Bovine anti mouse igg tr

Recombinant human TGF-β1 (240-B) and monoclonal anti-TGF-β1 antibody were purchased from R&D Systems (Minneapolis, MN, USA). Mouse anti-human FHL2, used for western blotting, was purchased from MBL International Incorporation (Woburn, Japan). Rabbit anti-human KLF8 antibody, used for western blotting and IHC, was purchased from Aviva Systems Biology (San Diego, CA, USA). Prediluted rabbit anti-human vimentin, N-cadherin and E-cadherin, KLF8 and mouse anti-human glyceraldehyde-3- phosphate dehydrogenase (GAPDH) antibodies, used for western blotting and/or immunohistochemistry, were purchased from Abcam (Cambridge, UK). Bovine anti-mouse IgG-TR and goat anti-rabbit IgG-FITC antibodies purchased from Santa Cruz (Santa Cruz, CA, USA). Goat anti-rabbit immunoglobulins/HRP, rabbit anti-mouse immunoglobulins/HRP, and normal mouse IgG antibodies were purchased from Dako (Carpinteria, CA, USA).
The human colon cancer cell lines HT29, SW480, SW1116, Caco2, DLD1, SW620 and LoVo were obtained from American Type Culture Collection (ATCC, Rockville, MD, USA) and cultured as previously described [16 (link)].

Rhodamine-phallotoxin was purchased from Molecular Probes (Eugene, OR, USA). Mouse anti-human FHL2 used for western blot were a product of the MBL international incorporation (11–134, MBL International Incorporation, Woburn, Japan). Rabbit anti-human FHL2 antibody was used for IHC and was purchased from Abcam (Cambridge, UK). Mouse anti-FOXK1 (G-4), Vimentin (E-5), E-Cadherin (H-108), GAPDH (G-9) and bovine anti-mouse IgG-TR and goat anti-rabbit IgG-FITC were purchased from Santa Cruz (Santa Cruz, CA, USA). Mouse anti-human Flag were a product of Sigma (St Louis, MO, USA). Goat anti-rabbit immunoglobulins/HRP, rabbit anti-mouse immunoglobulins/HRP, normal mouse and rabbit IgG were all products of Dako (Carpinteria, CA, USA).
Human colon cancer cell lines SW480, Caco2 and SW620 were all maintained in our laboratory as previously described.^{26 (link), 27 (link)} The cells were cultured in RPMI 1640 (Life Technologies, Inc., Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum, 100 μg/ml streptomycin and 100 units/ml penicillin in a humidified incubator at 37 °C with an atmosphere of 5% CO₂.