PerCP-Cy 5.5 Mouse lineage cocktail, APC c-Kit, APC Gr-1, PE B220, APC anti-BrdU and Fc block (CD16/CD32) were all from BD Pharmingen. Pacific blue anti-mouse Ly-6A/E (Sca-1) was purchased from Biolegend. PE F4/80, APC CD11b and APC Annexin V were from eBioscience.
Apc gr 1
Manufactured by BD
Sourced in United States
The APC Gr-1 is a laboratory equipment designed for cell analysis and sorting. It utilizes flow cytometry technology to provide accurate and efficient cell identification and separation. The core function of the APC Gr-1 is to facilitate the analysis and sorting of various cell types based on their physical and fluorescent properties.
Automatically generated - may contain errors
Lab products found in correlation
F4 80 pe, by Thermo Fisher Scientific (2 mentions)
Fc block cd16 cd32, by BD (1 mentions)
C kit apc, by BD (1 mentions)
B220 pe, by BD (1 mentions)
Anti brdu apc, by BD (1 mentions)
Cd11b apc, by Thermo Fisher Scientific (1 mentions)
Annexin 5 apc, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Cd3 fitc, by BD (1 mentions)
Cd11b fitc, by BD (1 mentions)
Cd45 bv421, by BD (1 mentions)
Cd8 pe, by BD (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Ly6g fitc, by BD (1 mentions)
Cd45 pe cy7, by BD (1 mentions)
Cd11b apc cy7, by BD (1 mentions)
Wright giemsa staining, by Merck Group (1 mentions)
Cd11b microbead, by Miltenyi Biotec (1 mentions)
3 protocols using apc gr 1
1
Multiparametric Analysis of Hematopoietic Cells
2
Mouse OSCC Xenograft Immune Analysis
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Mouse OSCC cell line SCC-7 was cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, USA) supplemented with 10% fetal bovine serum (Gibco); 0.25% trypsin was used for cell passage. Antibodies BV421-CD45, FITC-CD3 (BD Pharmingen, USA), PE-CD8 (BD Pharmingen), APC-PD-1 (BD Pharmingen), FITC-CD11b (BD Pharmingen) and APC-Gr-1 (BD Pharmingen) were used to detect the proportion of immune cells of tumor tissues in mouse OSCC xenografts. Antibody CD166 (CST, USA) was used to inject into peri-tumor tissues in mice.
3
Isolation and Characterization of Tumor-Infiltrating Myeloid Cells
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Tumors were harvested from MMTV-PyVT transgenic mice or MCaP0008 tumor-bearing mice. Tumor tissues were then minced and digested at 37°C for 45 min with DMEM containing collagenase type 1A (1.5 mg/ml), hyaluronidase (1.5 mg/ml), and DNase (20 U/ml). TiMDSCs and TAMs were enriched by CD11b-microbeads (Miltenyi, Germany). Enriched cells were then stained with PE-F4/80 (eBioscience), FITC-Ly6G, PE-Cy7-CD45, APC-Gr1, APC-Cy7-CD11b (BD Biosciences) and isolated by flow sorting. 50,000 tiMDSCs were subjected to cytospin preparation (Thermo Shandon), and were fixed and stained with a Wright Giemsa Staining according to the manufacturer's instructions (Sigma-Aldrich).
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