The right frontal cortical tissues were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer (PH 7.4). The samples were then rinsed three times in 0.1 M phosphate buffer (PH 7.4) and further fixed in 1% osmic acid (Ted Pella) for 2 h. After another round of rinsing in 0.1 M phosphate buffer (PH 7.4), the samples were dehydrated in various concentrations of alcohol for 20 min and 100% acetone for 15 min twice. Following dehydration, the samples were embedded in resin and left to polymerize at 60°C for at least 48 h. Ultrathin sections (60–80 nm thick) were cut with a diamond knife (Daitome) on an ultra‐thin microtome (Leica). These sections were placed on 150 mesh copper grids and stained with 2% uranyl acetate saturated alcohol solution to avoid light staining for 8 min. Finally, the sections were stained with 2.6% lead citrate to avoid CO2 for 8 min and rinsed in ultrapure water three times. The high‐resolution transmission electron microscopy (TEM) images were acquired on a TEM instrument (Hitachi).
Tem instrument
Manufactured by Hitachi
Sourced in Japan
The TEM instrument from Hitachi is a transmission electron microscope that uses a beam of electrons to create highly detailed images of small-scale structures and materials. The core function of this instrument is to provide high-resolution, magnified views of samples by transmitting a beam of electrons through a thin specimen.
Automatically generated - may contain errors
5 protocols using tem instrument
1
Ultrastructural Analysis of Frontal Cortex
2
Physicochemical Characterization of CS-NPs
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A Zetasizer Nano ZS (Malvern Instruments, Malvern, UK) was employed to assess the size and zeta potential of the prepared CS-NPs (antagomir-182-5p). The assessments were carried out three times at pH 7.4 at 25℃. Transmission Electron Microscopy (TEM) was utilized to perform the morphological examination of CS-NPs (antagomir-182-5p). Next, CS-NPs solution was dropped onto a carbon-coated copper grid and dried at RT. A TEM instrument (Hitachi, Tokyo, Japan) was then applied to evaluate the samples.
3
Characterization of Polyplex Nanoparticles
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Example 8
Polyplex nanoparticles were prepared by mixing SP, LP1, LP2, and PEI with plasmid pGFP at varying N/P ratios. The size and charge properties of polyplex nanoparticles were characterized by Dynamic Light Scattering (DLS) spectroscopy and zeta potential measurements, which were achieved on a Malvern Nanosizer ZS (Malvern, USA) equipped with a 633 nm laser. As illustrated in
Example 9
Polyplex nanoparticles prepared at N/P ratios of 5 from Example 8 were diluted with DI-water to a final pGFP concentration of 40 μg/mL, applied for 10 min in 6 μL onto the carbon surface of 400 mesh copper electron microscope grids (Teda Pella, covered with Formvar and carbon films), washed with water, stained by uranyl acetate (0.8% in methanol, freshly filtered on 20 nm Whatman membrane), and examined on a Hitachi TEM instrument at a magnification of 10,000ט100,000×. The images were saved in .TIFF format and treated with Image J software (version 1.46r). As illustrated in
4
Characterization of Hemoglobin Nanoparticles
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The particle size distribution and Zeta-potential of H-NPs were measured by the dynamic light scattering instrument (Malvern, UK). H-NPs were observed by Transmission Electron Microscopy (TEM) instrument (HITACHI) and pre-negative staining by 2% phosphotungstic acid (PTA) was performed. The uv-vis spectra of oxy/deoxyhemoglobin- and oxygen- loaded/unloaded H-NPs were measured by ultraviolet-visible spectrophotometer (Persee).
5
Visualizing PCV4 VLP Assembly by TEM
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The assembly of PCV4 VLPs before puri cation and at each puri cation step was visualized using transmission electron microscopy (TEM). Samples were placed on copper mesh and air-dried at room temperature. Negative staining was performed by incubating the samples in 2.5% phosphotungstic acid for 1 minute. After drying, the samples were observed using a TEM instrument (HITACHI, Tokyo, Japan) for visualization.
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