The largest database of trusted experimental protocols

Isl 1 antibody

Manufactured by R&D Systems

The Isl-1 antibody is a research-use antibody that recognizes the Isl-1 (Insulin Gene Enhancer Protein ISL-1) protein. Isl-1 is a transcription factor involved in the development and differentiation of various cell types, including pancreatic beta cells, motor neurons, and cardiac cells. The Isl-1 antibody can be used to detect and analyze the expression of the Isl-1 protein in biological samples.

Automatically generated - may contain errors

2 protocols using isl 1 antibody

1

Immunohistochemical Analysis of Cardiac Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissues were processed as described previously (12 (link)). Briefly, rat hearts were excised after hemodynamic data collection under anesthesia and perfused with 4% paraformaldehyde. Human tissue was fixed with 4% paraformaldehyde. Tissues were cryo-preserved using 30% sucrose and embedded in OCT (TissueTek). Sections were cut to 7µm, using a commercial cryostat, and stained for isolectin B4 (Invitrogen; Carlsbad, CA), α-SMA (Sigma), sarcomeric-α-actin (Santa Cruz Biotechnology; Santa Cruz, CA), anti-human nuclei (Millipore; Billerica, MA), or c-kit+ (BD Biosciences) primary antibodies. For Isl-1 staining, the cells were fixed with 4% PFA, permeabilized, overnight incubated with Isl-1 antibody (R & D Systems), incubated with FITC labeled secondary antibody. Cells and tissue sections were counterstained with DAPI (4’,6-diamidino-2-phenylindole) nuclear stain (Sigma; St Louis, MO).
+ Open protocol
+ Expand
2

Immunohistochemical Analysis of Heart Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Heart biopsy specimens were fixed in 4% para-formaldehyde and then cryopreserved using 30% sucrose and embedded in OCT (Sakura Finetek, Zoeterwoude, The Netherlands). Sections were cut to 7 μm, using a commercial cryostat, and stained for sarcomeric-α-actin (Santa Cruz Biotechnology, Santa Cruz, CA), transcription factor GATA-4 (Santa Cruz Biotechnology), or c-kit+ (BD Biosciences, Franklin Lakes, NJ) primary antibodies. For islet-1 (ISL-1) staining, tissue sections were permeabilized, incubated overnight with ISL-1 antibody (R&D Systems, Minneapolis, MN), and incubated with fluorescein isothiocyanate–labeled secondary antibody. Tissue sections were counterstained with nuclear 4,6-diamidino-2-phenylindole stain (Sigma).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!