R26 lacz

Mist1-CreERT2 mice (Shi et al., 2009 (link)), Cxcl12-dsRED mice (Ding and Morrison, 2013 (link)), Troy^−/− and Troy-BAC-CreERT2 mice (Fafilek et al., 2013 (link)), H/K-ATPase-Cxcl12 mice (Shibata et al., 2013 (link)), Eef1a1-LSL-Notch1(IC) mice (Buonamici et al., 2009 (link)), Wnt5a^flox mice (Miyoshi et al., 2012 (link)) were described previously. Cxcr4-EGFP mice were kindly provided by Richard J. Miller (Northwestern University Medical School, USA). LSL-Kras^G12D and LSL-Trp53^R172H mice were provided by Dr. Kenneth Olive (Columbia University, USA). Apc^flox mice were obtained from the National Cancer Institute (NCI). Lgr5-DTR-GFP mice were provided by Genentech. Cdh1^flox, R26-mTmG, R26-LacZ, R26-TdTomato, R26-Confetti, R26-EYFP, Cxcl12^flox, Tie2-Cre, Id2-GFP, and Cag-CreERT2 mice were purchased from the Jackson Laboratory. Cre recombinase was activated by oral administration of TAM (1–5mg/0.2mL corn oil, as indicated). All animal studies and procedures were approved by the ethics committees at Columbia University and the Academy of Sciences of the Czech Republic. Human stomach tissue sections were obtained from DGC patients who underwent surgical resection or endoscopic submucosal dissection from 2001 to 2012 at Gifu University Hospital, Gifu, Japan. All study protocols were approved by the ethics committees, and written informed consent was obtained from all patients.

All mouse strains used have been previously reported: aP2-Cre (Tang et al., 2008 (link)), Adipoq-Cre (#10803, Jackson Laboratories) (Eguchi et al., 2011 (link)), Ucp1-Cre (#24670, Jackson Laboratories) (Kong et al., 2014 (link)), Fabp4-Cre (#5069, Jackson Laboratories) (He et al., 2003 (link)), Flk1::Cre (Kdr::Cre) (#018976, Jackson Laboratories) (Motoike et al., 2003 (link)), R26-mTmG (#7676, Jackson Laboratories) (Muzumdar et al., 2007 (link)), R26-tdTomato (#7914, Jackson Laboratories) (Madisen et al., 2010 (link)), R26-LacZ (#3474, Jackson Laboratories) (Soriano, 1999 (link)), SmoM2 (#5130, Jackson Laboratories) (Mao et al., 2006 (link)), LSL-Kras^G12D (#8179, Jackson Laboratories) (Jackson et al., 2001 (link)) and Cdkn2A^Flox (Aguirre et al., 2003 (link)). For Kaplan-Meier tumor free survival analysis, Adipoq-Cre;Smo^M2/+ and Ucp1-Cre;Smo^M2/+ animals were observed from birth and confirmed to be tumor free at necropsy. For Kaplan-Meier survival analysis, aP2-Cre; LSL-Kras^G12D and aP2-Cre; LSL-Kras^G12D;Cdkn2a^Flox/Flox animals were observed from birth and sacrificed when showing signs of obvious tumor burden or other distress. Full necropsies were performed. All experimental procedures involving animals were reviewed and approved by SJCRH Institutional Animal Care and Use Committee.

All mouse strains were maintained on a C57/Bl6 background. Mist1^CreERT2 mice were produced as described (Shi et al., 2009 (link)) and crossed with the reporter strains R26^LacZ (Gt(ROSA)26Sor^tm1Sor), R26^Brainbow2.1 (Gt(ROSA)26Sor^{tm1(CAG-Brainbow2.1)Cle}/J), or R26^TdTomato (Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/J) (all from Jackson Labs). Standard genotyping was performed. All procedures and protocols were approved by the University Committee on Animal Resources at the University of Rochester.