Ibrs 2

Manufactured by Thermo Fisher Scientific

Sourced in United States

The IBRS-2 is a compact, benchtop centrifuge designed for routine sample separation in a laboratory setting. It provides reliable performance with a maximum rotational speed of 6,000 rpm and a maximum relative centrifugal force of 3,345 x g. The unit features a simple user interface and an automatic rotor identification system.

Automatically generated - may contain errors

Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Ccl 33, by American Type Culture Collection (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Hek293, by American Type Culture Collection (1 mentions) Tib 202, by American Type Culture Collection (1 mentions) Thp 1, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions)

2 protocols using ibrs 2

Cell Culture Conditions for Cell Lines

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Porcine kidney (PK-15) (ATCC#CCL-33), human embryonic kidney 293 (HEK293) (ATCC #CRL-1573), human monocytic cell line (THP-1) (ATCC #TIB-202), and Instituto Biologico-Rim Suino-2 (IBRS-2) (ATCC #CRL-1835) cells were purchased from the American Type Culture Collection (USA). PK-15 and IBRS-2 cells were cultured in minimum essential medium (MEM, Gibco, USA) supplemented with 10% heat-inactivated FBS, penicillin (100 U/mL), and streptomycin sulfate (100 μg/mL). Human embryonic kidney 293 (HEK293) cells were cultured in DMEM (Gibco, USA) supplemented with 10% heat-inactivated FBS, penicillin (100 U/mL), and streptomycin sulfate (100 μg/mL). The THP-1 human monocytic cell line was cultured in RPMI 1640 (Gibco, USA) supplemented with 10% heat-inactivated FBS, penicillin (100 U/mL), and streptomycin sulfate (100 μg/mL). All cells were maintained at 37°C with 5% CO₂.

Choudhury S.M., Ma X., Zeng Z., Luo Z., Li Y., Nian X., Ma Y., Shi Z., Song R., Zhu Z., Cao W., Pei J, & Zheng H. (2022). Senecavirus a 3D Interacts with NLRP3 to Induce IL-1β Production by Activating NF-κB and Ion Channel Signals. Microbiology Spectrum, 10(2), e02097-21.

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FMDV Isolation and Titration Protocol

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The FMDVs (O/MYA98/BY/2010 and A/GD/MM/2013) were preserved and provided by OIE/National Foot-and-Mouth Disease Reference Laboratory of China. Swine kidney cells (IBRS-2) were purchased from CCTCC (China Center for Type Culture Collection) and then maintained in our laboratory. IBRS-2 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Grand Island, NY) containing 10% fetal bovine serum (FBS) at 37 °C and 5% CO 2 . Escherichia coli (E. coli) Rosetta (DE3) Lys cells were used for expression of PoIFN-δ8. The TCID 50 of FMDV was calculated according to the Reed and Muench method [12] .

Li S.F., Shao J.J., Zhao F.R., Gong M.J., Xie Y.L., Chang H.Y, & Zhang Y.G. (2018). Antiviral activity of porcine interferon delta 8 against foot-and-mouth disease virus in vitro. International immunopharmacology, 59.

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