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Human umbilical vein endothelial cells huvecs

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Human umbilical vein endothelial cells (HUVECs) are a primary cell line derived from the endothelium of human umbilical veins. They are widely used in research to study vascular biology, angiogenesis, and related processes.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) Egm 2, by Lonza (6 mentions) Huvecs, by Lonza (4 mentions) Mda mb 231, by American Type Culture Collection (4 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Mcf 7, by American Type Culture Collection (3 mentions) Endothelial cell basal medium 2, by Lonza (3 mentions) Penicillin streptomycin, by Lonza (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions) Sodium pyruvate, by Lonza (2 mentions) R3 igf 1, by Lonza (2 mentions) Gentamicin amphotericin b, by Lonza (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Ebm 2, by Lonza (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Egm 2 medium, by Lonza (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Egm bulletkit, by Lonza (2 mentions)

Close spelling variants of this product

HUVECs (1045 citations) HUVEC (396 citations) HUVEC cells (37 citations) HUVEC human umbilical vein endothelial cells (2 citations)

70 protocols using human umbilical vein endothelial cells huvecs

1

Culturing Human Endothelial and Pericyte Cells

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Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza (Basel, Switzerland), and human brain pericytes were purchased from ScienCell (Carlsbad, CA). Cultures were grown to 70% confluence under standard conditions (37°C, 5% CO2).
Tabit C.E., Coplan M.J., Chen P., Jeevanandam V., Uriel N, & Liao J.K. (2017). Tumor necrosis factor-α levels and non-surgical bleeding in continuous-flow left ventricular assist devices. The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation, 37(1), 107-115.
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2

Inhibition of Autotaxin and LPA Receptor

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1-oleoyl-LPC (18:1) was purchased from Avanti Polar Lipids (Alabaster, AL, USA) and diluted in Dulbecco’s Modified Eagle’s Medium (Lonza, Walkersville, MD, USA) including 0.1% bovine serum albumin (Sigma Aldrich, St. Louis, MO, USA). The selective ATX inhibitor PAT-04821 (link),22 (link),24 (link) and the LPA1-selective small molecule antagonist AM152 (Example 1 in patent application US/20120015991) were synthesized by PharmAkea Inc (San Diego, CA, USA). Sodium pyruvate, NEAA mixture and Penicillin/Streptomycin were purchased from Lonza. L-glutamine was from CellGenix (Portsmouth, NH, USA). Dulbecco’s Modified Eagle’s Medium and fetal bovine serum (FBS) were from Thermo Fisher Scientific (Waltham, MA, USA). Charcoal-stripped FBS was prepared as previously described. In brief, 10 mL of FBS was incubated with 1 g of activated charcoal (Sigma Aldrich) overnight at 4 °C42 (link). After centrifugation at 2000 × g for ten minutes, the supernatant was filtered for the experiments. DMSO was from Sigma Aldrich. Mouse primary renal fibroblasts were obtained from Cell Biologics (Chicago, IL, USA). Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza. Recombinant ATX was purchased from R&D systems (Minneapolis, MN, USA).
Sakai N., Bain G., Furuichi K., Iwata Y., Nakamura M., Hara A., Kitajima S., Sagara A., Miyake T., Toyama T., Sato K., Nakagawa S., Shimizu M., Kaneko S, & Wada T. (2019). The involvement of autotaxin in renal interstitial fibrosis through regulation of fibroblast functions and induction of vascular leakage. Scientific Reports, 9, 7414.
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3

Glioma Cells and Glioblastoma Stem Cell Culture

Cited 2 times
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Human U87 glioma cells were obtained from American Type Culture Collection (ATCC, Manassas, VA) and grown in complete Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10 % fetal calf serum at 37 °C and 5 % CO2. Human GSCs MGG4, MGG8, MGG18, BT74 were isolated as previously described [28 (link),30 (link)], and maintained as spheres in serum-free medium containing 20 ng/mL recombinant human EGF (R&D systems) and 20 ng/mL recombinant human FGF2 (Peprotech). GSCs were passaged by dissociating neurospheres using the Neuro-Cult Chemical Dissociation Kit (StemCell Technologies). Mouse 005 GSCs were provided by Dr. I. Verma (Salk Institute for Biological Studies, La Jolla, CA) [33 (link), 34 (link)]. Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza. Human brain microvascular endothelial cells (HBMECs) were obtained from Dr. Ken Arai (MGH). Axitinib (Pfizer Inc) was provided by Pfizer and dissolved in DMSO as a 25 mM stock solution for in vitro studies. The final concentrations added to cells had less than 0.5 % DMSO, which was nontoxic to cells.
Lu L., Saha D., Martuza R.L., Rabkin S.D, & Wakimoto H. (2014). Single agent efficacy of the VEGFR kinase inhibitor axitinib in preclinical models of glioblastoma. Journal of neuro-oncology, 121(1), 91-100.
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4

Murine and Human Cell Culture Protocols

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The murine dendritic cell line, JAWS II, was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). JAWS II was cultured in the presence of alpha-minimum essential media (Lonza, Basel, Switzerland) complemented with 20% fetal bovine serum (FBS, Life Technologies; Grand Island, NY, USA), 1% penicillin-streptomycin (Sigma-Aldrich, St. Quentin Fallavier, France), 4 mM L-glutamine (Lonza), 1 mM sodium pyruvate (Lonza), and 5 ng/mL of Granulocyte-Macrophage Colony Stimulating Factor (Miltenyi Biotec; San Diego, CA, USA). The cells were maintained in culture in a humified incubator at 37 °C and 5% CO2.
The MDA-MB-231 human breast cancer cells were purchased from ATCC and cultured at 5% CO2 at 37 °C in Dulbecco’s modified Eagle media (Lonza), supplemented with 10% FBS, 2 mM glutamine, 10% penicillin-streptomycin.
Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza and grown at 37 °C and 5% CO2 in endothelial basal cell growth medium, supplemented with 2% FBS, 1% streptomycin-penicillin, 0.1% ascorbic acid, 0.1% human epidermal growth factor, 0.1% heparin, 0.1% VEGF, 0.1% gentamicin-amphotericin B, 0.04% hydrocortisone, 0.4% human bFGF, and 0.1% R3-IGF-1 (all from Lonza).
Salek A., Selmi M., Barboura M., Martinez M.C., Chekir-Ghedira L, & Andriantsitohaina R. (2022). Enhancement of the In Vitro Antitumor Effects of Berberine Chloride When Encapsulated within Small Extracellular Vesicles. Pharmaceutics, 14(9), 1913.
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5

Culturing Human Umbilical Vein Endothelial Cells

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Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza (Walkersville, MD, USA) and grown for 2–6 passages in EGM2 culture media (Lonza, Basel, Switzerland) supplemented with 2% fetal bovine serum (FBS). The cultures were maintained in a humidified atmosphere with 5% CO2 at 37 °C. HEK-293 T cells were cultured in DMEM with 10% FBS.
Lin H.H., Kuo M.W., Fan T.C., Yu A.L, & Yu J. (2023). YULINK regulates vascular formation in zebrafish and HUVECs. Biological Research, 56, 7.
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6

Culturing Human Umbilical Vein Endothelial Cells

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Human umbilical vein endothelial cells (HUVECs) derived from the umbilical cords of female donors were purchased from Lonza. The HUVECs were expanded in Endothelial Growth Medium (EGM-2; Lonza) on gelatin-treated culture dishes at 37°C and were passaged upon reaching 80% confluency by 0.05% trypsin-EDTA treatment.
Luo J., Qin L., Zhao L., Gui L., Ellis M.W., Huang Y., Kural M.H., Clark J.A., Ono S., Wang J., Yuan Y., Zhang S.M., Cong X., Li G., Riaz M., Lopez C., Hotta A., Campbell S., Tellides G., Dardik A., Niklason L.E, & Qyang Y. (2020). Tissue Engineered Vascular Grafts with Advanced Mechanical Strength from Human iPSCs. Cell stem cell, 26(2), 251-261.e8.
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7

Culturing Human Umbilical Vein Endothelial Cells

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Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza (Verviers, Belgium). Cells were cultured in endothelial cell growth basal medium (Lonza, CC-3121, Verviers, Belgium) supplemented with SingleQuots™ (Lonza, CC-4133, Verviers, Belgium) and maintained at 37°C in a humidified atmosphere containing 5% CO2. HUVECs were used at passages 2–5.
Haguet H., Bouvy C., Delvigne A.S., Modaffari E., Wannez A., Sonveaux P., Dogné J.M, & Douxfils J. (2020). The Risk of Arterial Thrombosis in Patients With Chronic Myeloid Leukemia Treated With Second and Third Generation BCR-ABL Tyrosine Kinase Inhibitors May Be Explained by Their Impact on Endothelial Cells: An In-Vitro Study. Frontiers in Pharmacology, 11, 1007.
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8

Culturing Primary Endothelial Cells

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Human umbilical vein endothelial cells (HUVECs) and human retinal microvascular endothelial cells (HRMECs) were purchased from Lonza (Walkersville, MD, USA) and cultured in EGM-2 MV medium (Lonza) containing 5% FBS at 37 °C in 5% CO2.
Shiono A., Sasaki H., Sekine R., Abe Y., Matsumura Y., Inagaki T., Tanaka T., Kodama T., Aburatani H., Sakai J, & Takagi H. (2020). PPARα activation directly upregulates thrombomodulin in the diabetic retina. Scientific Reports, 10, 10837.
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9

Comparative Evaluation of Pancreatic Cancer Cells

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Human pancreatic adenocarcinoma cells PANC-1 were purchased from ATCC (Manassas, VA, USA). PANC-1 cells were grown under standard culture conditions (37°C, 95% humidified air, and 5% CO2) in RPMI-1640 medium and supplemented with 10% FBS and 1% Penicillin-Streptomycin from Thermo Fisher Scientific (Waltham, MA, USA). Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza (Walkersville, MD, USA) and cultured in EBM-2 (endothelial cell growth basal medium 2) supplemented with endothelial cell growth medium 2 (EGM-2) Bullet kit under standard culture conditions. Antibody for Ki-67 (#ab16667) was from Abcam (Cambridge, MA, USA). The cleaved caspase 3 (Asp175) (#9661) antibody was from Cell Signaling Technology (Beverly, MA, USA), and the antibody for cluster of differentiation 31 (CD-31) (#sc-376764) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Phenol red and LDEV-free Matrigel (#356237) were obtained from Corning Life Sciences (Corning, NY, USA), and 0.4% trypan blue dye (#15250061) was from Thermo Fisher Scientific.
Kandhari K., Paudel S., Raina K., Agarwal C., Kant R., Wempe M.F., O’Bryant C, & Agarwal R. (2021). Comparative Pre-clinical Efficacy of Chinese and Indian Cultivars of Bitter Melon (Momordica charantia) against Pancreatic Cancer. Journal of Cancer Prevention, 26(4), 266-276.
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10

Breast Cancer Cell Line Protocols

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GCS, suramin sodium salt (SM), doxorubicin (DOX), nuclei isolation kit (nuclei EZ prep), eosin Y, and BCA kit were purchased from Sigma-Aldrich (St. Louis, MO). Acidic fibroblast growth factor (aFGF) and bFGF were purchased from PeproTech (NJ, USA). Luciferin was obtained from Merck Millipore. Gill’s Hematoxylin No. 2 was purchased from VWR. AntiCD31 antibody (ab28364) was purchased from Abcam. Dako envision kit was purchased from Dako (CA, USA).
The human breast cancer cell line MDA-MB-231 was obtained from the American Type Culture Collection (ATCC, Manassas, VA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/mL of penicillin G sodium, and 100 μg/mL of streptomycin, and 10 ng/mL of a/b FGF. Human umbilical vein endothelial cells (HUVECs) were obtained from Lonza (MD, USA) and incubated with complete EGM. Murine breast cancer cell line (E0771) was cultured in DMEM supplemented with 10% heat-inactivated FBS, 100 U/mL of penicillin G sodium, and 100 μg/mL of streptomycin. All these cells were maintained at 37°C in an atmosphere of 5% CO2 and 95% air. MDA-MB-231/Luc-GFP cells, which stably express luciferase and green fluorescent protein, were a kind gift from Dr. Shirin Bonni (Faculty of Medicine, University of Calgary).
Cheng B., Gao F., Maissy E, & Xu P. (2018). Repurposing suramin for the treatment of breast cancer lung metastasis with glycol chitosan-based nanoparticles. Acta biomaterialia, 84, 378-390.
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