The chemically synthesized miR-125a mimic, miR-125a inhibitor, small interfering RNA (siRNA) of STAT3, STAT3 overexpression vector, and related negative controls (NC) were purchased from RiboBio (Guangzhou, China). Lipofectamine 2000 (Invitrogen, USA) was applied for transfection.
Mir 125a inhibitor
Manufactured by RiboBio
Sourced in China
The MiR-125a inhibitor is a laboratory reagent designed to target and inhibit the expression of the microRNA miR-125a. It can be used by researchers to study the biological functions and regulatory roles of miR-125a in various cellular and molecular processes.
Automatically generated - may contain errors
3 protocols using mir 125a inhibitor
1
Synthetic miR-125a modulates STAT3 signaling
2
Plasmid Construction and Cell Transfection
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To construct plasmids expressing HAX1, the full-length human HAX1 sequence was synthesized by Guangzhou RiboBio Co., Ltd. and ligated into the pcDNA3.1 plasmid (cat. no. V79020; Thermo Fisher Scientific, Inc.). For cell transfection, 2 mg/ml HAX1 vector, 50 pmol/ml miR-125a mimics (cat. no. miR10000443-1-5; Guangzhou RiboBio Co., Ltd.), 50 pmol/ml mimics negative control (mimics NC; cat. no. miR1N0000001-1-5; Guangzhou RiboBio Co., Ltd.), 50 pmol/ml miR-223 mimics (cat. no. miR10000280-1-5; Guangzhou RiboBio Co., Ltd.), 50 pmol/ml miR-125a inhibitor (cat. no. miR20000443-1-5; Guangzhou RiboBio Co., Ltd.), 50 pmol/ml inhibitor NC (cat. no. miR2N0000001-1-5; Guangzhou RiboBio Co., Ltd.), small interfering RNA (siRNA) against HAX1 (siHAX1; cat. no. siG000010456A-1-5; Guangzhou RiboBio Co., Ltd.) and siRNA negative control (siNC; cat. no. siN0000002-1-5, Guangzhou RiboBio Co., Ltd.) were transfected into SK-Hep1 and SNU-387 cells. Cell transfection was conducted using Lipofectamine™ 2000 (cat. no. 11668; Invitrogen; Thermo Fisher Scientific, Inc.). In addition, untreated cells were used as control. SNU-387 cells transfected with pcDNA3.1 empty plasmid were used as the NC group, while SK-Hep1 cells transfected with siRNA negative control used as the siNC group.
3
Modulation of Hepatocyte Proliferation
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Treatments of this part were conducted as our previously described [61 (link)]. Briefly, BRL-3A cells were obtained from cell bank of the School of Basic Medicine of Peking Union Medical College (Beijing, China). The cells had been kept in a high glucose DMEM complete medium, supplemented with 10% fetal bovine serum (FBS), 1% penicillin and 1%streptomycin in a concentration of 5% CO2 incubator at 37 °C. miR-125a mimics (Ribobio, Guangzhou, China), miR-125a inhibitor (Ribobio, China), or their negative controls handled BRL-3A for 48 h, respectively. To further investigate the role of STAT3 in hepatocyte proliferation, cells were transfected with siRNA-STAT3 (siRNA1, 2, 3, Ribobio, China) or negative control for 48 h, respectively.
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