Taqman r fast universal pcr master mix

Manufactured by Thermo Fisher Scientific

Sourced in Italy

TaqMan (R) Fast Universal PCR Master Mix is a ready-to-use solution for real-time PCR amplification. It contains all the necessary components for efficient DNA/RNA detection and quantification, including a hot-start DNA polymerase, dNTPs, and optimized buffer system.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (2 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions) 7900ht fast real time pcr system, by Thermo Fisher Scientific (1 mentions) Nanodrop nd 100 spectrophotometer, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions)

Close spelling variants of this product

TaqMan Fast Universal PCR Mix TaqMan Universal PCR master Taqman universal mix Fast Taqman mix TaqMan Master Mix Universal Master Mix PCR Master Mix

2 protocols using taqman r fast universal pcr master mix

Prostate Transcriptome Analysis Protocol

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Prostates were lysed with TRIzol (Invitrogen, Life Technologies, Monza, Italy) and RNA was purified by phenol/chloroform extraction followed by loading onto RNeasy MINI kit (Qiagen, Milan, Italy). On-column DNAse treatment was performed. RNA purity and yield was assessed using NanoDrop ND-100 Spectrophotometer (NanoDrop Technologies, Wilmington, DE).
RNA was Reverse Transcribed using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). RT-qPCR reaction was prepared using TaqMan (R) Fast Universal PCR Master Mix and run on a 7900 HT Fast Real-time PCR System (Applied Biosystems, Life Technologies, Monza, Italy) as described previously [52 (link)]. The following TaqMan(R) probes were used: Gapdh (Mm99999915_g1), Ccne2 (Mm00438077_m1), Ccnd1 (Mm00432359_m1), E2f2 (Mm00624964_m1), Ddc (Mm00516688_m1), Syp (Mm00436850_m1). Expression values were normalized to internal control (Gapdh) and to control sample (TRAMP prostate) calculating the ΔΔCT.

Mauri G., Jachetti E., Comuzzi B., Dugo M., Arioli I., Miotti S., Sangaletti S., Di Carlo E., Tripodo C, & Colombo M.P. (2015). Genetic deletion of osteopontin in TRAMP mice skews prostate carcinogenesis from adenocarcinoma to aggressive human-like neuroendocrine cancers. Oncotarget, 7(4), 3905-3920.

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Quantification of Cytokine Gene Expression

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Total RNA was extracted from PBMC or from flow sorted PBMC subsets in Trizol according to the manufacturer's instructions (Invitrogen). Total RNA (1 μg) was reverse-transcribed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). qRT-PCR was carried out using Taqman^R Fast Universal PCR Master Mix using commercially available FAM-MGB primer probes (Taqman^R Gene Expression Assay, Applied Biosystems) for IL5 (Hs00174200_m1), IL13 (Hs00174379_m1), IL4 (Hs00174122_m1) and IFN-γ (Hs00174143_m1). All reactions were performed in triplicate, and the relative expression was normalized to the corresponding 18S rRNA cycle threshold (C_t). Lack of expression was defined as a C_t of more than 35 cycles (for IL5) despite the presence of sufficient cDNA (18S C_t <12). Data are expressed as 1/ΔC_t.

Babu S.P., Chen Y.Y., Bonne-Annee S., Yang J., Maric I., Myers T.G., Nutman T.B, & Klion A.D. (2017). Dysregulation of interleukin 5 expression in familial eosinophilia. Allergy, 72(9), 1338-1345.

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