Anti cd83

Manufactured by Beckman Coulter

Anti-CD83 is a monoclonal antibody that binds to the CD83 antigen, which is a marker expressed on the surface of mature dendritic cells. It is used in various research applications to identify and study dendritic cells.

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Lab products found in correlation

Anti cd86, by BD (3 mentions) Anti cd56, by Beckman Coulter (2 mentions) Anti hla dr, by BD (2 mentions) Fc500, by Beckman Coulter (2 mentions) Lsrfortessa, by BD (2 mentions) Anti ccr7, by R&D Systems (2 mentions) Anti cd3, by Beckman Coulter (1 mentions) Anti cd127, by BD (1 mentions) Anti cd117, by BD (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Anti cd80, by BD (1 mentions) Saponin, by Merck Group (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Cellquest software, by BD (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Anti ifn γ, by Thermo Fisher Scientific (1 mentions) Anti tnf α, by Thermo Fisher Scientific (1 mentions) Anti cd19, by BD (1 mentions) Anti il 2, by BD (1 mentions)

Spelling variants of this product

Anti-CD83-PE (3 citations)

3 protocols using anti cd83

Multiparametric Flow Cytometry Analysis

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Cells were incubated with fluorochrome-conjugated mAbs and analyzed on either an FC 500 (Beckman Coulter; Miami, FL) or an LSRFortessa (Becton Dickinson) flow cytometer with quadrants set to score > 99% of fluorochrome-conjugated mouse immunoglobulin (Ig) isotype controls (BD Pharmingen and DakoCytomation; Carpinteria, CA) as negative. FITC-, PE-, ECD-, APC-, PE-Cy5-, PE-Cy7-, PerCP-Cy5.5-, and AF700-conjugated mouse anti-human mAbs included anti-CD16 (clone 3G8), anti-NKG2D (clone 1D11), anti-CD117, anti-CD127, anti-CD14, anti-HLA-DR, and anti-CD86 (BD Pharmingen); anti-CD3, anti-CD56, anti-NKp46, and anti-CD83 (Beckman Coulter); and anti-NKB1 (clone DX9) (BioLegend; San Diego, CA). MAbs for sorting were specified above. DAPI (Invitrogen) was used to exclude dead cells. Flow cytometric data were analyzed with FlowJo 9.5 software (TreeStar; Ashland, OR).

Curran S.A., Romano E., Kennedy M.G., Hsu K.C, & Young J.W. (2014). Phenotypic and Functional Activation of Hyporesponsive KIRnegNKG2Aneg Human NK-Cell Precursors Requires IL-12p70 Provided by Poly(I:C)-Matured Monocyte-Derived Dendritic Cells. Cancer immunology research, 2(10), 1000-1010.

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Flow Cytometry Analysis of Immune Markers

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Flow cytometry was done using the following monoclonal antibodies (mAb) and appropriate isotype controls: anti-HLA class I (W6/32), anti-HLA DR/DP (Q5/13), anti-HLA DR, anti-CD70, anti-CD80 (all BD Biosciences), anti-CD14, anti-CD83 (both Beckman Coulter), anti-CD86 (BD PharMingen), and anti-CCR7 (R&D systems). For intracellular staining of the TAA NKI/beteb (IgG2b; purified antibody) against gp100, and T311 (IgG2a; Cell Marque Corp., Rocklin, CA) against tyrosinase were used. Cells were also stained intracellular with anti-CD40L (Beckman Coulter). For intracellular staining, cells were fixed for 4′ on ice in 4% (w/v) paraformaldehyde (Merck) in PBS, permeabilized in PBS/2%BSA/0.02% azide/0.5% saponin (Sigma-Aldrich) (PBA/saponin), and stained with mAb diluted in PBA/saponin/2%HS, followed by staining with Alexa488-labeled goat-anti-mouse (BD PharMingen). Flow cytometry was performed with FACSCalibur™ flow cytometer equipped with CellQuest software (BD Biosciences).

Bol K.F., Figdor C.G., Aarntzen E.H., Welzen M.E., van Rossum M.M., Blokx W.A., van de Rakt M.W., Scharenborg N.M., de Boer A.J., Pots J.M., olde Nordkamp M.A., van Oorschot T.G., Mus R.D., Croockewit S.A., Jacobs J.F., Schuler G., Neyns B., Austyn J.M., Punt C.J., Schreibelt G, & de Vries I.J. (2015). Intranodal vaccination with mRNA-optimized dendritic cells in metastatic melanoma patients. Oncoimmunology, 4(8), e1019197.

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Comprehensive Multiparametric Flow Cytometry Analysis

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PBMCs were incubated with fluorochrome-conjugated mAbs and analyzed on either an FC 500 (Beckman Coulter) or an LSRFortessa (Becton Dickinson) flow cytometer. FITC-, PE-, PE-Texas Red-, ECD-, APC-, PE-Cy5–, PE-Cy7–, PerCP-Cy5.5–, Pacific Blue-, and AF700-conjugated mouse anti-human mAbs included anti-CD3, anti-CD4, anti-CD8, anti-CD11c, anti-CD14, anti-CD16 (clone 3G8), anti-CD19, anti-CD25, ant-CD28, anti-CD45RA, anti-CD45RO, anti-CD80, anti-CD86, anti-CD123, anti-CTLA-4, anti–HLA-DR, anti-IL2, anti-Ki-67 (BD Pharmingen), anti-CD56, anti-CD83 (Beckman Coulter), anti-CD127, anti- IFNγ, anti-LAG-3, anti-PD-1, anti- TNFα (eBioscience), anti-CCR7, anti-TIM-3 (R&D Systems), and anti-CD57 (BioLegend). Nonreactive isotype-matched antibodies (Becton Dickinson, eBioscience, R&D Systems) were used as controls. LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies) facilitated exclusion of dead cells. Gates were set for collection and analysis of at least 20,000 live events. Data were analyzed with FlowJo 9.5 software (TreeStar).

Chung D.J., Pronschinske K.B., Shyer J.A., Sharma S., Leung S., Curran S.A., Lesokhin A.M., Devlin S.M., Giralt S.A, & Young J.W. (2015). T cell exhaustion in multiple myeloma relapse after autotransplant: Optimal timing of immunotherapy. Cancer immunology research, 4(1), 61-71.

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