Anti cd19 pe cy7

Manufactured by BD

Sourced in United States, Germany

Anti-CD19-PE-Cy7 is a fluorescently labeled monoclonal antibody that binds to the CD19 cell surface antigen. It is designed for use in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Facsdiva software, by BD (4 mentions) Facs lysing solution, by BD (4 mentions) Anti cd45 v500, by BD (3 mentions) Anti cd38 apc, by BD (3 mentions) Anti cd56 pe, by BD (3 mentions) Facscanto 2, by BD (3 mentions) Anti cd38 fitc, by BD (3 mentions) Anti cd3 fitc, by BD (3 mentions) Facscanto 2 flow cytometer, by BD (2 mentions) Lsrii flow cytometer, by BD (2 mentions) Anti hvem alexafluor 647, by BD (2 mentions) Anti cd27 brilliantviolet421, by BioLegend (2 mentions) Anti cd20 pe, by BioLegend (2 mentions) Alexafluor 647 conjugated anti btla, by BioLegend (2 mentions) Quantum alexafluor 647 mesf calibration beads, by Bangs Laboratories (2 mentions) Anti cd38 alexafluor 488, by BioLegend (2 mentions) Anti cd19 apc, by BD (2 mentions) Anti cd3 pacific blue, by BD (2 mentions) Anti cd4 percp cy5, by BD (2 mentions) Trucount tube, by BD (2 mentions)

Spelling variants of this product

Anti-CD19 (105 citations) CD19-PE (90 citations) CD19-PE-Cy7 (83 citations) Anti-CD19-PE (53 citations) PE-Cy7-conjugated anti-CD19 (6 citations) Anti-mouse CD19 PE/Cy7, (2 citations) PE-Cy7-conjugated anti-human CD19 (2 citations)

37 protocols using anti cd19 pe cy7

Multiparametric Flow Cytometry for Myeloma

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bone marrow samples were divided into 2 tubes. The first tube was used to measure the MM burden in bone marrow. Cells were stained with anti-CD45-V500 (662912; BD), anti-CD19-PE-Cy7 (560728; BD), anti-CD138-APC (347193; BD), anti-CD38-V450 (562444; BD) and anti-CD56-APC-Cy7 (362512; BioLegend) for 30 min. After fixation and permeabilization, the cells were then stained with anti-clambda-PE (555797; BD) and anti-ckappa-FITC (555791; BD). Data were collected using FACS Canto II flow cytometer (BD, USA), and a minimum of 1 × 10⁶ cells were acquired per sample. MM cells were analyzed according to the report by the European Myeloma Network [44 (link)]. Minimal residual disease (MRD) was defined according to international consensus guidelines and MRD negativity was defined as less than 0.01% nucleated cells [45 (link)].
The second tube was used to detect BCMA and CD38 expression on MM cells. Cells were stained with anti-CD45-V500 (662912; BD), anti-CD19-PE-Cy7 (560728; BD), anti-CD138-V450 (562935; BD), anti-CD56-PerCP-Cy5.5 (560842; BD), anti-CD38-APC (555462; BD) and anti-BCMA-PE (130-117-544; Miltenyi Biotec). BCMA and CD38 expression was reported as the percentage of positive cells in CD45⁺ CD138⁺ CD19⁻ cells. Data were collected using FACSCanto II flow cytometer (BD, USA), and data were analyzed using FlowJo software (TreeStar, USA).

Mei H., Li C., Jiang H., Zhao X., Huang Z., Jin D., Guo T., Kou H., Liu L., Tang L., Yin P., Wang Z., Ai L., Ke S., Xia Y., Deng J., Chen L., Cai L., Sun C., Xia L., Hua G, & Hu Y. (2021). A bispecific CAR-T cell therapy targeting BCMA and CD38 in relapsed or refractory multiple myeloma. Journal of Hematology & Oncology, 14, 161.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were labelled with flow antibodies for 15 minutes in the dark in PBS containing 2% BSA. Labelled cells were washed twice and resuspended in PBS containing 2% BSA. The prepared samples were analyzed using an LSR-II flow cytometer (BD Bioscience) or sorted using an Aria II cell sorter (BD Bioscience). Anti-CD19-PeCy7 (561739), anti-CD3-PeCy7 (552774), anti-NK1.1-BV421 (562921), anti-NKp46-FITC (560756), anti-NKp46-AF647 (560755), anti-CD49b-PE (553858), anti-CD49a-PerCP-Cy5.5 (564862), anti-CD62L-APC (553152), anti-T-bet-APC (561264), anti-CD45.2-AF700 (560693), anti-CD45.2-FITC (553772), anti-Gata3-AF647 (560068), anti-RORγt-PE (562607), anti-CD127-V450 (561205), anti-CD117-PE (553869), anti-LPAM-1-APC (562376), anti-Flt3-BV421 (566292), anti-Ly-6A/E-APC (565355), and anti-CD122-PE (553362) were purchased from BD Bioscience. Anti-IFN-γ-AF-700 (505823) and anti-CD25-Pacific Blue (102022) were purchased from Biolegend. Anti-CD253-APC (17-5951-82), anti-Eomes-PE (12-4875-82), and anti-CD127-PerCP-Cy5.5 (45-1271-80) were purchased from eBioscience.

Wang Y., Dong W., Zhang Y., Caligiuri M.A, & Yu J. (2018). Dependence of innate lymphoid cell 1 development on NKp46. PLoS Biology, 16(4), e2004867.

+ Open protocol

+ Expand

Quantification of HVEM Expression on B Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated whole CD4⁺ populations or isolated Tfh cells were stained with with AlexaFluor 647-conjugated anti-BTLA (BioLegend, 344520) and compared to Quantum AlexaFluor 647 MESF calibration beads (Bangs Laboratories) by flow cytometry. HVEM levels on B cells were determined by staining PBMCs from whole blood with anti-CD19-PE-Cy7 (BD Biosciences; 560911), anti-CD38-AlexaFluor 488 (BioLegend; 303511), anti-CD27-BrilliantViolet421 (BioLegend; 356417), anti-CD20-PE (BioLegend; 302305), and anti-HVEM-AlexaFluor 647 (BD Biosciences; 564411). B cell subsets were gated as follows: Activated (CD19⁺ CD20⁺ CD27⁺ CD38⁺), Memory (CD19⁺ CD20⁺ CD27⁺ CD38^-), immature/transitional (CD19⁺ CD20⁺ CD27^- CD38⁺), and plasmablasts (CD19⁺ CD20^- CD27⁺ CD38⁺). HVEM intensity on each cell subset was converted to absolute protein numbers by reference to Quantum AlexaFluor 647 MESF calibration beads (Bangs Laboratories). Based on the mean HVEM expression on activated B cells of ∼35,000/cell, minimum surface density was estimated at ∼35 molecules/μm² assuming an upper limit of ∼1000 μm² total area.

Mintz M.A., Felce J.H., Chou M.Y., Mayya V., Xu Y., Shui J.W., An J., Li Z., Marson A., Okada T., Ware C.F., Kronenberg M., Dustin M.L, & Cyster J.G. (2019). The HVEM-BTLA Axis Restrains T Cell Help to Germinal Center B Cells and Functions as a Cell-Extrinsic Suppressor in Lymphomagenesis. Immunity, 51(2), 310-323.e7.

+ Open protocol

+ Expand

Characterization of Peripheral Blood Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood mononuclear cells (PBMCs) were isolated from 12 mL of peripheral venous blood samples collected in K3EDTA tubes (BD vacutainer) by centrifugation using a Ficoll-Paque gradient and were frozen at −80°C. Prior to flow cytometry analyses, cells were thawed, and PBMCs subtypes were distinguished using the following antibodies: anti-CD45-Pacific orange (Invitrogen), anti-CD3-V450 (BD Biosciences), anti-CD4-PE-texas red (Invitrogen), anti-CD8-APC-H7 (BD Biosciences), anti-CD19-PE-Cy7 (BD Biosciences), anti-CD14-PerCP-Cy5 (BD Biosciences), anti-CD16-FITC (BD Biosciences), anti-TLR2-PE (eBiosciences), and anti-TLR4-APC (eBiosciences) (all made in mouse). Data acquisition was performed using a BD LSR II flow cytometer, and the results were analyzed with BD eDiva Software (version 6.1.2, BD Biosciences) and FCS Express 4 Flow Cytometry (De Novo Software).

Drouin-Ouellet J., St-Amour I., Saint-Pierre M., Lamontagne-Proulx J., Kriz J., Barker R.A, & Cicchetti F. (2015). Toll-Like Receptor Expression in the Blood and Brain of Patients and a Mouse Model of Parkinson’s Disease. International Journal of Neuropsychopharmacology, 18(6), pyu103.

+ Open protocol

+ Expand

Splenic Immune Profiling in Infected Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

The spleens of mice were collected at various time points post-infection, as detailed in figure legends. Splenic cells were passed through a 70 mm cell strainer (Fisher Scientific), centrifuged, and then treated with Ammonium-Chloride-Potassium (ACK) red blood cell lysing buffer (Gibco). Following washes, 4 x 10⁶ total spleen cells were incubated with anti-mouse CD16/CD32 (Fc Block, BD Biosciences; Mississauga, ON, Canada) for 15 min on ice according to manufacturer’s instructions. Afterwards, surface staining with the following antibodies: anti-CD95^PE, anti-CD86^biotin, anti-CD19^PE-Cy7, anti-GL-7^AF647 and anti-CD184^BV421 (all BD Biosciences) was performed for 30 min on ice. Following washing, cells were incubated with Brilliant Stain Buffer (BD Biosciences) and stained with Streptavidine^BV605 (BD Biosciences) for 10 min on ice. Cells were then washed and immediately analyzed on a BD FACSaria Fusion. The data collected was analyzed on FlowJo version 10. All antibodies used are listed in S1 Table.

Dolbec D., Lehoux M., de Beauville A.A., Zahn A., Di Noia J.M, & Segura M. (2024). Unmutated but T cell dependent IgM antibodies targeting Streptococcus suis play an essential role in bacterial clearance. PLOS Pathogens, 20(1), e1011957.

+ Open protocol

+ Expand

ROS and Cell Death Evaluation by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

CellROX Deep Red Flow Cytometry Assay Kit (Invitrogen Molecular Probes) was used to evaluate ROS production. Briefly, 5 × 10⁵ harvested cells were stained with CellROX Deep Red Reagent (1 µM) for 25 min at 37°C. For surface staining, cells were stained with anti-CD19-PE-Cy7 and anti-CD27-BV605 (all from BD Biosciences) for 15 min at 37°C. Finally, cells were stained with SYTOX Blue Dead Cell Stain (200 nM) for 15 min at 37°C to identify dead (SYTOX⁺) and viable (SYTOX⁻) cells. ROS production was evaluated as the percentage of viable naïve (CD19⁺CD27⁻) or memory (CD19⁺CD27⁺) B cells that stained positive for CellROX Deep Red probe (CellROX⁺ cells) (Figure 2A).
ROS fold increase induced by each single stimulus related to the unstimulated sample was expressed as a ratio: (single stimulus % CellROX⁺ cells)/(unstimulated % CellROX⁺ cells).
In vitro basal cell death was evaluated as the percentage of SYTOX⁺ naïve (CD19⁺CD27⁻) or memory (CD19⁺CD27⁺) B cells in unstimulated 24-h cultures (Supplementary Figure 1A).

Berman-Riu M., Cunill V., Clemente A., López-Gómez A., Pons J, & Ferrer J.M. (2024). Dysfunctional mitochondria, disrupted levels of reactive oxygen species, and autophagy in B cells from common variable immunodeficiency patients. Frontiers in Immunology, 15, 1362995.

+ Open protocol

+ Expand

Cytotoxicity Assays for Cell Lines and Primary Material

Check if the same lab product or an alternative is used in the 5 most similar protocols

Killing of cell lines was tested in standard ⁵¹chromium release assays as described previously [9 (link)]. Killing of primary material was analyzed in FACS-based cytotoxicity assays. 50,000 target cells were co-cultured with T cells in effector/target (E/T) ratio 3:1 overnight. After overnight culture cells were stained with specific antibody panels and SYTOX Blue Dead Cell Stain (Invitrogen by Thermo Fisher Scientific) was added prior to acquisition to identify living cells. Samples were acquired by FACS using fixed flow rates and stable acquisition was validated using Flow-Count Fluorospheres (Beckman Coulter). Co-cultures with activated B cells were stained using anti-CD3 (Alexa Fluor 700, BD Pharmingen), anti-CD19 (PE-Cy7, BD Pharmingen), anti-IgA (PE, Miltenyi), anti-IgG (FITC, DAKO) and anti-IgM (APC, BD). For primary MM, 50,000 patient-derived BM mononuclear cells were used as target cells. Co-cultures were stained with anti-CD3, anti-CD19 (APC, BD Pharmingen), anti-CD45 (FITC, BD), anti-CD38 (PE-Texas Red, Invitrogen), and anti-CD56 (PE-Cy7, BD Pharmingen). MM cells were defined as CD3^neg, CD19^neg, CD45^neg−int, CD38^pos, CD56^pos.

Meeuwsen M.H., Wouters A.K., Wachsmann T.L., Hagedoorn R.S., Kester M.G., Remst D.F., van der Steen D.M., de Ru A.H., van Hees E.P., Kremer M., Griffioen M., van Veelen P.A., Falkenburg J.H, & Heemskerk M.H. (2023). Broadly applicable TCR-based therapy for multiple myeloma targeting the immunoglobulin J chain. Journal of Hematology & Oncology, 16, 16.

+ Open protocol

+ Expand

Comprehensive Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mononuclear cells (5 × 10⁵) from peripheral blood or bone marrow aspirates were plated in 96-well convex bottom plates and treated with blocking solution (0.1% NaN₃, 2.0% FBS, 2% rabbit serum, and 5 mM EDTA) in the cold for 1 h. Cells were subsequently stained with monoclonal antibodies (mAb) at 4°C for 30 min; anti-CD56-PE (BD Biosciences) and anti-CD16-PerCP Cy5.5 (BioLegend, Inc.) were used to identify the different subpopulations of NK cells. To determine other populations of cells, anti-CD14-FITC (Santa Cruz Biotechnology, Inc.) was used to detect monocytes, anti-CD3-Pacific Blue for T-lymphocytes, and anti-CD19-PECy7 for B-lymphocytes (BD Biosciences). Cells were washed with staining buffer (0.1% NaN₃, 2.0% FBS, and 5 mM EDTA) and fixed with 1% formalin. In total, 20,000 events from the lymphocyte regions were acquired using a FACS Aria II cytometer (BD Biosciences) after analysis with their respective autofluorescence and isotype controls and analyzed using FlowJo version XV (Tree Star, Inc.).

Sánchez-Herrera D., Contreras-Ramos A., Jiménez-Hernández E., Medina-Sansón A., Giono-Cerezo S, & Maldonado-Bernal C. (2021). NK Cell Subpopulation Is Altered and the Expression of TLR1 and TLR9 Is Decreased in Patients with Acute Lymphoblastic Leukemia. Journal of Oncology, 2021, 5528378.

+ Open protocol

+ Expand

Quantifying HVEM Expression on B Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated whole CD4⁺ populations or isolated Tfh cells were stained with with AlexaFluor 647-conjugated anti-BTLA (BioLegend, 344520) and compared to Quantum AlexaFluor 647 MESF calibration beads (Bangs Laboratories) by flow cytometry. HVEM levels on B cells were determined by staining PBMCs from whole blood with anti-CD19-PE-Cy7 (BD Biosciences; 560911), anti-CD38-AlexaFluor 488 (BioLegend; 303511), anti-CD27-BrilliantViolet421 (BioLegend; 356417), anti-CD20-PE (BioLegend; 302305), and anti-HVEM-AlexaFluor 647 (BD Biosciences; 564411). B cell subsets were gated as follows: Activated (CD19+ CD20+ CD27+ CD38+), Memory (CD19+ CD20+ CD27+ CD38-), immature/transitional (CD19+ CD20+ CD27- CD38+), and plasmablasts (CD19+ CD20- CD27+ CD38+). HVEM intensity on each cell subset was converted to absolute protein numbers by reference to Quantum AlexaFluor 647 MESF calibration beads (Bangs Laboratories). Based on the mean HVEM expression on activated B cells of ~35,000/cell, minimum surface density was estimated at ~35 molecules/µm² assuming an upper limit of ~1000 µm² total area.

+ Open protocol

+ Expand

Splenic Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Spleens were harvested from untreated wild-type, TNFR1 KO and SPRET mice, and single cells were obtained by teasing the spleen apart by pressing it through a 100-μm cell strainer, eliminating clumps and debris. Cells were collected in 10 ml DPBS and were spin down (4°C, 7 min, 400× g). Cells were lysed with 1 ml of lysis buffer (8.3 g NH₄Cl, 1 g KHCO₃ and 200 μl 0.5 mol/l EDTA), washed and counted. A total of 1 × 10⁶ splenocytes were stained for 30 min at 4°C in the dark with the following anti-mouse antibodies: anti-TCRb-FITC, anti-CD19-PECy7, anti-CD11b-Pacific Blue, anti-Ly6C-APC, anti-Ly6G-AF700 (all from BD Biosciences) and anti-TNFR1-PE (BioLegend). Cells were washed and dissolved in FACS buffer. Fluorescent events were acquired using an LSR2 and analysed using FACSDiva software (BD Biosciences). Neutrophils were defined as SSC^highCD11b⁺Ly6G^highLy6C^high. After gating neutrophils (see Supplementary Fig S7), TNFR1⁺ cells were plotted in a histogram. The correct neutrophil population and TNFR1⁺ populations were gated using an FMO conditions without anti-Ly6G-AF700 and anti-TNFR1-PE antibodies, respectively. Analysis was performed using FlowJo software (Tree Star, Inc.)

Puimège L., Van Hauwermeiren F., Steeland S., Van Ryckeghem S., Vandewalle J., Lodens S., Dejager L., Vandevyver S., Staelens J., Timmermans S., Vandenbroucke R.E, & Libert C. (2015). Glucocorticoid-induced microRNA-511 protects against TNF by down-regulating TNFR1. EMBO Molecular Medicine, 7(8), 1004-1017.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!