For immunofluorescence (IF) staining of FGFR3, 5μm FFPE sections were subjected to antigen retrieval with citrate buffer for 8 min. Primary antibodies were: FGFR3-N (1:400, sc-13121, Santa Cruz Biotechnology), FGFR3-C (1:2000, sc-123, Santa Cruz Biotechnology), TACC3-N (1:600, ab134153, Abcam), and TACC3-C (1:300, NBP1-01032, Novus Biological). Secondary biotinylated antibodies were used at 1:50,000 followed by streptavidin and TSA Cy3-conjugated. Nuclei were counterstained with DAPI. For immunohistochemical analysis (IHC) of FGFR3 expression, antigen retrieval was performed for 12 min and FGFR-3 antibody (sc-13121, Santa Cruz Biotechnology) was diluted 1:500. Biotinylated anti-mouse antibody (1:30,000) and streptavidin were added before incubation with the chromogen. Nuclei were counterstaining with hematoxylin.
Ab134153
Manufactured by Abcam
Sourced in United States
Ab134153 is a laboratory equipment product offered by Abcam. It is a device designed to perform a specific function in the laboratory setting. The core function of this product is to [CORE FUNCTION]. No further details or interpretations about the intended use of this product are provided.
Automatically generated - may contain errors
Lab products found in correlation
Sc 13121, by Santa Cruz Biotechnology (1 mentions)
Sc 123, by Santa Cruz Biotechnology (1 mentions)
Ab24170, by Abcam (1 mentions)
Ab124767, by Abcam (1 mentions)
Ab8226, by Abcam (1 mentions)
Quick starttm bradford 1x dye reagent, by Bio-Rad (1 mentions)
Anti sqstm1 p62, by Cell Signaling Technology (1 mentions)
Chemidoc xrs image detector, by Bio-Rad (1 mentions)
Pa1 655, by Thermo Fisher Scientific (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Sds page gel, by Bio-Rad (1 mentions)
2 protocols using ab134153
1
Immunostaining Protocols for FGFR3 and TACC3
2
Cardiomyocyte Protein Analysis by Western Blot
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Cardiomyocytes were harvested in RIPA buffer with protease inhibitor tablets and sonicated on ice. The lysate was then centrifuged at 14,000 rpm at 4°C for 15 min, and the supernatant was reserved. Protein concentrations were determined using Quick StartTM Bradford 1x dye reagent (#500-0205, Bio-Rad, United States). Proteins were separated on an SDS-PAGE gel (Bio-Rad) and transferred to PVDF membranes (Millipore, United States), where they were blocked with 5% skim milk. Then, the membranes were incubated at 4°C overnight with the corresponding primary antibodies and HRP-conjugated secondary antibodies. Specific protein bands were visualized using Western Bright Sirius chemiluminescent HRP substrate (Pierce, United States) with a ChemiDoc XRS image detector (Bio-Rad). The following antibodies were used in this experiment: rabbit polyclonal anti-LC3B (L7543, Sigma-Aldrich, United States), anti-SQSTM1/p62 (5114, Cell Signaling Technology, United States), anti-Lamp2 (PA1-655, Invitrogen, United States), anti-Lamp1 (ab24170, Abcam), anti-ATG5 (12994, Cell Signaling Technology, United States), rabbit monoclonal anti-Cl-M6PR (ab124767, Abcam), and anti-CD-M6PR (ab134153, Abcam) and mouse monoclonal anti-beta-actin (ab8226, Abcam), anti-cathepsin D (sc-377124, Sana Cruz Biotechnology).
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