Cd8α percp cyanine 5

Manufactured by BioLegend

CD8α-PerCP/Cyanine 5.5 is a fluorescently-labeled antibody that binds to the CD8α cell surface marker. It is designed for use in flow cytometry applications to identify and characterize CD8+ T cells.

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Lab products found in correlation

Cd69 fitc, by BioLegend (2 mentions) Cd4 bv785, by BioLegend (2 mentions) Cd44 pe cyanine 7, by BioLegend (2 mentions) Cd103 pe, by BioLegend (2 mentions) Fcγr antibody, by BioLegend (1 mentions) Fixable viability dye efluor 506, by BD (1 mentions)

2 protocols using cd8α percp cyanine 5

T Cell Phenotyping for SARS-CoV-2 Immunity

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For T cell analysis, single cell suspensions were incubated with FcγR antibody (clone 93, BioLegend) to block non-specific antibody binding, followed by staining with a cocktail of labeled mAbs including Fixable Viability dye eFluor506, CD3e-BV711 (1:100, clone:145–2C11, BD Biosciences), CD8α-PerCP/Cyanine 5.5 (1:100, 53–6.7, BioLegend), CD4-BV785 (1:100, clone: RM4–5, BioLegend), CD44-PE/Cyanine 7 (1:100, clone: IM7, BioLegend), CD69-FITC (1:100, clone: H1.2F3, BioLegend), CD103-PE (1:100, clone: 2E7, BioLegend) and APC-labeled SARS-CoV-2 S- specific tetramer (MHC class I tetramer, residues 539–546, VNFNFNGL, H-2K(B) for 60 min at room temperature. Cells were washed twice with FACS buffer, fixed with 2% paraformaldehyde (PFA) for 20 min prior to data acquisition. Data were acquired on an Aurora (Cytek) spectral flow cytometer and analyzed in FlowJo v10 software.

Ying B., Darling T.L., Desai P., Liang C.Y., Dmitriev I.P., Soudani N., Bricker T., Kashentseva E.A., Harastani H., Schmidt A.G., Curiel D.T., Boon A.C, & Diamond M.S. (2023). A bivalent ChAd nasal vaccine protects against SARS-CoV-2 BQ.1.1 and XBB.1.5 infection and disease in mice and hamsters. bioRxiv.

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Multiparametric T Cell Profiling

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For T cell analysis, single-cell suspensions were incubated with FcγR antibody (clone 93, BioLegend) to block non-specific antibody binding, followed by staining with a cocktail of labeled monoclonal antibodies, including Fixable Viability dye eFluor506, CD3e-BV711 (1:100, clone 145-2C11, BD Biosciences), CD8α-PerCP/Cyanine 5.5 (1:100, 53-6.7, BioLegend), CD4-BV785 (1:100, clone RM4-5, BioLegend), CD44-PE/Cyanine 7 (1:100, clone IM7, BioLegend), CD69-FITC (1:100, clone H1.2F3, BioLegend), CD103-PE (1:100, clone 2E7, BioLegend) and APC-labeled SARS-CoV-2 S-specific tetramer (MHC class I tetramer, residues 539–546, VNFNFNGL, H-2K^b) for 60 min at room temperature. Cells were washed twice with FACS buffer and fixed with 2% paraformaldehyde for 20 min before data acquisition. Data were acquired on an Aurora (Cytek) spectral flow cytometer and analyzed with FlowJo v10 software.

Ying B., Darling T.L., Desai P., Liang C.Y., Dmitriev I.P., Soudani N., Bricker T., Kashentseva E.A., Harastani H., Raju S., Liu M., Schmidt A.G., Curiel D.T., Boon A.C, & Diamond M.S. (2024). Mucosal vaccine-induced cross-reactive CD8+ T cells protect against SARS-CoV-2 XBB.1.5 respiratory tract infection. Nature Immunology, 25(3), 537-551.

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