Jurkat

Manufactured by Leibniz Institute DSMZ

Sourced in Germany

Jurkat is a T cell line derived from a human T cell leukemia. It is a commonly used cell line in immunological research and serves as a model for human T cell function and signaling.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Jurkat cells (6 citations)

44 protocols using jurkat

Comprehensive Cell Line Culturing Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell lines were cultured in a humidified incubator at 37°C with 5% CO₂. The following cell lines were obtained from ATCC: HEK 293 T, WI-38, WI-38 VA 13, Daudi, EB1, HS604T, HS616T, HuT 102, MC116, Namalwa, H1963, H196, H209, H526, H524, H82, H69, Raji, SW1271, and TO175T. DEL, L428, SU-DHL-10, WSU-DLCL2, Jurkat, DND41 and SR768 were purchased from DSMZ. A4/Fuk was obtained from JCRB Cell Line Bank. OCI-Ly3 was a kind gift from Dr. Mark Minden (University Health Network Toronto). The human lung fibroblast cell lines WI-38 and WI-38 VA13 were routinely cultured in EMEM supplemented with 10% Fetal Bovine Serum (FBS). SCLC cell lines H1963, H196, SW1271, H209, H526, H524, H82 and H69 were maintained in RPMI 1640 with 10% FBS. The lymphoma cell lines EB1, Daudi, Raji, A4/Fukuda, Jurkat, DND41, WSU-DLCL2, DEL, HUT102, Namalwa and L428 were cultured in RPMI 1640 with 10% FBS. MC116 and SU-DHL-10 cells were maintained in RPMI 1640 with 20% FBS. OCI-Ly3 cells were cultured in IMDM with 20% FBS. The SR786 cell line was cultured in RPMI 1640 with 15% FBS. HS604T cells were maintained in DMEM with 10%FBS and 2 mM Glutamine. HS616T and TO175T cell lines were cultured in DMEM with 10% FBS. HEK 293 T cells were maintained in DMEM with 10% FBS. All cell lines were maintained with a cocktail of penicillin and streptomycin (Gibco).

Zhou Z., Patel M., Ng N., Hsieh M.H., Orth A.P., Walker J.R., Batalov S., Harris J.L, & Liu J. (2014). Identification of synthetic lethality of PRKDC in MYC-dependent human cancers by pooled shRNA screening. BMC Cancer, 14, 944.

+ Open protocol

+ Expand

Cell Culture Protocol for Lymphoid Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPB-ALL and JURKAT were obtained from DSMZ. DAUDI and RAJI were obtained from American Type Culture Collection. Cells were cultured in standard conditions in RPMI media supplemented with 10% FBS and 1% Penicillin/Streptomycin.

Herranz D., Ambesi-Impiombato A., Palomero T., Schnell S.A., Belver L., Wendorff A.A., Xu L., Castillo-Martin M., Llobet-Navás D., Cardo C.C., Clappier E., Soulier J, & Ferrando A.A. (2014). N-Me, a long range oncogenic enhancer in T-cell acute lymphoblastic leukemia. Nature medicine, 20(10), 1130-1137.

+ Open protocol

+ Expand

Cell Line Cultivation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

PF-382, TALL-1, HPB-ALL, and Jurkat were purchased from DSMZ. CCRF-CEM and HEK293T cell lines were obtained from ATCC.
PF-382, TALL-1, CCRF-CEM, and Jurkat, were cultured in RPMI 1640 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% fetal bovine serum (Lonza Group Ltd., Basel, Switzerland), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Thermo Fisher Scientific), and maintained at 37 °C in a 5% CO₂ atmosphere.
The HPB-ALL cell line was cultured in RPMI 1640 supplemented with 20% fetal bovine serum.
The HEK293T cell line was cultured in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Riillo C., Caracciolo D., Grillone K., Polerà N., Tuccillo F.M., Bonelli P., Juli G., Ascrizzi S., Scionti F., Arbitrio M., Lopreiato M., Siciliano M.A., Sestito S., Talarico G., Galea E., Galati M.C., Pensabene L., Loprete G., Rossi M., Ballerini A., Gentile M., Britti D., Di Martino M.T., Tagliaferri P, & Tassone P. (2022). A Novel Bispecific T-Cell Engager (CD1a x CD3ε) BTCE Is Effective against Cortical-Derived T Cell Acute Lymphoblastic Leukemia (T-ALL) Cells. Cancers, 14(12), 2886.

+ Open protocol

+ Expand

Evaluating CAR-DOT Cytotoxicity and Cytokine Production

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cell lines MOLM13, THP-1 and Jurkat were purchased from DSMZ (Germany) and expanded according to DSMZ recommendations. Target cells (cell lines and primary AML blasts) were labeled with 3 µM eFluor 670 (eBioscience) and incubated with CAR-DOTs or mock-DOTs at different Effector:Target (E:T) ratios for the indicated time periods in complete DOT media. CAR-DOT-mediated cytotoxicity was determined by analyzing the residual alive (7-AAD^–) eFluor 670⁺ target cells at each time point and E:T ratio. Absolute cell counts were determined using Trucount absolute count beads (BD Biosciences).28 (link) Cytokine production was measured by G-Series Human Cytokine Antibody Array 4000 from RayBiotech in supernatants harvested after 48 hours-incubation CAR-DOT/mock-DOT (four donors) with two primary AML samples.

Sánchez Martínez D., Tirado N., Mensurado S., Martínez-Moreno A., Romecín P., Gutiérrez Agüera F., Correia D.V., Silva-Santos B, & Menéndez P. (2022). Generation and proof-of-concept for allogeneic CD123 CAR-Delta One T (DOT) cells in acute myeloid leukemia. Journal for Immunotherapy of Cancer, 10(9), e005400.

+ Open protocol

+ Expand

Cell Line Validation and Mycoplasma Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols

A4573 were a gift from the Children’s Hospital Los Angeles, United States. HEK293T, Kelly, Jurkat, A204 and SUP-B15 were purchased from DSMZ (Braunschweig, Germany). Short tandem repeat (STR) profiling was used to confirm the identity of all cell lines. All cell lines were regularly checked by PCR to exclude mycoplasma contamination.

Altvater B., Kailayangiri S., Spurny C., Flügge M., Meltzer J., Greune L., Urban K., Schwöppe C., Brand C., Schliemann C., Hintelmann H., Harrach S., Hartmann W., Abken H., Kuehle J., Schambach A., Görlich D., Berdel W.E, & Rossig C. (2023). CAR T cells as micropharmacies against solid cancers: Combining effector T-cell mediated cell death with vascular targeting in a one-step engineering process. Cancer Gene Therapy, 30(10), 1355-1368.

+ Open protocol

+ Expand

Culturing Hematological Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Four hematological cell lines (ML2—acute myeloid leukemia; Jurkat—acute lymphoblastic leukemia; Namalwa—Burkitt lymphoma; and RPMI8226—multiple myeloma) were purchased from DSMZ (German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) or ATCC.
All cells were cultured in RPMI medium (Invitrogen AG, 61870-01) supplemented with 10% heat inactivated fetal calf serum (Amimed, 2-01F30-I) and 1% penicillin/streptomycin at 37 °C (Amimed, 4-01F00-H) in a humidified atmosphere of 95% air and 5% CO₂.

Biniecka P., Matsumoto S., Belotti A., Joussot J., Bai J.F., Majjigapu S.R., Thoueille P., Spaggiari D., Desfontaine V., Piacente F., Bruzzone S., Cea M., Decosterd L.A., Vogel P., Nencioni A., Duchosal M.A, & Nahimana A. (2023). Anticancer Activities of Novel Nicotinamide Phosphoribosyltransferase Inhibitors in Hematological Malignancies. Molecules, 28(4), 1897.

+ Open protocol

+ Expand

Culturing Human Leukemia Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human B-ALL cell lines SEM, RS4;11 and the T-ALL cell lines Jurkat and MOLT4 were purchased from DSMZ (Braunschweig, Germany) and cultured according to manufacturer’s protocol. The corresponding medium was supplemented with 10% heat-inactivated fetal bovine serum (PAA, Pasching, Austria) and 1% penicillin and streptomycin (Biochrom AG, Berlin, Germany). The MOLT4 cells were cultured with medium supplemented with 20% heat-inactivated fetal bovine serum. All cells were maintained at 37°C in 5% CO₂.

Kretzschmar C., Roolf C., Langhammer T.S., Sekora A., Pews-Davtyan A., Beller M., Frech M.J., Eisenlöffel C., Rolfs A, & Junghanss C. (2014). The novel arylindolylmaleimide PDA-66 displays pronounced antiproliferative effects in acute lymphoblastic leukemia cells. BMC Cancer, 14, 71.

+ Open protocol

+ Expand

Cell Line Assay and T Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

SEM, REH, RAJI, Jurkat, and K562 cell lines were obtained from DSMZ. KOPN8, KOBP26, and NALM6 cell lines were obtained from ATCC. Mesenchymal stem cells (MSCs) were obtained from Shanghai Nerostem Tech. CD3+ T cells were isolated using EasySep Human T Cell Isolation Kit (STEMCELL Technologies) and then cultured in CTS T Cell Expansion medium (Thermo) containing 10% fetal bovine serum and 100 IU/ml human IL-2 (PeproTech). The CellTiter 96 MTS assay (Promega) was used to determine cell viability and proliferation.

Li D., Wang W., Xie S., Ge M., Wang R., Xu Q., Sun Y., Zhu J, & Liu H. (2022). A T-cell independent universal cellular therapy strategy through antigen depletion. Theranostics, 12(3), 1148-1160.

+ Open protocol

+ Expand

Cell Culture Maintenance Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Jurkat (ATCC Cat# TIB-152, RRID : CVCL_0367), HeLa (ATCC Cat# CCL-2.2, RRID : CVCL_0058), NOMO-1 (DSMZ Cat# ACC-542, RRID : CVCL_1609), K562 (ATCC Cat# CCL-243, RRID : CVCL_0004) and U87 (ATCC Cat# HTB-14, RRID : CVCL_0022) cell lines were all acquired from ATCC and cultured as recommended by the supplier. LAN-1 cell line (DSMZ Cat# ACC-655, RRID : CVCL_1827) was acquired from DSMZ and cultured as recommended by the supplier. Cell lines were screened monthly for mycoplasma contamination Briefly, Jurkat, K562 and NOMO-1 cells were grown in 10% FBS-supplemented RPMI1640 (Sigma Aldrich) suspension culture and kept at <1x10⁶/mL density. LAN-1, HeLa and U87 cell lines were grown in 10% FBS-supplemented DMEM (Thermo Fisher) adherent culture and split regularly at around 80-90% confluency using trypsin (Thermo Fisher)-based disaggregation, to avoid overgrowth.

Ferry G.M., Agbuduwe C., Forrester M., Dunlop S., Chester K., Fisher J., Anderson J, & Barisa M. (2022). A Simple and Robust Single-Step Method for CAR-Vδ1 γδT Cell Expansion and Transduction for Cancer Immunotherapy. Frontiers in Immunology, 13, 863155.

+ Open protocol

+ Expand

Cell Line Culture and Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human cell lines SUDHL-1, Karpas-299, HPB-ALL, DND41 and Jurkat were purchased from the DSMZ and were cultured in RPMI1640 supplemented with 10% FCS. The COST and PIO ALCL cell lines were developed in the Lamant laboratory and were grown in ISCOVE medium (Invitrogen, Cergy Pontoise, France) supplemented with 10% FCS. All the cell lines were subject to quarterly mycoplasma testing and are not on the ICLAC and NCBI Biosample misidentified cell list.

Malcolm T.I., Villarese P., Fairbairn C.J., Lamant L., Trinquand A., Hook C.E., Burke G.A., Brugières L., Hughes K., Payet D., Merkel O., Schiefer A.I., Ashankyty I., Mian S., Wasik M., Turner M., Kenner L., Asnafi V., Macintyre E, & Turner S.D. (2016). Anaplastic large cell lymphoma arises in thymocytes and requires transient TCR expression for thymic egress. Nature Communications, 7, 10087.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!