Purelab purification system

Manufactured by Elga LabWater

Sourced in Canada

The PureLab purification system is a water purification solution designed to deliver high-quality purified water for laboratory applications. Its core function is to remove impurities and produce water with specified purity levels, meeting the requirements of various laboratory processes.

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Close spelling variants of this product

Purelab Ultra water purification system (4 citations) PURELAB flex 2 water purification system (3 citations) PURELAB® water purification system (3 citations)

5 protocols using purelab purification system

Lipid Extraction and Standards

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The following chemicals were purchased from Fisher Scientific (Ottawa, ON, Canada): LC-grade chloroform, methanol, acetonitrile, formic acid, acetic acid and ammonium acetate. For solutions preparation, deionized water (PURELAB Purification System, ELGA Labwater, ON, Canada) was employed. Phospholipids, sphingolipids and glycolipids standards were used (Avanti Polar Lipids Inc., Alabaster, AL, USA).

Vidal N.P., Pham H.T., Manful C., Pumphrey R., Nadeem M., Cheema M., Galagedara L., Leke-Aladekoba A., Abbey L, & Thomas R. (2018). The use of natural media amendments to produce kale enhanced with functional lipids in controlled environment production system. Scientific Reports, 8, 14771.

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Quantification of Isotopically Labeled Thyroid Hormone

Cited 1 time

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Acetonitrile (Ultra Chromasolv, LCMS grade), triethylammonium bicarbonate (TEAB) buffer (1 M), ethylenediamine-tetraacetic acid (EDTA, anhydrous), HSA (lyophilized powder, 97–99%), and porcine trypsin were from Sigma-Aldrich (St. Louis, MO). Methanol (Optima, LCMS grade), formic acid (Optima, LCMS grade), and iodoacetamide (IAA) were from Fisher Scientific (Pittsburgh, PA). Purified human Hb was purchased from MP Biomedicals, LLC (Santa Ana, CA). Isotopically labeled T3 (iT3) with sequence AL-[¹⁵N,¹³C-Val]-LIAFAQYLQQCPFEDH-[¹⁵N,¹³C-Val]-K was custom-made (>95%, BioMer Technology, Pleasanton, CA), and the carbamidomethylated iT3 (IAA-iT3)[18 (link)] was used as an internal standard to monitor retention time and mass drifts. Water was prepared with a PureLab purification system (18.2 mΩ cm resistivity at 25 °C; Elga LabWater, Woodridge, IL).

Yano Y., Grigoryan H., Schiffman C., Edmands W., Petrick L., Hall K., Whitehead T., Metayer C., Dudoit S, & Rappaport S. (2019). Untargeted adductomics of Cys34 modifications to human serum albumin in newborn dried blood spots. Analytical and bioanalytical chemistry, 411(11), 2351-2362.

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Comprehensive Lipid Standard Preparation

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HPLC grade acetonitrile, chloroform and methanol were purchased from Fisher Scientific, Canada; deionized water was obtained from PURELAB Purification System (ELGA Labwater, ON, Canada). HPLC grade acetic acid, formic acid, ammonium formate and ammonium acetate were purchased from Sigma-Aldrich (ON, Canada). Each lipid class was purchased as individual from Avanti Polar Lipids (Alabaster, AL, USA) and used to make a complex lipid standard mixture consisting SQDG 16:0/16:0, MGDG 16:3(7Z,10Z,13Z)/18:3(9Z,12Z,15Z), PA 18:1(9Z)/18:1(9Z), PG 18:0/20:4(5Z,8Z,11Z,14Z), PC 18:0/20:4(5Z,8Z,11Z,14Z), PE 18:0/20:4(5Z,8Z,11Z,14Z), diether PC O-18:0/O-18:0, plasmalogen PC P-18:0/20:4(5Z,8Z,11Z,14Z), plasmalogen PE P-18:0/20:4(5Z,8Z,11Z,14Z), MMPE 16:0/16:0 and DMPE 16:0/16:0, LPA 20:4(5Z,8Z,11Z,14Z), LPC 18:1(9Z), LPE 18:0, plasmalogen LPE P-18:0; PI 18:0/20:4(5Z,8Z,11Z,14Z); DLCL 18:2(9Z,12Z)/18:2(9Z,12Z), and SM d18:1/18:0. Aliquots of the prepared standard mixture were used for method development.

Pham T.H., Zaeem M., Fillier T.A., Nadeem M., Vidal N.P., Manful C., Cheema S., Cheema M, & Thomas R.H. (2019). Targeting Modified Lipids during Routine Lipidomics Analysis using HILIC and C30 Reverse Phase Liquid Chromatography coupled to Mass Spectrometry. Scientific Reports, 9, 5048.

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Hemoglobin Quantification Using Labeled T3

Cited 1 time

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Acetonitrile (Ultra Chromasolv, LCMS grade), triethylammonium bicarbonate (TEAB) buffer (1 M), ethylenediamine-tetraacetic acid (EDTA, anhydrous), and porcine trypsin were from Sigma-Aldrich (St. Louis, MO). Methanol (Optima, LCMS grade), formic acid (Optima, LCMS grade), and iodoacetamide (IAA) were from Fisher Scientific (Pittsburgh, PA). Purified human hemoglobin was from MP Biomedicals, LLC (Santa Ana, CA). Isotopically labeled T3 (iT3) with sequence AL-[¹⁵N,¹³C-Val]-LIAFAQYLQQCPFEDH-[¹⁵N,¹³C-Val]-K was custom-made (>95%, BioMer Technology, Pleasanton, CA), and the carbamidomethylated iT3 (IAA-iT3)[14 (link)] was used as an internal standard to monitor retention time and mass drifts, as well as drift in instrument performance. Water was prepared with a PureLab purification system (18.2 mΩ cm resistivity at 25 °C; Elga LabWater, Woodridge, IL).

Yano Y., Schiffman C., Grigoryan H., Hayes J., Edmands W., Petrick L., Whitehead T., Metayer C., Dudoit S, & Rappaport S. (2019). Untargeted Adductomics of Newborn Dried Blood Spots Identifies Modifications to Human Serum Albumin Associated with Childhood Leukemia. Leukemia research, 88, 106268.

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Proteomics Workflow for T3 Peptide Quantification

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Triethylammonium bicarbonate buffer (TEAB), ethylenediamine-tetraacetic acid (EDTA, anhydrous), ammonium sulfate, porcine trypsin, HSA (lyophilized powder, 97–99%), acetonitrile (Ultra CHROMASOLV®, LC-MS grade), acetic acid (LCMS grade), L-glutathione (reduced), L-cysteine, L-homocysteine, iodine, iodoacetamide (IAA), ammonium sulfate, dimethyl sulfoxide (DMSO), hydrogen peroxide (30% aqueous solution) were from Sigma-Aldrich (St. Louis, MO). Methanol (Optima®, LCMS grade), tris(2-carboxyethyl)phosphine (TCEP), formic acid (Optima®, LCMS Grade), were from Fisher Scientific (Pittsburgh, PA). Purified water (18.2 mΩ cm resistivity at 25°C) was prepared with a PureLab purification system (Elga LabWater, Woodridge, IL). The following chemicals were custom synthesized: T3 peptide with sequence ALVLIAFAQYLQQCPFEDHVK (>97%, Biomatik, Wilmington, DE), isotopically modified T3 with sequence AL-[15N, 13C-Val]-LIAFAQYLQQCPFEDH-[15N, 13C-Val]-K (>95%, BioMer Technology, Pleasanton, CA).

Grigoryan H., Edmands W., Lu S.S., Yano Y., Regazzoni L., Iavarone A.T., Williams E.R, & Rappaport S.M. (2016). An adductomics pipeline for untargeted analysis of modifications to Cys34 of human serum albumin. Analytical chemistry, 88(21), 10504-10512.

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