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Mouse monoclonal anti human prb

Manufactured by BD

Mouse monoclonal anti-human pRB is a laboratory reagent used for the detection and analysis of the retinoblastoma protein (pRB) in human samples. This antibody specifically binds to the pRB protein, which is a key regulator of cell cycle progression. The core function of this product is to provide a tool for researchers to study the expression and function of pRB in various experimental systems.

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2 protocols using mouse monoclonal anti human prb

1

Western Blot Analysis of Cellular Proteins

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Total cell extracts were obtained by lysing the cells directly using 2× SDS-PAGE sample buffer. Western blotting and processing were then performed as described previously [35 (link)]. The following antibodies were used: mouse monoclonal anti-human pRB (BD Pharmingen); rabbit polyclonal anti-MMP2 and MMP9 (Abcam); rabbit monoclonal p21 (12D1), rabbit polyclonal phospho-Akt (Ser473), rabbit monoclonal pan Akt (Cell Signaling), mouse monoclonal anti-p53 (DO-1), mouse monoclonal anti-β-actin, mouse monoclonal anti-SAP97 (2D11) (Dlg), rabbit polyclonal p130 (C-20) and p107 (C-18), mouse monoclonal p-ERK1/2 (12D4), and ERK1/2 (C-9) were from Santa Cruz.
Immunoblots were developed using Clarity™ Western ECL Substrate (Bio-Rad) and images were captured using the ChemiDoc™ Imaging System (Bio-Rad). Protein band intensities were quantified using ImageJ and normalized with the levels of β-actin, which serves as a loading control.
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2

Antibody Detection in Cell Lines

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The following antibodies were used: mouse monoclonal anti-human pRB (BD Pharmingen), mouse monoclonal anti-p84 (5E10; Abcam), mouse monoclonal anti-α-tubulin (T6199; Sigma). The following antibodies were purchased from Santa Cruz Biotechnology: mouse monoclonal anti-p53 (DO-1), mouse monoclonal anti-α-actinin (H-2), mouse monoclonal anti-Dlg (2D11), goat polyclonal anti-Scribble (C-20), goat polyclonal anti-TIP2 (N-19), rabbit polyclonal anti-E-cadherin (H-108) and mouse monoclonal anti-vimentin (V-9). The following antibodies were from Cell Signaling Technology: rabbit polyclonal anti-PDK1 (3062), rabbit monoclonal anti-phosphorylated PDK1 (S241) (C49H2), rabbit monoclonal anti-p70 S6 kinase (49D7), mouse monoclonal anti-phosphorylated p70 S6 kinase (T389) (1A5). The mouse monoclonal antibody anti HPV-18 E6 (N-terminus #399) was generated and generously provided by the Arbor Vita Corporation. For detection of HPV-16 E6 expression we used the Arbor Vita E6 detection kit according to the manufacturer's instructions (supplied by AB Analitica srl).
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