To prepare samples for photopolymerization, functionalized PCL prepolymers, which were synthesized by reacting PCL diol and triol with acryloyl chloride (Sigma-Aldrich, St. Louis, MO; see Supplemental Materials and Methods ), were dissolved in dioxane (Sigma-Aldrich) to form PCL:dioxane solutions (Ratios of 3:1, 1:1, 1:3). A photoinitiator, 2-Benzyl-2-dimethylamino-1-(4-morpholinophenyl)-butanone-1 (commonly known as Irgacure 369 or I-369, Ciba Specialty Chemicals, BASF, Ludwigshafen, Germany) was added to the solution (at 1, 3, and 5 wt%).
Dioxane
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, France
Dioxane is a laboratory chemical used as a solvent. It has a cyclic structure and a molecular formula of C4H8O2. Dioxane is commonly used in various chemical applications, including as a solvent for resins, fats, oils, waxes, and other organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Merck Group (16 mentions)
Methanol, by Merck Group (13 mentions)
Hydrochloric acid (hcl), by Merck Group (12 mentions)
Dichloromethane, by Merck Group (9 mentions)
Chloroform, by Merck Group (9 mentions)
Toluene, by Merck Group (9 mentions)
Acetone, by Merck Group (9 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (9 mentions)
Tetrahydrofuran, by Merck Group (8 mentions)
Acetonitrile, by Merck Group (8 mentions)
Sodium hydroxide (naoh), by Merck Group (8 mentions)
Trifluoroacetic acid, by Merck Group (7 mentions)
Sulfuric acid, by Merck Group (6 mentions)
Ethyl acetate, by Merck Group (6 mentions)
Dimethylformamide, by Merck Group (5 mentions)
Triethylamine, by Merck Group (4 mentions)
Acetic acid, by Merck Group (4 mentions)
Hexane, by Merck Group (4 mentions)
N hexane, by Merck Group (4 mentions)
Xylene, by Merck Group (3 mentions)
79 protocols using dioxane
1
Photopolymerization of Functionalized PCL
2
Cresyl Violet Staining of Lumbar Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lumbar sections were briefly rehydrated in Milli-Q and were subjected to cresyl violet staining. Sections were next immersed in differentiating solution (1:1 absolute ethanol and dioxane), followed by dehydration in dioxane (Merck-Millipore, Sigma) and tissue clearance in xylene (Merck-Millipore, Sigma). Finally, slides were mounted with DPX (dibutylpthalate polystere xylene) (Sigma) and were dried for 24 h in dark boxes. Images were captured on a Leica DMRXA2 microscope at ×5 and ×40 oil magnifications.
3
Pyrene-based Polymer Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Materials: 1-Acetylpyrene (Frinton labs, Hainesport, NJ, USA), morpholine (Alfa Aesar, Haverhill, MA, USA, +99%), sulfur, (Sigma Aldrich, St. Louis, MO, USA, reagent grade, purified by refining), p-toluene sulfonic acid, (Acros but distributed by Fisher Scientific: Pittsburgh, PA, USA; monohydrate), 20–25% aminopropylmethylsiloxane—dimethylsiloxane co-polymer (Gelest, Morrisville, PA, USA, AMS-1203; Mw: 20,000), acetic anhydride (Sigma, ≥99%), potassium bicarbonate (Sigma, 99%), 1,1′-carbonyldiimidazole (Fisher, Hampton, NH, USA, 97%, CDI) were used as received. N,N′-Dicyclohexylcarbodiimide (DCC) and N,N-dimethylpyridin-4-amine (DMAP) were purchased from Aldrich. Dichloromethane (Fisher, 99%) and dimethylsulfoxide (Fisher, 99%; DMSO) were dried over 4 Å molecular sieves for 3 days before use. Tetrahydrofuran (THF, Caledon, ON, Canada, distilled in glass), N,N-dimethylformamide (DMF, Sigma, ≥99.8), and dioxane (Sigma, ≥99.5%) were used for the fluorescence measurements.
4
Fabrication of Cellulose-Polymer Composites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Microcrystalline cellulose (MCC) (~20 micron, powder) and polyvinylpyrrolidone (powder, average Mw 40,000) (Figure 1 ) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Propanol, dioxane, and chloroform of analytical grade were purchased from Sigma-Aldrich Rus and were used without further purification (Moscow, Russia). Deionized water (18 mΩ, Millipore systems) was used throughout the experiment. Sulfuric acid (chemically pure, GOST 4204-77) was purchased from Chimmed (Moscow, Russia). Potassium bromide (FT-IR grade, >99%) was purchased from Sigma-Aldrich Rus (Moscow, Russia).
5
Synthesis and Characterization of Surfactant-based Crude Oil Emulsions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Curcumin (CRC) (≥99.5%), 1,3-propanesultone (≥99%), potassium tertiary butoxide (t-BuOK) (≥98%), glycidyl 4-nonylphenyl ether (GNE), ethanolamine (EA) (≥98%), diethylene glycol (DEG) (99%), bis (2-chloroethyl) ether (BCE) (≥99%), ethanol absolute (≥99.8%), xylene, dimethylformamide (DMF), and dioxane were purchased from Sigma-Aldrich Co (Missouri, MO, USA). The crude oil was obtained from Aramco Co. Saudi Arabia, and its specifications were fully reported in our previous work [17 (link)]. The brine solution (35 g/L) was prepared in the laboratory using sodium chloride and distilled water.
6
SARS-CoV-2 Spike Protein and Antibody Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
40592-V02H SARS-CoV-2 (2019-nCoV) Spike RBD-Fc Recombinant Protein, 40592-V08B SARS-CoV-2 (2019-nCoV) Spike RBD-His, 40590-V02H SARS-CoV-2 (2019-nCoV) Spike S2 ECD-fc, 40592-V08H82 SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His, 40592-V08H84 SARS-CoV-2 (2019-nCoV) Spike RBD(E484K)-His, 40591-V02H SARS-CoV-2 (2019-nCoV) Spike S1-Fc, 10108-H08H ACE2 Protein, Human-His, 40592-MM57 SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody (Mouse mAb), 40069-MM23 MERS-CoV Spike Protein S1 Antibody, Mouse mAb, 40150-D002 SARS-CoV-2 (2019-nCoV) Spike Antibody Chimeric mAb were purchased from SinoBiological (China). They were first reconstituted in 500 μl of nanopure H2O and then diluted in 1 : 50 human serum (Sigma Aldrich, Ireland) to perform the experiments. Dithiobis(succinimidyl undecanoate) (DSU)33 (link) was purchased from Dojindo Molecular Technologies, Japan. Phosphate buffered saline (PBS), 10 mM, pH 7.4 and 1,4-Dioxane (99.8%) and NTA-SAM were purchased from Sigma Aldrich, Ireland.
7
Cytotoxicity Evaluation of Novel Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Propylene carbonate, dioxane, sulfuric acid, hydrochloric acid, sodium hydroxide, salicylic aldehyde, diethylmalonate, piperidine, N, N-diethyl salicylic aldehyde, ethanol, glucose, fructose, sodium citrate, sodium acetate, ammonium chloride, ammonia, potassium dichromate, deuterated methanol were purchased from Sigma Aldrich (Poznań, Polska). Albumin was obtained from egg white. Lignin was derived from dried miscant plant via extraction. XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay, DMEM without phenol red with high glucose, penicillin/streptomycin, trypsin, phosphate buffer, L929 mouse fibroblasts were purchased from Sigma Aldrich (Poznań, Polska). Sterile filters 0.22 µm were purchased from Bionovo (Legnica Poland) and regenerated cellulose membranes for dialysis (MWCO = 1000 Da) were obtained from VWR International (Gdańsk, Poland). Multiwhole plates (96 wholes) were purchased from Genoplast, Rokocin, Poland.
8
Synthesis of Lithium-Based Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cobalt chloride (CoCl2) (dry or hydrated with two H2O), lithium phenoxide (LiOPh) in tetrahydrofuran (THF), lithium iso-propoxide (LiOiPr) in THF, ethanol (technical grade and analytical grade), tetramethylethylenediamine ( TMEDA), dioxane, dimethoxyethane (DME), pyridine (Py), heptane and micron-sized HT-LiCoO2 were purchased from Sigma-Aldrich (Switzerland). Lithium tert-butoxide (LiOtBu) in THF, lithium methoxide (LiOMe) in methanol, lithium ethoxide (LiOEt) in THF and THF (dry and over molecular sieves) were purchased from Acros Organics (Belgium). Deionized water was produced in house by double distillation.
9
HPLC Purification of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A 3 min gradient of 4% to 100% acetonitrile (containing 0.025% trifluoroacetic acid) in water (containing 0.05% trifluoroacetic acid) was used with a 4.5 min run time at a flow rate of 1 mL/min. A Phenomenex Gemini Phenyl column (3 μm, 3 × 100 mm) was used at a temperature of 50 °C.
All air or moisture sensitive reactions were performed under positive pressure of nitrogen or argon with oven-dried glassware. Anhydrous solvents and bases such as dichloromethane, N,N-dimethylforamide (DMF), acetonitrile, ethanol, DMSO, dioxane, DIPEA (diisopropylethylamine), and triethylamine were purchased from Sigma-Aldrich. Palladium catalysts were purchased from Strem Chemicals and used as such. Preparative purification was performed on a Waters semi-preparative HPLC system using a Phenomenex Luna C18 column (5 μm, 30 × 75 mm) at a flow rate of 45 mL/min (For all compounds tested, the HPLC traces were provided inSupplementary Figure S5 ). The mobile phase consisted of acetonitrile and water (each containing 0.1% trifluoroacetic acid). A gradient of 10% to 50% acetonitrile over 8 min was used during the purification. Fraction collection was triggered by UV detection (220 nm). Analytical analysis was performed on an Agilent LC/MS (Agilent Technologies, Santa Clara, CA).
![]()
All air or moisture sensitive reactions were performed under positive pressure of nitrogen or argon with oven-dried glassware. Anhydrous solvents and bases such as dichloromethane, N,N-dimethylforamide (DMF), acetonitrile, ethanol, DMSO, dioxane, DIPEA (diisopropylethylamine), and triethylamine were purchased from Sigma-Aldrich. Palladium catalysts were purchased from Strem Chemicals and used as such. Preparative purification was performed on a Waters semi-preparative HPLC system using a Phenomenex Luna C18 column (5 μm, 30 × 75 mm) at a flow rate of 45 mL/min (For all compounds tested, the HPLC traces were provided in
10
Extraction and Characterization of Bacterial Lipopeptides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ITZ was generously donated by Ferozsons Laboratories, Nowshera, Pakistan. 1,4 Dioxane, methanol, chloroform, dimethyl sulfoxide (DMSO) and methyl propane sulfonic acid were purchased from Sigma Aldrich® Biochemie GmbH, Hamburg, Germany. Double distilled water was obtained from the distillation unit installed in the laboratory, faculty of pharmacy, Bahauddin Zakariya University, Multan, and SA was extracted from bacterial lipopeptides.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!