Specimens were imaged using an epifluorescence microscope (DM4000; Leica) equipped with DAPI, Endow GFP, and Texas red zero-pixel shift filter sets (Chroma Technology Corp.), a 63× 1.32 NA oil immersion objective, and a camera (CoolSNAP HQ; Roper Industries) controlled by MetaMorph 7. Z stacks were collected at 0.2 μm intervals and deconvolved using AutoDeblur (version X1; Media Cybernetics) for 10 iterations. Line scans (100 pixels wide) were performed using MetaMorph 7. For formalin-fixed, paraffin-embedded specimens stained as part of tissues microarrays, tiled images were collected on a DM4000 microscope equipped with Chroma filter sets (as above), a 20× HC PLAN APO NA 0.7 objective, CoolSNAP HQ2 grayscale CCD (for fluorescence) and Jenoptik ProgRes C10 Plus color CCD (for transmitted light) cameras, and a motorized xyz-stage (Ludl) controlled with custom MetaMorph 7 journals. Images were stitched using MetaMorph 7. Single images of H&E-stained slides were acquired using a MicroPublisher 5.0 CCD camera (Q Imaging) on a Leica DMLB microscope equipped with 10× NA0.25 and 40× NA0.65 HC FL PLAN objectives.
Coolsnap hq2
Manufactured by Jenoptik
The CoolSNAP HQ2 is a high-performance, cooled digital CCD camera designed for scientific imaging applications. It features a high-resolution sensor, advanced cooling technology, and robust construction to capture high-quality images with low noise and high sensitivity.
Automatically generated - may contain errors
2 protocols using coolsnap hq2
1
Epifluorescence Microscopy Imaging Protocol
2
Epifluorescence Microscopy Imaging Protocol
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Specimens were imaged using an epifluorescence microscope (DM4000; Leica) equipped with DAPI, Endow GFP, and Texas red zero-pixel shift filter sets (Chroma Technology Corp.), a 63× 1.32 NA oil immersion objective, and a camera (CoolSNAP HQ; Roper Industries) controlled by MetaMorph 7. Z stacks were collected at 0.2 μm intervals and deconvolved using AutoDeblur (version X1; Media Cybernetics) for 10 iterations. Line scans (100 pixels wide) were performed using MetaMorph 7. For formalin-fixed, paraffin-embedded specimens stained as part of tissues microarrays, tiled images were collected on a DM4000 microscope equipped with Chroma filter sets (as above), a 20× HC PLAN APO NA 0.7 objective, CoolSNAP HQ2 grayscale CCD (for fluorescence) and Jenoptik ProgRes C10 Plus color CCD (for transmitted light) cameras, and a motorized xyz-stage (Ludl) controlled with custom MetaMorph 7 journals. Images were stitched using MetaMorph 7. Single images of H&E-stained slides were acquired using a MicroPublisher 5.0 CCD camera (Q Imaging) on a Leica DMLB microscope equipped with 10× NA0.25 and 40× NA0.65 HC FL PLAN objectives.
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