Log In Sign up
The largest database of trusted experimental protocols

Taqman fast mix 2x

Manufactured by Thermo Fisher Scientific
Visit Supplier

TaqMan Fast Mix 2x is a ready-to-use, 2X concentrated, thermostable master mix for real-time PCR. It is designed for fast and sensitive quantification of DNA and RNA targets.

Automatically generated - may contain errors

Lab products found in correlation

Rneasy plus mini kit, by Qiagen (3 mentions) Quantstudio 7 flex real time pcr system, by Thermo Fisher Scientific (3 mentions) High capacity cdna rt kit, by Thermo Fisher Scientific (3 mentions) Mirneasy mini kit, by Qiagen (1 mentions)

Close spelling variants of this product

TaqMan Fast Universal PCR Master Mix (2X) (63 citations) TaqMan® Fast Advanced Master Mix (2X) (12 citations) TaqMan™ Fast Universal PCR Master Mix (2X) no AMPERASE™ UNG (7 citations) TaqMan Fast Universal Master Mix 2X (6 citations) Fast Taqman mix (4 citations) TaqMan Fast Universal Master Mix (2X) No AmpErase UNG (4 citations)

3 protocols using taqman fast mix 2x

1

RNA Extraction and qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed with cold PBS, and RNA extraction was done using the RNeasy PLUS Mini Kit (Qiagen, Germantown, MD). cDNA was prepared using the High Capacity cDNA RT Kit (Applied Biosystems, Foster City, CA). TaqMan Fast Mix 2x (Applied Biosystems) was used to prepare qPCR along with the primer/probe sets described in Key Resources Table. Reactions were run on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). All CT values were normalized to Gapdh, and the ΔΔCT method was used to calculate fold changes.
Pandey V., Fleming-Martinez A., Bastea L., Doeppler H.R., Eisenhauer J., Le T., Edenfield B, & Storz P. (2021). CXCL10/CXCR3 signaling contributes to an inflammatory microenvironment and its blockade enhances progression of murine pancreatic precancerous lesions. eLife, 10, e60646.
+ Open protocol
+ Expand
2

Quantitative RT-PCR Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA extraction was performed using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) and miRNeasy Mini Kit (Qiagen, Hilden, Germany), and cDNA was prepared using the High-Capacity cDNA RT Kit (Applied Biosystems, Waltham, MA). TaqMan Fast Mix 2x (Applied Biosystems) was used to prepare qPCR reactions with the TaqMan primer/probe sets noted in the key resources table. Samples were run on a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). For analyses all CT values were normalized to Gapdh and fold changes were calculated using the ΔΔCT method.
Liou G.Y., Fleming Martinez A.K., Döppler H.R., Bastea L.I, & Storz P. (2023). Inflammatory and alternatively activated macrophages independently induce metaplasia but cooperatively drive pancreatic precancerous lesion growth. iScience, 26(6), 106820.
+ Open protocol
+ Expand
3

Quantitative PCR Analysis of Murine M2 Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
For live cells, RNA was extracted with the RNeasy PLUS Mini Kit (Qiagen, Germantown, MD). For fixed cells, RNA was extracted with Arcturus Paradise Plus RNA Extraction and Isolation Kit (Applied Biosystems, Foster City, CA) and then amplified with Arcturus Paradise Plus RNA Amplification Kit (Applied Biosystems). The High Capacity cDNA RT Kit (Applied Biosystems) was used to convert RNA to cDNA and quantitative PCR was performed using a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). qPCR reactions utilized TaqMan Fast Mix 2x (Applied Biosystems) in conjunction with the following probe/primer sets from Applied Biosystems: Gapdh (Mm99999915_g1), Chil3/Ym1 (Mm00657889_mH), Il10 (Mm01288386_m1), Retnla (Mm0045109_m1), Mrc1 (Mm01329362_m1), Clec10a/Cd301 (Mm00546125_g1), Il1rn (Mm00446186_m1), Il1a (Mm00439620_m1), and Arg1 (Mm00475988_m1). Calculation of mRNA abundance was via the ΔΔCT method and normalization was to Gapdh.
Bastea L.I., Liou G.Y., Pandey V., Fleming A.K., von Roemeling C.A., Doeppler H., Li Z., Qiu Y., Edenfield B., Copland J.A., Tun H.W, & Storz P. (2019). Pomalidomide alters pancreatic macrophage populations to generate an immune-responsive environment at precancerous and cancerous lesions. Cancer research, 79(7), 1535-1548.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!