The concentrations of agomelatine were quantified using a Waters ACQUITY UPLC system (Waters Corp., Milford, MA, USA) coupled to a XEVO TQD triple quadrupole tandem mass spectrometer (Waters Corp.) with an electrospray ionization (ESI+) interface. Chromatographic separations were performed on an ACQUITY BEH C18 column (2.1×50 mm, 1.7 µm) with a C18 inline 0.2 mm stainless steel frit filter (Waters Corp.). The mobile phases consisted of water containing 0.1% FA and acetonitrile with isocratic elution in the ratio of 45:55. The flow rate was 0.3 mL/min with the injection volume of 2 µL. Mass spectrometric detection for quantitation by multiple reaction monitoring was performed on a XEVO TQD triple quadrupole mass spectrometer in the positive mode. The transitions were m/z 244.1 → 185.2, m/z 230.1 → 171.0, and m/z 237.1 → 194.2 for agomelatine, 7-desmethyl-agomelatine, and carbamazepine, respectively.
Xevo tqd triple quadrupole tandem mass spectrometer
Manufactured by Waters Corporation
Sourced in United States
The XEVO TQD is a triple quadrupole tandem mass spectrometer manufactured by Waters Corporation. It is a high-performance analytical instrument used for the detection and quantification of a wide range of compounds in complex matrices. The XEVO TQD employs the use of two quadrupole mass analyzers in tandem, allowing for precise and selective analysis of target analytes.
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2 protocols using xevo tqd triple quadrupole tandem mass spectrometer
1
UPLC-MS/MS Quantification of Agomelatine
2
Polar Compound Analysis in Cell Extracts
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The samples were analyzed following the protocol published by Virgiliou and co-workers [37 (link)], which allowed su to identify 100 different polar compounds. The Acquity H-Class UPLC system (Waters, Milford, MA, USA) was used to analyze polar compounds in cell extracts. The chromatograph was equipped with a YMC-Triart C18 (100 × 2.0 mm, 1.9 µm) column (YMC, Kyoto, Japan). Mass spectrometry (MS) data were obtained in negative mode in the 50 m/z to 250 m/z range with a Xevo TQD triple quadrupole tandem mass spectrometer (Waters, Milford, MA, USA) coupled with an electrospray ionization (ESI) ion source. The column was set at 40 °C. Elution was applied with a mobile phase of 0.1% formic acid water solution (solvent A) and acetonitrile (solvent B). Flow rate was set to 0.4 mL/min. The starting conditions were set to 95% of solvent A. Gradient elution was applied with the following proportions of solvent A: 0 to 0.2 min was set to 95%, 0.2 to 1.5 min was set to 10%, 1.5 min to 1.8 min was maintained at 90%, and 1.8 to 2 min was set back to the starting conditions. The settings for the mass spectrometer were as follows: capillary voltage was set to negative 2 kV, source temperature to 150 °C, desolvation gas (nitrogen) temperature to 400 °C, gas flow to 700 L/h and cone gas flow to −20 L/h. Cone voltage was set to 25 V.
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