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Eclipse 80i

Manufactured by National Instruments
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The Eclipse 80i is a lab equipment product by National Instruments. It is a precision instrument designed for laboratory applications, but its core function is not explicitly described.

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Lab products found in correlation

5 bromodeoxyuridine brdu, by Merck Group (2 mentions) Nis elements br software, by National Instruments (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Colcemid, by Thermo Fisher Scientific (1 mentions) Nis elements d4, by National Instruments (1 mentions) 3 3 dab hcl, by Merck Group (1 mentions) α sma, by Merck Group (1 mentions) E cadherin, by Santa Cruz Biotechnology (1 mentions) Pkh26 red fluorescence cell linker kit, by Merck Group (1 mentions) β tubulin antibody, by Merck Group (1 mentions) Permount sp15 500, by Thermo Fisher Scientific (1 mentions) Smz1500 microscope, by Nikon (1 mentions) Ez c1 viewer, by Nikon (1 mentions) Eclipse e600fn, by Nikon (1 mentions) Metamorph, by Molecular Devices (1 mentions) Microplate reader, by Bio-Rad (1 mentions) Smz1000, by Nikon (1 mentions) Powershot sx170 is, by Canon (1 mentions) Senescence cells histochemical staining kit, by Merck Group (1 mentions)

Spelling variants of this product

Eclipse 80i microscope (23 citations) Eclipse 80i fluorescence microscope (3 citations)

22 protocols using eclipse 80i

1

Histological Evaluation of Skin Tissue

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Skin was fixed in 4% paraformaldehyde and processed for paraffin embedment. Tissue sections (5 μm) were stained with haematoxylin and eosin (H&E) for histology evaluation. The stained sections were photographed using a Nikon Eclipse 80i microscope, and analysed with NIS‐Elements AR 2.10 imaging software.
Zhang Y., de Graaf N.P., Veldhuizen R., Roffel S., Spiekstra S.W., Rustemeyer T., Kleverlaan C.J., Feilzer A.J., Bontkes H., Deng D, & Gibbs S. (2021). Patch test–relevant concentrations of metal salts cause localized cytotoxicity, including apoptosis, in skin ex vivo. Contact Dermatitis, 85(5), 531-542.
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2

Metaphase Chromosome Analysis via BrdU

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106 cells were incubated with 9 μg/mL 5-bromodeoxyuridine (BrdU) (Sigma) for 48 hours, followed by 0.1 μg/ml colcemid (Thermo Fischer Scientific) treatment for 40 min before standard metaphase chromosome harvest69 . Images were acquired by Nikon eclipse 80i / NIS Elements D4.20.00. For each cell line, 50 metaphases were analyzed to determine the SCE frequency.
Su W.P., Ho Y.C., Wu C.K., Hsu S.H., Shiu J.L., Huang J.C., Chang S.B., Chiu W.T., Hung J.J., Liu T.L., Wu W.S., Wu P.Y., Su W.C., Chang J.Y, & Liaw H. (2017). Chronic treatment with cisplatin induces chemoresistance through the TIP60-mediated Fanconi anemia and homologous recombination repair pathways. Scientific Reports, 7, 3879.
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3

Quantifying Neuronal Markers in Brain

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Tissues stained for thionin, SMI-32, DCX, Calb, Calr, Parv, APP/Aβ (6E10), Aβ1–42, CP13, and PHF-1 were imaged using a Nikon Eclipse 80i and analyzed using NIS-Elements BR software. All cell density counts were performed in 10 different areas on each slide at 400 × magnification within SG layers II-III and IG layers V-VI, except for Calb which was counted at 200 × magnification (due to the low density of positive cells) and an average cell count was calculated for each layer per case. Additionally, all counts were normalized against thionin-stained cell numbers. APP/Aβ plaque number and load were examined at 100 × magnification and plaque load was calculated as a percentage of the area immunostained versus the area examined of 1.02 mm2 using NIS-Elements BR software. Counts were performed by an investigator blind to case demographics.
Utagawa E.C., Moreno D.G., Schafernak K.T., Arva N.C., Malek-Ahmadi M.H., Mufson E.J, & Perez S.E. (2022). Neurogenesis and neuronal differentiation in the postnatal frontal cortex in Down syndrome. Acta Neuropathologica Communications, 10, 86.
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4

Microtubule Dynamics in HeLa Cells

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HeLa cells were transfected with NuMA or GADPH siRNA (Life Technologies, Grand Island, NY) in oligofectamine for 72 h. Taccalonolide A was added to for 4 h after which cells were immediately fixed and microtubules visualized by indirect immunofluorescence for β-tubulin with a Nikon Eclipse 80i fluorescence microscope and NIS Elements software as previously described [29 (link)]. The distribution of cells in interphase, in mitosis with cortically localized tubulin, and in mitosis with asters were determined. A minimum of 800 cells were evaluated for each treatment condition.
Risinger A.L., Riffle S.M., Lopus M., Jordan M.A., Wilson L, & Mooberry S.L. (2014). The taccalonolides and paclitaxel cause distinct effects on microtubule dynamics and aster formation. Molecular Cancer, 13, 41.
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5

Measuring Cellular DNA Damage

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106 cells were incubated with 9μg/mL 5-bromodeoxyuridine (BrdU) (Sigma) for 48 hours, and SCE analysis was performed as described previously. Images were acquired by Nikon eclipse 80i / NIS Elements D4.20.00. For each cell line, 50 metaphases were analyzed to determine the SCE frequency.
Su W.P., Hsu S.H., Wu C.K., Chang S.B., Lin Y.J., Yang W.B., Hung J.J., Chiu W.T., Tzeng S.F., Tseng Y.L., Chang J.Y., Su W.C, & Liaw H. (2014). Chronic treatment with cisplatin induces replication-dependent sister chromatid recombination to confer cisplatin-resistant phenotype in nasopharyngeal carcinoma. Oncotarget, 5(15), 6323-6337.
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6

Fluorescence Microscopy of Bacteroid Samples

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One μL of each freshly prepared bacteroid sample was mixed with 1 μL of 50 μg mL−1 DAPI or with both 1 μL of 50 μg mL−1 DAPI and 1 μL of 100 μg mL−1 propidium iodide (PI), which are both DNA binding dyes. Samples were visualized at ×100 magnification under oil immersion using a Nikon Eclipse 80i fluorescence microscope with the NIS-Elements BR 4.00.01 software and a Digital Sight DS-U3 camera.
diCenzo G.C., Cangioli L., Nicoud Q., Cheng J.H., Blow M.J., Shapiro N., Woyke T., Biondi E.G., Alunni B., Mengoni A, & Mergaert P. (2022). DNA Methylation in Ensifer Species during Free-Living Growth and during Nitrogen-Fixing Symbiosis with Medicago spp.. mSystems, 7(1), e01092-21.
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7

Quantification of Cardiomyocyte Apoptosis

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TdT assay was performed using the kit (Promega, Madison, WI) according to the manufacturer’s instructions. Five sections from each left ventricle (LV) cut perpendicularly to the major axis of the heart were sampled. TUNEL positive cells were counted throughout the LV and were expressed as percentage of the total number of nuclei as determined by DAPI (Molecular probes, Pittsburgh, PA). TUNEL costaining with tropomyosin was used to assess the percentage of apoptotic cardiomyocytes. All images were captured in 600X magnification using bright field microscope (Nikon Eclipse 80i, NIS Elements version 4.3). Quantification was done by ImageJ software (bundled with 64-bit Java 1.8.0_112).
Kolpakov M.A., Sikder K., Sarkar A., Chaki S., Shukla S.K., Guo X., Qi Z., Barbery C., Sabri A, & Rafiq K. (2019). Inflammatory Serine Proteases Play a Critical Role in the Early Pathogenesis of Diabetic Cardiomyopathy. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 53(6), 982-998.
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8

Histological Analysis of C5 Samples

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Samples of C5 stage from each treatment were fixed in Davidson solution for 24–48 h, stored in a 70% ethanol solution until further normal histological processing. Parafin wax sections (5μm thick) were stained with H&E and were observed using Nikon® microscope type Eclipse 80i, and image analysis (NIS-Elements D 2.30 227) software.
Syafaat M.N., Azra M.N., Mohamad F., Che-Ismail C.Z., Amin-Safwan A., Asmat-Ullah M., Syahnon M., Ghazali A., Abol-Munafi A.B., Ma H, & Ikhwanuddin M. (2021). Thermal Tolerance and Physiological Changes in Mud Crab, Scylla paramamosain Crablet at Different Water Temperatures. Animals : an Open Access Journal from MDPI, 11(4), 1146.
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9

Visualizing Hydrogen Peroxide in Pea Roots

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We analyzed the primary and lateral roots of pea harvested after seven days of Cd treatment. Localization of H2O2 was performed by modifying the method of Thordal-Christensen et al. [57 (link)]. The fresh, hand-cut samples were placed in 1 mg/mL solution of 3,3′-DAB-HCl, pH 3.8 (Sigma), for 30 min at room temperature. Production of H2O2 was visualized as a reddish-brown coloration. We conducted tissue fixation of DAB stained tissues in 2.5% glutaraldehyde after rinsing slides for 10 min. Results were analyzed with Nikon microscopy (Eclipse 80i) and a NIS-Elements BR 3.1 program.
Głowacka K., Źróbek-Sokolnik A., Okorski A, & Najdzion J. (2019). The Effect of Cadmium on the Activity of Stress-Related Enzymes and the Ultrastructure of Pea Roots. Plants, 8(10), 413.
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10

Immunohistochemistry Staining Protocol

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Samples were processed and stained according to the manufacturer's instructions (Vectastain ABC Kit). Antibodies include: PTEN (Cell Signaling Technology (CST #9559 - 1:100)), pAKT(S473) #4060 (CST 1:200), Ki67 (1:500, Novus Biochemicals #NB110-89719), α-SMA (Sigma-Aldrich #A7607, 1:1000), E-cadherin (CST #3195 1:200), AR (Santa Cruz Biotechnology (SCBT) #N-20, 1:400), p65 (CST #6956 1:200). TUNEL assay performed using DeadEnd Fluorimetric TUNEL Assay (Promega, #G3250). Photomicrographs were taken using a Nikon Eclipse 80i microscope and acquired using NIS-Elements BR 3.2. Investigators were not blinded during immunohistochemical analysis.
Rodriguez M., Siwko S., Zeng L., Li J., Yi Z, & Liu M. (2015). Prostate Specific G-protein coupled Receptor collaborates with loss of PTEN to promote prostate cancer progression. Oncogene, 35(9), 1153-1162.
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