTCGGCTCGCGTGA-3′, respectively.
Mir 375 mimic
Manufactured by Genechem
Sourced in China
The MiR-375 mimic is a synthetic double-stranded RNA molecule designed to mimic the endogenous microRNA miR-375. MicroRNAs are small, non-coding RNA molecules that play a crucial role in the regulation of gene expression. The MiR-375 mimic is a laboratory tool used in research to study the biological functions and target genes of miR-375.
Automatically generated - may contain errors
2 protocols using mir 375 mimic
1
Overexpression of miR-375 in Glioma Cells
2
Overexpression of miR-375 in Glioma Cells
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MiR-375 mimic and miR-control were purchased from GenePharma (Shanghai, China). MiRNA transfections were performed using Lipofectamine 2000 (Invitrogen, Carlsbad, USA), according to the manufacturer's instructions. The extent of overexpression was evaluated by qRT-PCR 24 h after transfection. MiR-375 mimic and miR-control lentiviruses were synthesised by Genechem (Shanghai, China). For stable overexpression of miR-375 in glioma cells, MiR-375 mimic or miR-control lentiviruses were added to cells, according to the manufacturer's protocol. After 24 h, clones with stable expression were selected by incubating cells for 3 weeks with 5 μg/mL puromycin (Sigma, St Louis, USA) in complete medium containing 10 % FBS. The sequences of miR-control and MiR-375 mimic were 5 -TTCTCCGAACGTGTCACGT-3 and 5 -TTTGTTCGTTCGGCTCGCGTGA-3 , respectively.
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