Agilent open lab a 02.02 cds chemstation

The contents of artemisinin in QHP were determined by HPLC. Quantitative analysis of artemisinin in QHP was performed on the Agilent 1290 Infinity apparatus comprising two solvent delivery systems and a photodiode array detector (Agilent, USA). The column was an Agilent ZORBAX SB-C18 chromatographic column (4.6 mm × 250 mm, 5.0 μm). The mobile phase consisted of acetonitrile and H₂O (60: 40), and the pH value was 6.8–7.2. Reagents were filtered through a Millipore 0.45 mm filter and degassed prior to use. The entire run was carried out by gradient elution at a flow rate of 1.0 mL/min; the detection wavelength was set at 210 nm; the column was maintained at 35°C, and the injection volume was 10 μL. Data collection and quantification were performed with Agilent Open LAB A.02.02 CDS ChemStation (Agilent, USA). The peak of artemisinin was identified by comparison with chemical standards.

The contents of β-dichroine and α-dichroine in UEDR were determined by HPLC. Quantitative analysis of two components in UEDR was performed on a Agilent apparatus (1290 infinity, two solvent delivery systems, and a Photodiode Array detector, Agilent, USA), SB-C₁₈ chromatographic column (4.6 mm × 250 mm, 5 µm, Agilent, USA). The mobile phase consisted of acetonitrile and 0.3% (v/v) glacial acetic acid (30:70), and the pH value adjusted to 5.2–6.2 with triethylamine, filtered through a millipore 0.45 mm filter and degassed prior to use. The injection volume was 10 µL. The flow rate was 1.0 mL/min. The detection wavelength for HPLC analysis was set at 265 nm. The column was maintained at 25 °C. Data collection and quantification were performed with Agilent Open LAB A.02.02 CDS ChemStation (Agilent, USA). The peaks of β-dichroine and α-dichroine were identified by comparison with chemical standards.