Log In Sign up
The largest database of trusted experimental protocols

Alzet microosmotic pumps model 1002

Manufactured by Durect
Visit Supplier

The ALZET microosmotic pump (Model 1002) is a laboratory equipment designed to provide controlled and continuous delivery of substances. It operates using an osmotic mechanism to release the pre-filled substance at a predetermined rate over an extended period of time.

Automatically generated - may contain errors

Lab products found in correlation

Angii, by Bachem (1 mentions) Ultra sensitive mouse insulin elisa kit, by Crystal Chem (1 mentions) Accu chek aviva, by Roche (1 mentions) Humulin, by Eli Lilly (1 mentions)

Close spelling variants of this product

Alzet model 1002 Microosmotic pump ALZET 1002 Alzet pumps Model 1002

3 protocols using alzet microosmotic pumps model 1002

1

Examining ACE2KO Mice on High-Fat Diet

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Ace2−/y mutant mice (ACE2KO) backcrossed into the C57BL/6 background for at least eight generations were used in the current study (18 (link),21 (link),22 (link)). All experiments were performed in accordance with University of Alberta institutional guidelines, which conform to guidelines published by the Canadian Council on Animal Care and the Guide for the Care and Use of Laboratory Animals published by the U.S. National Institutes of Health (revised 2011). Male wild-type (WT) and ACE2KO mice were fed either a high-fat diet (HFD) (45% kilocalories from fat) or a control diet (CON) (10% kilocalories from fat) from weaning to 6 months of age. ALZET microosmotic pumps (Model 1002; DURECT Corporation) were implanted subcutaneously in ACE2KO-HFD mice to deliver Ang 1-7 (24 μg/kg/h) or saline (control) for 4 weeks (21 (link)). All mice were studied at 6 months of age. Epicardial adipose tissues (EATs) were collected under a stereo microscope after removal of the pericardium and pericardial fat.
Patel V.B., Mori J., McLean B.A., Basu R., Das S.K., Ramprasath T., Parajuli N., Penninger J.M., Grant M.B., Lopaschuk G.D, & Oudit G.Y. (2015). ACE2 Deficiency Worsens Epicardial Adipose Tissue Inflammation and Cardiac Dysfunction in Response to Diet-Induced Obesity. Diabetes, 65(1), 85-95.
+ Open protocol
+ Expand
2

Angiotensin II-Induced Hypertension in GPER Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All procedures were approved by the Tulane University Institutional Animal Care and Use Committee and are in accordance with the NIH Guide for the Care and Use of Laboratory Animals. Heterozygous breeders30 ,31 (link) were used to create homozygous male and female wildtype (wt) and global G protein-coupled estrogen receptor (GPER) knockout (ko) mice that were maintained at Tulane University vivarium, and GPER expression was confirmed by genotyping. Mice were provided with water and food ad libitum in a temperature-controlled room (65-75°F) with a 12 h light to dark cycle. Alzet® micro-osmotic pumps model 1002 (DURECT Corporation, Cupertino, CA) containing Ang II (Bachem Americas Inc, Torrance, CA) were implanted subcutaneously to infuse at a rate of 700 ng/kg/min for two weeks. Mice infused with Ang II that did not reach a systolic blood pressure > 130 mmHg were excluded from the study. Mice were euthanized by isoflurane overdose and exsanguination at 20 ± 3 weeks of age, and carotid arteries were excised for biaxial pressure myography followed by constitutive modeling and histology.
Ogola B.O., Clark G.L., Abshire C.M., Harris N.R., Gentry K.L., Gunda S.S., Kilanowski-Doroh I., Wong T.J., Visniauskas B., Lawrence D.J., Zimmerman M.A., Bayer C.L., Groban L., Miller K.S, & Lindsey S.H. (2021). Sex and the G Protein-Coupled Estrogen Receptor Impact Vascular Stiffness. Hypertension (Dallas, Tex. : 1979), 78(1), e1-e14.
+ Open protocol
+ Expand
3

Continuous Subcutaneous Insulin Delivery

Check if the same lab product or an alternative is used in the 5 most similar protocols
Alzet Micro-osmotic pumps, model 1002 with pumping rate 0.25μl/hr (DURECT Corporation) were filled with Humulin (Eli Lilly) diluted in 1X sterile PBS to allow Insulin release of 0.2 or 0.3U/day. Pumps were implanted subcutaneously under isoflurane anesthesia. The mice were allowed to recover and their plasma glucose was monitored using glucose meter (ACCU-CHEK Aviva, Roche). Plasma insulin levels was measured by ELISA (Crystal Chem, Ultrasensitive mouse insulin ELISA kit, Catalogue #90080, Lot # 16SEUMI411 that detects both human and mouse insulin)
Soundarapandian M.M., Juliana C.A., Chai J., Haslett P.A., Fitzgerald K, & De León D.D. (2020). Activation of Protein Kinase A (PKA) signaling mitigates congenital hyperinsulinism associated hypoglycemia in the Sur1-/- mouse model. PLoS ONE, 15(7), e0236892.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!